槐定碱抑制人胃癌MKN45细胞的增殖并促进其凋亡
陈旭东, 华新宇^*, 孔秀敏, 王晓兰
漯河医学高等专科学校组织胚胎学教研室，漯河 462002
摘要
本研究旨在探讨槐定碱对人胃癌MKN45细胞增殖和凋亡的影响及其可能的作用机制。将人胃癌MKN45细胞分为对照组和槐定碱组(设立6个浓度亚组)。用MTT法检测MKN45细胞增殖情况，用免疫细胞化学染色法和Western blot观察细胞高迁移率组蛋白3 (high mobility group-box 3, HMGB3)的蛋白表达，用Hoechst 33342染色法观察细胞形态变化，用流式细胞术检测细胞凋亡。结果显示，槐定碱作用48 h能显著抑制MKN45细胞的增殖，作用呈剂量依赖性。与对照组相比，槐定碱作用48 h后MKN45细胞的HMGB3蛋白表达显著减少(P < 0.05)，细胞凋亡率显著升高(P < 0.05)。以上结果提示，槐定碱作用48 h可抑制人胃癌MKN45细胞的增殖，促进其凋亡，其机制可能与HMGB3表达下调有关。

关键词： 槐定碱; HMGB3; 胃癌; 增殖; 凋亡
分类号：R332;R338

Sophoridine inhibits the proliferation of human gastric cancer MKN45 cells and promotes apoptosis
CHEN Xu-Dong, HUA Xin-Yu^*, KONG Xiu-Min, WANG Xiao-Lan
Department of Histology and Embryology, Luohe Medical College, Luohe 462002, China
Abstract
The purpose of the study was to investigate the effect of sophoridine on the proliferation and apoptosis of human gastric cancer MKN45 cells and the possible mechanism. MKN45 cells were randomly divided into control and sophoridine (including 6 subgroups) groups. Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) colorimetric method. The protein expression of high mobility group-box 3 (HMGB3) was observed by immunocytochemical staining and Western blot. Hoechst 33342 staining method was used to observe the morphological changes of cells treated with sophoridine. Apoptosis was detected by flow cytometry. The results showed that the proliferation of cells was inhibited by 48-hour treatment of sophoridine in a dose-dependent manner. Compared with control group, sophoridine group showed decreased HMGB3 protein expression and increased apoptotic rate. These results suggest that sophoridine can inhibit the proliferation of MKN45 cells and promote their apoptosis, which may be related to down-regulation of HMGB3 protein expression.

Key words: sophoridine; high mobility group-box 3; gastric cancer; proliferation; apoptosis

收稿日期：2018-01-15　　录用日期：2018-07-11
通讯作者：华新宇　　E-mail: 83106761@qq.com
DOI: 10.13294/j.aps.2018.0052
引用本文：
陈旭东, 华新宇, 孔秀敏, 王晓兰. 槐定碱抑制人胃癌MKN45细胞的增殖并促进其凋亡[J]. 生理学报 2018; 70 (4): 391-396.
CHEN Xu-Dong, HUA Xin-Yu, KONG Xiu-Min, WANG Xiao-Lan. Sophoridine inhibits the proliferation of human gastric cancer MKN45 cells and promotes apoptosis. Acta Physiol Sin 2018; 70 (4): 391-396 (in Chinese with English abstract).



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