职务： 博士生导师
联系电话：
办公地址： A223
电子邮箱： dechenjiang@nju.edu.cn

课题组主页：





个人简介


南京大学化学化工学院及生命分析化学国家重点实验室教授，博士生导师，国家****基金获得者，教育部青年****。于2000、2003和2008年分别在南京大学、复旦大学和美国凯斯西储大学获学士、硕士和博士学位。

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工作经历


2008年10月至2011年5月在美国北卡莱罗拉州大学教堂山分校从事博士后研究。2011年5月加入南京大学化学化工学院，任单细胞分析课题组长。


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研究方向


课题组致力于发展新型电化学方法及装置，实现通用型的单细胞和亚细胞分析，为单细胞异质性研究提供研究工具和科学数据。主要研究方向包括：
(1) 单细胞试剂盒分析
细胞内生物分子的含量和活性共同决定着诸多细胞行为。目前的单细胞分析侧重于单个活细胞中生物分子含量的检测，而较少涉及分子活性（尤其是酶分子）的定量测定；对于胞内单个细胞器中生物分子活性的测量则更少见到。这一研究现状阻碍了人们对于生物分子活性与细胞行为之间关联度的认知。造成该研究不足的主要原因在于测定单细胞中生物分子活性的方法缺乏普适性，需个体化设计检测探针，增加了测量难度。近年来，课题组提出“单细胞试剂盒”的分析策略及装置，将测定生物分子活性的试剂盒组分输运进单个活细胞，完成“单细胞试剂盒”或“单细胞器试剂盒”的组装，应用于单细胞及单细胞器中多种酶分子活性的测定。























Recent work:
1. Pan R, Xu M, Jiang D*, Burgess JD, Chen HY. Nanokit for single-cell electrochemical analyses.Proc Natl Acad Sci USA. 2016, 113(41):11436-11440.
2. Pan R, Xu M, Burgess JD, Jiang D*, Chen HY. Direct electrochemical observation of glucosidase activity in isolated single lysosomes from a living cell.Proc Natl Acad Sci USA. 2018, 115(16):4087-4092.
3. Pan R, Hu K, Jiang D*, Samuni U, Mirkin MV*. Electrochemical Resistive-Pulse Sensing.J Am Chem Soc. 2019, 141(50):19555-19559.
4.Pan R, Hu K, Jia R, Rotenberg SA, Jiang D*,Mirkin MV*.Resistive-Pulse Sensing Inside Single Living Cells.J Am Chem Soc.2020, 142(12):5778-5784.


(2) 单细胞电化学发光成像分析

鉴于单细胞异质性的存在，高通量测量并统计分析单个细胞在生理/病理过程中生物分子的含量变化，对于精准理解生命过程的化学基础及疾病的早期诊断至关重要。目前，高通量单细胞分析主要通过设计并合成特异性的光学无机或有机探针，实现对单个细胞内生物分子的检测。而生物试剂盒利用一系列生化反应，转化目标物为光、电可以检测的分子，可对细胞群中各种生物分子进行高特异性的检测。课题组利用电化学发光技术高灵敏、高通量及装置简单（无需激发光源）的优点，提出并建立微尺度“单细胞试剂盒”电化学发光方法，实现高通量单细胞电化学检测。



















Recent work:

1.Zhang J, Jin R, Jiang D*, Chen HY. Electrochemiluminescence-Based Capacitance Microscopy for Label-Free Imaging of Antigens on the Cellular Plasma Membrane.J Am Chem Soc. 2019, 141(26):10294-10299.
2. Zhang J, Arbault S, Sojic N*, Jiang D*. Electrochemiluminescence Imaging for Bioanalysis.Annu Rev Anal Chem2019, 12;12(1):275-295.
3. Jin R, Huang YC, Chen L, Lu HY, Jiang D*, Chen HY.In situ observation of heterogeneous charge distribution at the electrode unraveling the mechanism of electric field-enhanced electrochemical activity. Chem. Sci. 2020, 11, 4158-4163.
4. Wang Y, Jin R, Sojic N*, Jiang D*, Chen HY. Intracellular Wireless Analysis of Single Cells by Bipolar Electrochemiluminescence Confined in a Nanopipette.Angew Chem Int Ed Engl.2020 doi: 10.1002/anie..





学术成果


69.Liu G, Kolodziej C, Jin R, Qi S, Lou Y*, Chen J, Jiang D*, Zhao Y*, Burda C*.MoS2-Stratified CdS-Cu2-xS Core-Shell Nanorods for Highly Efficient Photocatalytic Hydrogen Production.ACS Nano. 2020, 14(5):5468-5479.
68.Wang Y, Jin R, Sojic N*, Jiang D*, Chen HY.Intracellular Wireless Analysis of Single Cells by Bipolar Electrochemiluminescence Confined in a Nanopipette.Angew Chem Int Ed Engl.2020, doi: 10.1002/anie..
67.Pan R, Hu K, Jia R, Rotenberg SA, Jiang D*, Mirkin MV*.Resistive-Pulse Sensing Inside Single Living Cells.J Am Chem Soc.2020, 142(12):5778-5784.
66. JinR, Huang YC, Cheng L, Lu HY, Jiang D*, Chen HY.In situ observation of heterogeneous charge distribution at the electrode unraveling the mechanism of electric field-enhanced electrochemical activity.Chem. Sci., 2020,11, 4158-4163
65.Cui C, Jin R, Jiang D*, Zhang J*, Zhu JJ*. Electrogenerated Chemiluminescence in Submicrometer Wells for Very High-Density Biosensing.Anal Chem.2020, 92(1):578-582.
64.Gao TB, Zhang JJ, Wen J, Yang XX, Ma HB, Cao DK*, Jiang D*. Single-Molecule MicroRNA Electrochemiluminescence Detection Using Cyclometalated Dinuclear Ir(III) Complex with Synergistic Effect.Anal Chem.2020, 92(1):1268-1275.
63. Li Y, Chen FZ, Xu YT, Li HY*, Fan GC, Han DM, Zhao WW*, Jiang D*Self-Assembled Peptide Nanostructures for Photoelectrochemical Bioanalysis Application: A Proof-of-Concept Study.Anal. Chem. 2019, 91, 20, 12606–12610
62. Pan R, Hu K, Jiang D*, Samuni U, Mirkin MV*. Electrochemical Resistive-Pulse Sensing.J Am Chem Soc.2019, 141(50):19555-19559.
61.Zhu H, Jin R, Jiang D*, Zhu JJ*. Perturbation Electrochemiluminescence Imaging to Observe the Fluctuation of Charge-Transfer Resistance in Individual Graphene Microsheets with Redox-Induced Defects.ACS Appl Mater Interfaces. 2019, 11(50):46666-46670.
60.Pan R, Jiang D.* Nanokits for the electrochemical quantification of enzyme activity in single living cells.Methods Enzymol.2019, 628:173-189.
59.Zhang J, Jin R, Jiang D*, Chen HY. Electrochemiluminescence-Based Capacitance Microscopy for Label-Free Imaging of Antigens on the Cellular Plasma Membrane.J Am Chem Soc.2019, 141(26):10294-10299.
58.Xu H, Yang D, Jiang D*, Chen HY. Phosphate Assay Kit in One Cell for Electrochemical Detection of Intracellular Phosphate Ions at Single Cells.Front Chem.2019, 7:360
57.Chen Y, Zhao D, Fu J, Gou X, Jiang D*, Dong H*, Zhu JJ*. In Situ Imaging Facet-Induced Spatial Heterogeneity of Electrocatalytic Reaction Activity at the Subparticle Level via Electrochemiluminescence Microscopy.Anal Chem. 2019, 91(10):6829-6835.
56.Zhang J, Arbault S, Sojic N*, Jiang D*. Electrochemiluminescence Imaging for Bioanalysis.Annu Rev Anal Chem2019, 12;12(1):275-295.
55.Zhang J, Ding H, Zhao SY, Jiang D*, Chen, HY. Confined Electrochemiluminescence in Vertically Ordered Silica Mesochannels for the Imaging of Hydrogen Peroxide Released from Single Cells.Electrochem. Commun. 2019, 98, 38-42.
54.Jin R, Ye X, Fan J, Jiang D*, Chen HY. In Situ Imaging of Photocatalytic Activity at Titanium Dioxide Nanotubes Using Scanning Ion Conductance Microscopy.Anal Chem. 2019, 91(4):2605-2609.
53.Huang S, Liu K, Jiang D, Fang D*. Codetermination of Sphingomyelin and Cholesterol in Cellular Plasma Membrane in Sphingomyelin-Depletion-Induced Cholesterol Efflux.Anal Chem. 2019, 91(2):1501-1506.
52.Cui C, Chen Y, Jiang D*, Chen HY, Zhang J*, Zhu JJ*. Steady-State Electrochemiluminescence at Single Semiconductive Titanium Dioxide Nanoparticles for Local Sensing of Single Cells.Anal Chem. 2019, 91(1):1121-1125.
51.Tao L, Qiao M, Jin R, Li Y, Xiao Z, Wang Y, Zhang N, Xie C, He Q, Jiang D, Yu G*, Li Y*, Wang S*. Bridging the Surface Charge and Catalytic Activity of a Defective Carbon Electrocatalyst.Angew Chem Int Ed Engl.2019, 58(4):1019-1024.
50.Xu M, Pan R, Zhu Y, Jiang D*, Chen HY*. Molecular profiling of single axons and dendrites in living neurons using electrosyringe-assisted electrospray mass spectrometry.Analyst.2019, 144(3):954-960.
49.Xu M, Pan R, Zhu Y, Jiang D*, Chen HY*. Resistive Analysis of Hydrogen Peroxide in One Axon of Single Neuron with Nanopipets. Anal Chem.2018, 90(17):10117-10121.
48 .Chen Y, Fu J, Cui C, Jiang D*, Chen Z*, Chen HY, Zhu JJ*. In Situ Visualization of Electrocatalytic Reaction Activity at Quantum Dots for Water Oxidation.Anal Chem.2018, 90(14):8635-8641.
47.Zhou J, Jiang D*, Chen HY. Nanoelectrochemical architectures for high-spatial-resolution single cell analysis. SCI China Chem.2018, 60, 1277-1284.
46.Zhou S, Peng X, Xu H, Qin Y, Jiang D*, Qu J*, Chen HY. Fluorescence Lifetime-Resolved Ion-Selective Nanospheres for Simultaneous Imaging of Calcium Ion in Mitochondria and Lysosomes. Anal Chem. 2018, 90(13):7982-7988.
45.Xia J, Zhou J, Zhang R, Jiang D, Jiang DP*. Gold-coated polydimethylsiloxane microwells for high-throughput electrochemiluminescence analysis of intracellular glucose at single cells.Anal Bioanal Chem. 2018, 410(20):4787-4792.
44.Pan R, Xu M, Burgess JD, Jiang D*, Chen HY. Direct electrochemical observation of glucosidase activity in isolated single lysosomes from a living cell.Proc Natl Acad Sci USA.2018, 115(16):4087-4092.
43.Gao TB, Zhang JJ, Yan RQ, Cao DK*, Jiang D*, Ye D*. Aggregation-Induced Electrochemiluminescence from a Cyclometalated Iridium(III) Complex.Inorg Chem.2018, 57(8):4310-4316.
42.Xu H, Zhou S, Jiang D*, Chen HY. Cholesterol Oxidase/Triton X-100 Parked Microelectrodes for the Detection of Cholesterol in Plasma Membrane at Single Cells.Anal Chem. 2018, 90(2):1054-1058.
41.Zhang J, Zhou J, Pan R, Jiang D*, Burgess JD, Chen HY. New Frontiers and Challenges for Single-Cell Electrochemical Analysis.ACS Sens. 2018, 3(2):242-250.
40. Li Y, Zhang N, Zhao WW*, Jiang D*, Xu JJ, Chen HY. Polymer Dots for Photoelectrochemical Bioanalysis.Anal Chem.2017;89(9):4945‐4950.
39.Zhang J, Zhou J, Tian C, Yang S, Jiang D*, Zhang XX*, Chen HY. Localized Electrochemiluminescence from Nanoneedle Electrodes for Very-High-Density Electrochemical Sensing.Anal Chem. 2017, 89(21):11399-11404.
38.Xu F, Zhou W, Cao J, Xu Q, Jiang D, Chen Y*. A Combination of DNA-peptide Probes and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS): A Quasi-Targeted Proteomics Approach for Multiplexed MicroRNA Quantification.Theranostics. 2017, 7(11):2849-2862.
37.Li L, Lu B, Jiang D, Shin M, Kelley T, Burgess JD*. Cell Plasma Membrane Cholesterol as a Diagnostic.Curr Opin Electrochem. 2017, 2(1):82-87.
36.Zhang X*, Han F, Syed A, Bukhari EM, Siang BCJ, Yang S, Zhou B, Wen WJ, Jiang D*. Fabrication of highly modulable fibrous 3D extracellular microenvironments.Biomed Microdevices. 2017, 19(3):53.
35.Cui C, Chen Y, Jiang D*, Zhu JJ*, Chen HY. Attomole Antigen Detection Using Self-Electrochemiluminous Graphene Oxide-Capped Au@L012 Nanocomposite.Anal Chem. 2017, 89(4):2418-2423.
34.Xu J, Jiang D, Qin Y, Xia J, Jiang D*, Chen HY. C3N4 Nanosheet Modified Microwell Array with Enhanced Electrochemiluminescence for Total Analysis of Cholesterol at Single Cells.Anal Chem. 2017, 89(4):2216-2220.
33.Pan R, Xu M, Jiang D*, Burgess JD, Chen HY. Nanokit for single-cell electrochemical analyses.Proc Natl Acad Sci USA.2016, 113(41):11436-11440.
32.Yang C, Qin Y, Jiang D*, Chen HY. Continuous Fluorescence Imaging of Intracellular Calcium by Use of Ion-Selective Nanospheres with Adjustable Spectra.ACS Appl Mater Interfaces. 2016, 8(31):19892-8.
31.Xu J, Huang P, Qin Y, Jiang D*, Chen HY. Analysis of Intracellular Glucose at Single Cells Using Electrochemiluminescence Imaging.Anal Chem. 2016, 88(9):4609-12.
30.Xu J, Zhai J, Xu Y, Zhu J, Qin Y*, Jiang D*. A near-infrared fluorescent aza-bodipy probe for dual-wavelength detection of hydrogen peroxide in living cells.Analyst.2016, 141(8):2380-3.
29.He R, Tang H, Jiang D*, Chen HY. Electrochemical Visualization of Intracellular Hydrogen Peroxide at Single Cells.Anal Chem. 2016, 88(4):2006-9.
28.Zhou J, Ma G, Chen Y, Fang D*, Jiang D*, Chen HY. Electrochemiluminescence imaging for parallel single-cell analysis of active membrane cholesterol.Anal Chem. 2015, 87(16):8138-43.
27.Liu Y, Qin Y*, Jiang D*. Squaramide-based tripodal ionophores for potentiometric sulfate-selective sensors with high selectivity.Analyst. 2015, 140(15):5317-23.
26.Liu Y, Zhu J, Xu Y, Qin Y*, Jiang D*. Boronic Acid Functionalized Aza-Bodipy (azaBDPBA) based Fluorescence Optodes for the Analysis of Glucose in Whole Blood.ACS Appl Mater Interfaces. 2015, 7(21):11141-5.
25.Zhuang L, Zuo H, Wu Z, Wang Y, Fang D*, Jiang D*. Enhanced electrochemical nanoring electrode for analysis of cytosol in single cells.Anal Chem. 2014, 86, 11517-22.
24.Han F, Jiang H, Fang D*, Jiang D*. Potential-resolved electrochemiluminescence for determination of two antigens at the cell surface.Anal Chem. 2014, 86, 6896-902.
23.Tian C, Zhou J, Wu ZQ, Fang D*, Jiang D*. Fast serial analysis of active cholesterol at the plasma membrane in single cells.Anal Chem.2014, 86, 678-84.
22.Ma G, Zhou J, Tian C, Jiang D*, Fang D*, Chen H. Luminol electrochemiluminescence for the analysis of active cholesterol at the plasma membrane in single mammalian cells.Anal Chem. 2013, 85, 3912-7.
21.Han F, Xu Y, Jiang D*, Qin Y*, Chen H. A bodipy-derived fluorescent probe for cellular pH measurements.Anal Biochem.2013, 435, 106-13.

20.Wang K, Jiang D, Sims CE, Allbritton NL*. Separation of fluorescently labeled phosphoinositides and sphingolipids by capillary electrophoresis.J Chromatogr B.2012, 15, 79-86.
19.Huang W, Jiang D, Wang X, Wang K, Sims CE, Allbritton NL*, Zhang Q*. Kinetic analysis of PI3K reactions with fluorescent PIP2 derivatives.Anal Bioanal Chem.2011,401,1881-8.
18.Jiang D, Sims CE, Allbritton NL*. Single-cell analysis of phosphoinositide 3-kinase and phosphatase and tensin homolog activation.Faraday Discuss.2011, 149, 187-200.
17.Jiang D, Sims CE, Allbritton NL*. Microelectrophoresis platform for fast serial analysis of single cells.Electrophoresis. 2010,31,2558-65.
16.Fang D, West RH, Manson ME, Ruddy J, Jiang D, Previs SF, Sonawane ND, Burgess JD, Kelley TJ*. Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesis.Respir Res.2010 May 20;11:61.
15.Fang D, Jiang D, Lu H, Chiel HJ, Kelley TJ, Burgess JD*. Observation of cellular cholesterol efflux at microcavity electrodes.J Am Chem Soc.2009, 131,12038-9.
14.Ding J, Jiang D, Kurczy M, Nalepka J, Dudley B, Merkel EI, Porter FD, Ewing AG, Winograd N, Burgess J, Molyneaux K*. Inhibition of HMG CoA reductase reveals an unexpected role for cholesterol during PGC migration in the mouse.BMC Dev Biol. 2008 Dec 31;8:120.
13.Jiang D, Fang D, Kelley TJ, Burgess JD*. Electrochemical analysis of cell plasma membrane cholesterol at the airway surface of mouse trachea.Anal Chem. 2008, 80, 1235-9.
12.Jiang D, Devadoss A, Palencsár MS, Fang D, White NM, Kelley TJ, Smith JD, Burgess JD*. Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells.J Am Chem Soc.2007,129, 11352-3.
11.White NM, Jiang D, Burgess JD, Bederman IR, Previs SF, Kelley TJ*. Altered cholesterol homeostasis in cultured and in vivo models of cystic fibrosis.Am J Physiol Lung Cell Mol Physiol. 2007, 292, L476-86.
10.Devadoss A, Palencsár MS, Jiang D, Honkonen ML, Burgess JD*. Enzyme modification of platinum microelectrodes for detection of cholesterol in vesicle lipid bilayer membranes.Anal Chem.2005, 77, 7393-8.
9.Jiang D, Tang J, Liu B, Yang P, Kong J*. Ultrathin alumina sol-gel-derived films: allowing direct detection of the liver fibrosis markers by capacitance measurement.Anal Chem.2003, 75, 4578-84.
8.Jiang D, Tang J, Liu B, Yang P, Shen X, Kong J*. Covalently coupling the antibody on an amine-self-assembled gold surface to probe hyaluronan-binding protein with capacitance measurement.Biosens Bioelectron.2003,18, 1183-91.
7.Xu X, Zhao J, Jiang D, Kong J, Liu B*, Deng J. TiO2 sol-gel derived amperometric biosensor for H₂O₂on the electropolymerized phenazine methosulfate modified electrode.Anal Bioanal Chem.2002,374, 1261-6.
6.Wang K, Jiang D, Liu B, Zhang S, Lu T, Kong J*. Label-free immunosensors-principles and applications.Chinese J Anal Chem, 2003, 33,411-416.
5.Liu Z, Huang S, Jiang D, Liu B, Kong J*.A novel capacitive immunosensor using electropolymerized insulating poly (o-phenylenediamine) film on a glass carbon electrode for probing transferrin.Anal Lett. 2003, 37, 2283-2301
4.Jiang D, Huang S, Tang J, Liu B, Huang Y, Kong J*. A high sensitive hyaluronan binding protein capacitive immunosensor.Chinese J Anal Chem, 2003, 31, 713-715.
3.Wang K, Jiang D, Kong J, Zhang S, Liu B*, Lu T*. Sensitively detecting recombinant hirudin variant-2 with capacitive immunoassay based on self-assembled monolayers.Anal Lett.2003, 36,2571-2583.
2.Yang H, Shi Q, Liu X, Xie S, Jiang D, Zhang F, Yu CZ, Tu B*. Synthesis of ordered mesoporous carbon monoliths with bicontinuous cubic pore structure of Ia3d symmetry.Chem. Comm.2002, 23, 2842-2843.
1.Zhang J*, Jiang D, Chen B, Zhu J, Jiang L, Fang H. Preparation and electrochemistry of hydrous ruthenium oxide/active carbon electrode materials for supercapacitor.J. Electrochem. Soc. 2001, 148,A1362-A1367.








课程名称、上课时间地点




教学大纲、考试要求




教学资源（上课讲义、参考资料等）





课题组风采


博士后：张晶晶、李玉、潘荣容


博士研究生: 王呢娜, 王玉玲, 金融

硕士研究生: 黄煜晨, 黄荣璨, 钱樱姿, 常昕骐