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南京大学化学化工学院导师教师师资介绍简介-许丹科

本站小编 Free考研考试/2021-02-15

职务:
联系电话:
办公地址: 南京大学仙林校区 化学化工学院
电子邮箱: xudanke@nju.edu.cn
http://hysz.nju.edu.cn/xudanke
课题组主页:





个人简介


许丹科博士,1987年南京大学化学系本科毕业;1997年获南京大学分析化学博士学位,师从陈洪渊院士;1987年至1992年在南京军区总医院工作,1997年至1999年在军事医学科学院从事博士后研究工作;2000至2007年为军事医学科学院副研究员、研究员,北京蛋白质组研究中心新技术研究室PI。2000-2002年作为访问****在美国加利福利亚大学河滨分校从事生物分析化学研究工作。2008年至今为南京大学化学化工学院教授,博士生导师,南京大学分析科学研究所副所长。主持了国家科技部973项目、国家基金委仪器专项等多个重点项目,已发表相关论文100余篇,参加编著国内外相关著作8本,获国家专利10余件。2012年获南京市领军型科技创业人才,现担任英国BMC Biotechnology杂志副主编,《中国测试》杂志编委,国家生物技术协会生物芯片专业委员会委员,中国生物医学工程学会医学检验工程分会委员,中国仪器仪表协会快检技术与仪器专业委员会委员。


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工作经历


1987年至1992年在南京军区总医院工作,1997年至1999年在军事医学科学院从事博士后研究工作;2000至2007年为军事医学科学院副研究员、研究员,北京蛋白质组研究中心新技术研究室PI。2000-2002年作为访问****在美国加利福利亚大学河滨分校从事生物分析化学研究工作。2008年至今为南京大学化学化工学院教授,博士生导师,南京大学分析科学研究所副所长。


展示全部

研究方向


1、生物分子识别;
2、微纳尺度生物分析;
3、检测及仪器装置



学术成果



2019年发表文章
1.Zhao, Y.; Tang, M.; Liu, F.; Li, H.; Wang, H.; Xu, D., Highly Integrated Microfluidic Chip Coupled to Mass Spectrometry for Online Analysis of Residual Quinolones in Milk. Anal.Chem.2019, 91 (21), 13418-13426
2.Jin, F.; Li, H.; Xu, D., Enzyme-free fluorescence microarray for determination of hepatitis B virus DNA based on silver nanoparticle aggregates-assisted signal amplification. Anal.Chim.Acta 2019, 1077, 297-304.
3.Yang, H.; Li, H.; Xu, D., High-density micro-well array with aptamer-silver conjugates for cell sorting and imaging at single cells. Anal.Chim.Acta2019, 1063, 127-135.
4.Yu, F.; Li, H.; Sun, W.; Xu, D.; He, F., Rapid selection of aptamers based on protein microarray. RSC Advances2019, 9 (17), 9762-9768.
5.Huang, A.; Li, H.; Xu, D., An on-chip electrochemical sensor by integrating ITO three-electrode with low-volume cell for on-line determination of trace Hg(II). Journal of Electroanalytical Chemistry2019, 848.
6. Xu M, Deng J, Xu K, Zhu T, Han L, Yan Y, Yao D, Deng H, Wang D, Sun Y, Chang C, Zhang X, Dai J, Yue L, Zhang Q, Cai X, Zhu Y, Duan H, Liu Y, Li D, Zhu Y, Radstake TRDJ, Balak DMW, Xu D, Guo T, Lu C, Yu X. In-depth serum proteomics reveals biomarkers of psoriasis severity and response to traditional Chinese medicine. Theranostics 2019; 9(9):2475-2488
7. Fang Yu, Hui Li*, Wei Sun, Yaju Zhao, Danke Xu, Fuchu He, Selection of aptamers against Lactoferrin based on silver enhanced and fluorescence-activate cell sorting, Talanta193 (2019) 110–117

2018年发表文章
1. Wenchao Jia, Zhongyi Lu, Hao Yang, Hui Li*, Danke Xu*, Elimination terminal fixed region screening and high-throughput kinetic determination of aptamer for lipocalin-1 by surface plasmon resonance imaging. Anal.Chim.Acta,2018, 1043, 158-166.
2. Yaju Zhao, Minmin Tang, Qiaobo Liao, Zhoumin Li, Hui Li,* Kai Xi, Li Tan, Mei Zhang, Danke Xu,* Hong-Yuan Chen, Disposable MoS2-arrayed MALDI MS chip for high-throughput and rapid quantification of sulfonamides in multiple real samples. ACS Sens., 2018, 3, 806-814.
3. Wenchao Jia, Hui Li, Thomas Wilkop, Xiaohui Liu, Xiaodong Yu, Quan Cheng, Danke Xu* and Hong-Yuan Chen, Silver Decahedral Nanoparticles Empowered SPR Imaging-SELEX for High Throughput Screening of Aptamers with Real-Time Assessment, Biosensors and Bioelectronics,2018, 109,206-213.
4. Zhu Chen, Hui Li*, Yaju Zhao, Meng Xu, Danke Xu*,Magnetic nanoparticles and polydopamine amplified FP aptasensor for the highly sensitive detection of rHuEPO-α,Talanta,2018, 189, 143-149.
5. Xiaohui Liu, Hui Li*, Yaju Zhao, Xiaodong Yu, Danke Xu*, Multivalent aptasensor array and silver aggregated amplification for multiplex detection in microfluidic devices, Talanta,2018,188,417-422.
2017年发表文章
1. Z. Chen, H. Li, W. Jia, X. Liu, Z. Li, F. Wen, N. Zheng, J. Jiang, D. Xu, Bivalent aptasensor based on silver-enhanced fluorescence polarization for rapid detection of lactoferrin in milk, Anal.Chem., 2017, 89(11), 5900?5908
2. Y. Zhao, D. Xu, W. Tan, Aptamer-functionalized nano/micro-materials for clinical diagnosis: isolation, release and bioanalysis of circulating tumor cells, Integrative Biology,2017,9, 188-205.
3. Xiaohui Liu, Hui Li*, Wenchao Jia, Zhu Chen, Danke Xu*, Selection of Aptamers Based on Protein Microarray Integrated with Microfluidic Chip.Lab on a chip, 2017,17,178-185
4. Hui Li, Yaju Zhao, Zhu Chen, Danke Xu*. Silver enhanced ratiometric nanosensor based on two adjustable Fluorescence Resonance Energy Transfer modes for quantitative protein sensing. Biosensors and Bioelectronics, 2017,87,428-432
5. Zhonghui Li, Zhoumin Li, Danke Xu*. Simultaneous detection of four nitrofuran metabolites in honey by using a visualized microarray screen assay. Food Chemistry, 2017,221,1813-1821
6. Zhonghui Li, Zhoumin Li, Jindou Jiang, Danke Xu*. Simultaneous detection of various contaminants in milk based on visualized microarray. Food Control,2017, 73, 994-1001
7. Zhoumin Li, Zhonghui Li, Dingyi Zhao, Fang Wen, Jindou Jiang, Danke Xu*. Smartphone-based visualized microarray detection for multiplexed harmful substances in milk. Biosensors and Bioelectronics,2017,87,874-880
8. Zhoumin Li, Zhonghui Li, Qiqi Niu, Hui Li, Maika Vuki, Danke Xu*. Visual microarray detection for human IgE based on silver nanoparticles. Sensors and Actuators B:Chemical, 2017,239,45-51
9. Zhoumin Li, Fang Wen, Zhonghui Li, Nan Zheng, Jindou Jiang and Danke Xu*, Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized Microarray. BMC Biotechnology. 2017,17:72-80.
10.Yu, X.; Petritis, B.; Duan, H.; Xu, D.; LaBaer, J., Advances in cell-free protein array methods. Expert Review of Proteomics2017, 15 (1), 1-11.

2016年发表文章
1. Ying Wang, Hui Li*, Danke Xu*. Aptamers-based sandwich assay for silver-enhanced fluorescence multiplex detection. Anal.Chim.Acta,2016,905,149-155
2. Wei Song, Qiqi Niu, Weibing Qiang, Hui Li*, Danke Xu*. Enzyme-free electrochemical aptasensor by using silver nanoparticles aggregates coupling with carbon nanotube inducing signal amplification through electrodeposition. Journal of Electroanalytical Chemistry,2016,781,62-69
3. Yaju Zhao, Xiaohui Liu, Jie Li, Weibing Qiang, Liang Sun, Hui Li, Danke Xu*. Microfluidic chip-based silver nanoparticles aptasensor for colorimetric detection of thrombin. Talanta. 2016,150,81-87
4. Yaju Zhao, Guoqing Deng, Xiaohui liu, Liang Sun, Hui Li, Quan Cheng, Kai Xi, Danke Xu*. MoS2/Ag nanohybrid: A novel matrix with synergistic effect for small molecule drugs analysis by negative-ion matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal.Chim.Acta, 2016,937,87-95

2015年发表文章
1. Hui Li, Hongting Hu, and Danke Xu*. Silver Decahedral Nanoparticles-Enhanced Fluorescence Resonance Energy Transfer Sensor for Specific Cell Imaging. Anal. Chem.2015,87,3826-3833
2. Hui Li, Hongting Hu, Yaju Zhao, Xiang Chen, Wei Li, Weibing Qiang, and Danke Xu*. Multifunctional Aptamer-Silver Conjugates as Theragnostic Agents for Specific Cancer Cell Therapy and Fluorescence-Enhanced Cell Imaging. Anal. Chem.2015,87,3736-3745
3. Wei Li, Yifan Dong, Xi Wang, Hui Li, Danke Xu*. PolyA-tailed and fluorophore-labeled aptamer-gold nanoparticle conjugate for fluorescence turn-on bioassay using iodide-induced ligand displacement. DOI:10.1016/j.bios.2014.10.047 Biosensors and Bioelectronics 66(2015)43-49
4. Weibing Qiang, Xi Wang, Wei Li, Xiang Chen,Hui Li, Danke Xu*. A fluorescent biosensing platform based on the polydopamine nanospheres intergrating with Exonuclease III-assisted target recycling amplification. DOI:10.1016/j.bios.2015.04.029 Biosensors and Bioelectronics2015,71,143-149
5. Weibing Qiang, Hongting Hu, Liang Sun, Hui Li, Danke Xu*. Aptamer/Polydopamine Nanospheres Nanocomplex for in Situ Molecular Sensing in Living Cells. Anal.Chem.2015,87,12190-12196
6. Xi Wang, Wei Li, Zhonghui Li, Hui Li*, Danke Xu*. A highly sensitive fluorescence turn-on platform with silver nanoparticles aptasening for human platelet-derived growth factor-BB. Talanta.2015,144,1273-1278
7. Li, R.; Shu, C.; Wang, W.; Wang, X.; Li, H.; Xu, D.; Zhong, W., Encapsulation of 10-Hydroxy Camptothecin in Supramolecular Hydrogel as an Injectable Drug Delivery System. Journal of Pharmaceutical Sciences2015, 104 (7), 2266-2275.

2014年发表文章
Hui Li, Danke Xu*. Silver nanoparticles as labels for applications in bioassays.TrAC2014. 61. 67-73.
Weibing Qiang, Wei Li, Xiaoqing Li, Xiang Chen, Danke Xu*. Bioinspired polydopamine nanospheres: a superquencher for fluorescence sensing of biomolecules. Chem. Sci., 2014, 5, 3018-3024.
Weibing Qiang, Haiping Liu, Wei Li, Xiang Chen, Danke Xu*. Label-free detection of adenosine energy transfer between fluore unmodified gold nanoparticles. Anal.Chim.Acta828 (2014) 92-98.
Wei Song, Hui Li, Hua Liang, Weibing Qiang, and Danke Xu*. Disposable Electrochemical Aptasensor Array by Using in Situ DNA Hybridization Inducing Silver Nanoparticles Aggregate for Signal Amplification, Anal. Chem., 2014, 86 , 2775–2783.
Hui Li, Min Wang, Weibing Qiang, Hongting Hu, Wei Li and Danke Xu*. Metal-enhanced fluorescent detection for protein microarrays based on a silver plasmonic substrate. Analyst, 2014, 139, 1653–1660 .
Hui Li, Yue Zhu, Shiyu Dong, Weibing Qiang, Liang Sun, Danke Xu*. Fast functionalization of silver decahedral nanoparticles with aptamers for colorimetric detection of human platelet-derived growth factor-BB. Anal.Chim.Acta, 2014,829,48-53.
Hongting Hu; Hui Li; Yaju Zhao; Shiyu Dong; Wei Li; Weibing Qiang; Danke Xu*. Aptamer-Functionalized Silver Nanoparticles for Scanometric Detection of Platelet-Derived Growth Factor-BB". Anal.Chim.Acta, 2014,812,152-160(IF=4.387)
Jie Li; Wei Li; Weibing Qiang; Xi Wang; Hui Li; Danke Xu*. A non-aggregation colorimetric assay for thrombin based on catalytic properties of silver nanoparticles".Anal.Chim.Acta, 807 (2014) 120- 125.
Wei Li, Weibing Qiang, Jie Li, Hui Li, Yifan Dong, Yaju Zhao, Danke Xu*. Nanoparticle-catalyzed reductive bleaching for fabricating turn-off and enzyme-free amplified colorimetric bioassays. Biosensors and Bioelectronics,2014, 51, 219–224.
10.Kourentzi, K.; Cao, B.; Hu, Y.; Duan, J.; Ma, J.; Xu, D.; Yang, X.-D., Selection of a Novel DNA Aptamer for Assay of Intracellular Interferon-Gamma. PLoS ONE2014, 9 (5).
2013年发表文章
Weibing Qiang, Hui Li and Danke Xu*.
Metal enhanced fluorescent biosensing assays for DNA through the coupling of silver nanoparticles Anal. Methods, 2013,5,629-635

Wei Li, Jie Li, Weibing Qiang, Jingjuan Xu and Danke Xu*
Enzyme-free colorimetric bioassay based on gold nanoparticle-catalyzed dye decolorization Analyst,2013, 138, 760-766

Haiping Liu, Ying Wang, Hui Li, Zhilin Wang, Danke Xu*
Luminescent Rhodamine B doped core-shell silica nanoparticle labels for protein microarray detection
Dyes and Pigments 98(2013)119-124

Wei Song, Hui Li, Haiping Liu, Zhishan Wu, Weibing Qiang, Danke Xu*
Fabrication of streptavidin functionalized silver nanoparticle decorated grapheme and its application in disposable electrochemical sensor for immunoglobulin Electrochemistry Communications31(2013)16-19

Hui Li, Min Wang, Chongzhi Wang, Wei Li, Weibing Qiang, Danke Xu*,
Silver nanoparticle-enhanced fluorescence resonance energy transfer sensor for human PDGF-BB detection. Anal. Chem.2013, 85,4492-4499.

Hui Li,Weibing Qiang,Chongzhi Wang, Maika Vuki,Danke Xu*,
Ultrasensitive and fast fluorescent bioassay based on fluorescence enhancement of silver nanoparticle. Analyst, 2013,138, 7376-7383.

2012年发表文章
Ying Wang, Zhonghui Li, Hui Li, Maika Vuki, Danke Xu*, Hong-Yuan Chen*.
A Novel Aptasensor Based on Silver Nanoparticle Enhanced Fluorescence.
Biosensors and Bioelectronics, 2012,32,76-81

Qing Chen, Zhoumin Li, Xirui Zhao, Xiaobo Yu,Maika Vuki, Wei He, and Danke Xu*.
A Highly-sensitive Colorimetric Assay Method for Antibody Array Based on an Tyramide Signal Amplification System.
Anal.Lett., 2012,45,219-226
Wang, Y.; Xu, D. K*.; Chen, H. Y.
Aptamer-based silver nanosensor for multiple protein detection.
Lab on a Chip2012, 12, 3184-3189.
Li, Z. H.; Yan, M.; Li, Z. M.; Vuki, M. K.; Wu, D.; Liu, F.; Zhong, W. Y.; Zhang, L. Y.; Xu, D. K*.
A multiplexed screening method for agonists and antagonists of the estrogen receptor protein. Anal.Bioanal.Chem.2012, 403, 1373-1384.

Li, H.; Chen, C. Y.; Wei, X.; Qiang, W. B.; Li, Z. H.; Cheng, Q.; Xu, D. K*.
Highly Sensitive Detection of Proteins Based on Metal-Enhanced Fluorescence with Novel Silver Nanostructures.
Anal.Chem.2012, 84, 8656-8662.

6. Xia Wei, Hui Li, Zhonghui Li, Maika Vuki , Yu Fan, Wenying Zhong*, Danke Xu*. Metal-enhanced Fluorescent Probes Based on Silver Nanoparticles and Its Application in IgE Detection.
Anal. Bioanal.Chem.,2012,402,1057-1063

2011年发表的文章:
Ziyin Sun, Weibing Qiang, Hui Li, Nan Hao, Danke Xu* , Hong-Yuan Chen*.
Electric detection of DNA with PDMS microgap electrodes and silver nanoparticles.
Analyst, 2011, 136, 540–544

Nan Hao, Hui Li, Yitao Long, Lei Zhang, Xirui Zhao, Danke Xu*, Hong-Yuan Chen*.
An electrochemical immunosensing method based on silver nanoparticles.
Journal of Electroanalytical Chemistry2011,656, 50–54

Hui Li, Weibing Qiang, Maika Vuki, Danke Xu,* and Hong-Yuan Chen.
Fluorescence Enhancement of Silver Nanoparticle Hybrid Probes and Ultrasensitive Detection of IgE.
Anal.Chem.2011,83,8945-8952





课程名称、上课时间地点



本科生授课课程:
《现代仪器分析》(课程编号**),化学生物学交叉班
《分子识别与分析》(课程编号**),高年级选修课

研究生授课课程:
1、《应用分析化学》,工程硕士课程


教学大纲、考试要求




教学资源(上课讲义、参考资料等)





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