Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 韦妙宜教授 (那打素护理学院) |
| 李卓予教授 (生物医学学院) |
| 冯国培教授 (生物医学学院) |
| 徐国荣教授 (生物医学学院) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/0167-4781(95)00224-3 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/14WOS source URL
其它资讯
摘要During the large scale partial sequencing of human heart cDNA clones, a novel clone which is very similar to the rat ribosomal protein L29 in both DNA and amino acid sequences was found. The cDNA encodes a protein with a deduced molecular weight of 17 751 (159 aa). It shows 80.4% homology to protein L29 from the large ribosomal subunit of rat and is related to yeast YL43. The putative protein was named human ribosomal protein L29 (hRPL29). hRPL29 has a large excess of basic residues over acidic ones. The large amount of charged residues makes the protein very hydrophilic and the protein has a deduced pi of 12.16. Internal repeats have been characterised in many ribosomal proteins and a tandem repeat of KAKAKAKA was found to be unique to hRPL29. Analysis of gene organisation by Southern blotting shows that of the approximate 10 copies of hrpL29, all but one are pseudogenes. Northern analysis indicated that the mRNA that encodes human L29 is approx. 800 base pairs in length. An intron of hrpL29 has also been cloned and sequenced by polymerase chain reaction using human genomic DNA as the template.
着者Law PTW, Tsui SKW, Lam WY, Luk SCW, Hwang DM, Liew CC, Lee CY, Fung KP, Waye MMY
期刊名称BBA - Gene Structure and Expression
出版年份1996
月份3
日期1
卷号1305
期次3
出版社ELSEVIER SCIENCE BV
页次105 - 108
国际标準期刊号0167-4781
语言英式英语
关键词(human); cDNA; ribosomal protein L29; sequence comparison
Web of Science 学科类别Biochemistry & Molecular Biology; Biophysics
