Dr Wong Wing-leung Research Assistant Professor

Location Y820-C
Phone +852 3400 8871
mail wing.leung.wong@polyu.edu.hk

Open Platform for University Scholars
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Education and Academic Qualifications Doctor of Philosophy, City University of Hong Kong
Bachelor of Science in Chemical Technology, The Hong Kong Polytechnic University




Research Overview Our research focuses on the development of small-molecule fluorescent binding ligands that are able to function as biosensors and inhibitors targeting at the unique nucleic acid structures such as RNA and DNA G-quadruplexes and drug target proteins including telomerase, NDM-1, and peptidoglycan glycosyltransferase.




Research Interests
Organic Synthesis
Chemical Biology
Biosensing & Imaging
Catalysis
Ionic Liquid



Achievements

Awards and Patents
W.-L. Wong, L.-T. Leung, L. Zhang, C.-M. Chow, H. Zhu, L.-F. Lai, K.-K. Ho. Fullerene derivatives and their applications in organic photovoltaics. Granted US Patent, US9637384B2.
K.-K. Ho,W.-L. Wong. New conductive and photosensitive polymers. Granted US Patent, US9583711B2.
K.-Y. Wong, D. Ma,W.-L. Wong, W.-H. Chung, F.-Y. Chan. Luminescent protein staining and method for detecting poly(amino acid) in a mixture. Granted US Patent, US8318498B2.




Representative Publications
W. Long, B.-X. Zheng, X.-H. Huang, M.-T. She, A.-L. Liu, K. Zhang*,W.-L. Wong*, Y.-J. Lu*. Molecular Recognition and Imaging of Human Telomeric G-Quadruplex DNA in Live Cells: A Systematic Advancement of Thiazole Orange Scaffold To Enhance Binding Specificity and Inhibition of Gene Expression.Journal of Medicinal Chemistry, 2021, 64, 2125–2138.
B. Zheng, M.-T. She, W. Long, Y.-Y. Xu, Y.-H. Zhang, X.-H. Huang, W. Liu, J.-Q. Hou,W.-L. Wong*, Y.-J. Lu*. A small-sized benzothiazole-indolium fluorescent probe: the study of interaction specificity targeting c-MYC promoter G-quadruplex structures and live cell imaging.Chemical Communications, 2020, 56, 15016–15019.
J. Jin, J.-Q. Hou, W. Long, X. Zhang, Y.-J. Lu, D. Li, K. Zhang,W.-L. Wong*. Synthesis of fluorescent G-quadruplex DNA binding ligands for the comparison of terminal group effects in molecular interaction: phenol versus methoxybenzene.Bioorganic Chemistry, 2020, 99, 103821.
Y. Zhu, J.-Q. Hou, X.-H. Huang, D.-X. Zhong, W. Long, W. Liu, Y.-J. Lu, K. Zhang,W.-L. Wong*. Structural modification of nonspecific thiazole orange for ligand-DNA interaction study: understanding the ligand recognition selectivity towards G4-DNA over duplex-DNA.Journal of Luminescence, 2020, 226, 117488.
S. Hu, Y. Lin, J. Teng*,W.-L. Wong*, B. Qiu*. In situ deposition of MOF-74 (Cu) nanosheet arrays onto carbon cloth to fabricate a sensitive and selective electrocatalytic biosensor and its application for the determination of glucose in human serum.Microchimica Acta, 2020, 187, 670.
B-X. Zheng, W. Long, Y.-H. Zhang, X.-H. Huang, C.-C. Chen, D.-X. Zhong, M.-T. She, Z.-X. Chen, D.-P. Cai, Y.-J. Lu*,W.-L. Wong*. Rational design of red fluorescent and selective G-quadruplex DNA sensing probes: the study of interaction signaling and the molecular structural relationship achieving high specificity.Sensors and Actuators: B. Chemical, 2020, 314, 128075.
C. Wang, Y.-J. Lu*, S.-Y. Cai, W. Long, Y.-Y. Zheng, J.-W. Lin, Y. Yan, X.-H. Huang,W.-L. Wong*, K. Zhang. Advancing small ligands targeting RNA for better binding affinity and specificity: a study of structural influence through molecular design approach.Sensors and Actuators B: Chemical, 2018, 262, 386–394.
N. Sun, C. Wang, M.-H. Xu, Y.-J. Lu*, Y.-Y. Zheng, Y. Yan, X. L. Guo, J.-Q. Hou, K. Zhang, L. G. Luyt,W.-L. Wong*. The interaction of a structural flexible small molecule with nucleic acid structures: investigation of the origin of fluorescence signal discrimination in sensing and the utilization in live cell imaging.Sensors and Actuators B: Chemical, 2017, 250, 543–551.
Y.-J. Lu*, D.-P. Hu, K. Zhang,W.-L. Wong*, C. Chow. New pyridinium-based fluorescent dyes: a comparison of symmetry and side-group effects on G-quadruplex DNA binding selectivity and application in live cell imaging.Biosensors and Bioelectronics, 2016, 81, 373–381.
Y.-J. Lu*, Q. Deng, J.-Q. Hou, D.-P. Hu, Z.-Y. Wang, K. Zhang, L. G. Luyt,W.-L. Wong*. Molecular engineering of thiazole orange dye: change of fluorescent signaling from universal to specific upon binding with nucleic acids in bioassay.ACS Chemical Biology, 2016, 11, 1019–1029.