Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 夏天小姐 (病理解剖及细胞学系) |
| 刘建盟博士 (病理解剖及细胞学系) |
| 吴浩强教授 (病理解剖及细胞学系) |
| 李嘉慧博士 (药剂学院) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1371/journal.pone.0127910 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/3WOS source URL
其它资讯
摘要Many facets of the tumor biology of medulloblastoma (MB) have not been fully elucidated. Collapsin response mediator protein 1 (CRMP1) is a member of cytoplasmic family of proteins that regulate the development of central nervous system. Recent studies demonstrated that CRMP1 could function as an invasion suppressor. We reported previously that high mobility group AT-hook 1 (HMGA1) contributed to development of MB and regulated its growth and migration/invasion. Transcriptional profiling and quantitative RT-PCR revealed increased expression of CRMP1 in HMGA1-depleted cells, suggesting that CRMP1 may be a downstream target of HMGA1 in MB. In this study, we showed HMGA1 can bind CRMP1 promoter by chromatin immunoprecipitation (ChIP) assay. Luciferase assay demonstrated a marked enhancement of CRMP1 transcription activity in HMGA1-depleted cells. Furthermore, quantitative RT-PCR revealed a negative correlation between HMGA1 and CRMP1 in http://aims.cuhk.edu.hk/converis/portal/Publication/32 MB samples. To investigate the biological roles of CRMP1 in MB pathogenesis, we established MB clones stably expressing CRMP1. Functional analysis revealed that expression of CRMP1 significantly inhibited proliferation, migration, invasion and formation of filopodia and intense stress fiber of MB cells. Our data suggest that HMGA1 regulates CRMP1 expression and CRMP1 is implicated in MB pathogenesis.
着者Li KKW, Qi Y, Xia T, Yao Y, Zhou LF, Lau KM, Ng HK
期刊名称PLoS ONE
出版年份2015
日期26
卷号10
期次5
出版社PUBLIC LIBRARY SCIENCE
国际标準期刊号19http://aims.cuhk.edu.hk/converis/portal/Publication/32-620http://aims.cuhk.edu.hk/converis/portal/Publication/3
语言英式英语
Web of Science 学科类别Multidisciplinary Sciences; Science & Technology - Other Topics
