摘要/Abstract

亚硝酰氢（HNO）是一氧化氮单电子还原并质子化的产物，具有重要的生物学意义.以4-（2-氨基乙基）-吗啉作为溶酶体靶向基团，1，8-萘二甲酰亚胺作为双光子荧光团，三苯基膦作为HNO识别基团，构建了一个能够特异性定位于溶酶体的打开型双光子荧光探针Lyso-HNO.研究结果表明，该探针响应迅速，对HNO表现出良好的选择性，较高的灵敏性，检测极限可达202 nmol·L^-1.该探针可对HeLa细胞溶酶体外源HNO进行双光子荧光成像研究.
关键词: 溶酶体, 亚硝酰氢, 双光子, 细胞成像
Nitroxyl (HNO), which is the one-electron reduced and further protonated form of nitric oxide, plays important biological functions. A lysosome-targeted dual-photon HNO fluorescent probe (Lyso-HNO), which contains 4-(2-amino-ethyl)morpholine as lysosomal-targetable groups, 1,8-naphthalimide as two-photon fluorophore and triphenylphosphine as HNO reaction site, was synthesized and characterized. The recognition behaviors of Lyso-HNO to HNO were investigated. The results showed that Lyso-HNO exhibited good selectivity and sensitivity to HNO with fast response,. and the detection limit of Lyso-HNO to HNO was estimated to be 202 nmol·L^-1. The probe can be applied to bioimaging exogenous lysosomal HNO by two-photon fluorescence confocal microscopy.
Key words: lysosome, dual-photon, nitroxyl, bioimaging


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