摘要/Abstract
![](http://sioc-journal.cn/Jwk_yjhx/fileup/0253-2786/PIC/cjoc201904066_pic.jpg)
采用溶剂提取法和正、反相硅胶柱色谱及高效液相等多种色谱技术对小连翘化学成分进行分离纯化,并根据理化性质和现代波谱技术对其进行结构鉴定.从其氯仿提取物分离3个结构类型分别属于吡喃酮、黄酮和脂肪醇的新化合物以及15个已知化合物.以脂多糖(LPS)诱导RAW264.7细胞建立炎症模型,采用Griess法测定NO的分泌量,其中hyperiflavone(2)、isocudraniaxan-thone B(5)和2-deprenylrheediaxanthone B(6)对NO分泌表现出一定的抑制活性,其IC50分别为12.90,16.24和9.86 μmol·L-1.
关键词: 金丝桃属, 小连翘, 呫吨酮, 抗炎活性
The chemical constituents of Hypericum erectum Thunb were isolated and purified by solvent extraction method, the normal and reverse phase silica gel column chromatography and semi-preparative high performance liquid chromatography. Their structures were elucidated by their physicochemical property and extensive analysis of spectroscopic data. Three new compounds which belong to α-pyranone, flavonoid and aliphatic alcohol respectively, together with 15 known compounds, were isolated from the chloroform extract of H. erectum. Inflammatory cell model was constructed by lipopolysaccharide (LPS)-stimulated RAW264.7 cell line. NO production inhibitory effects of all tested compounds were measured by Griess method. Hyperiflavone (2), isocudraniaxan-thone B (5) and 2-deprenylrheediaxanthone B (6) exhibited moderate NO production inhibitory activities with the IC50 values of 16.24, 9.86 and 12.90 μmol·L-1, respectively.
Key words: Hypericum Linn, Hypericum erectum Thunb, xanthone, anti-inflammatory activity
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