Isolation and characterization of CHO cell mutants with altered asparagine synthetase.
Publication in refereed journal


香港中文大学研究人员 ( 现职)

韦妙宜教授 (那打素护理学院)

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典藏于此文库的全文 Somatic Cell Genetics Waye and Stanners http://aims.cuhk.edu.hk/converis/portal/Publication/1979.pdf 数位物件识别号 (DOI) http://dx.doi.org/10.1007/BF01542699 出版商提供的全文 http://link.springer.com/search?query=Somatic+Cell+and+Molecular+Genetics

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摘要Two asparagine auxotrophic mutants (N3, N4) were isolated from the Gat- strain of Chinese hamster ovary cells, using a selection procedure modified from that of Goldfarb et al. (1). The defect in these mutants is due to a deficiency in asparagine synthetase activity. N3, in particular, had no measurable enzyme activity. Complementation analysis by PEG-mediated cell fusion showed that the auxotrophic phenotype behaved as a recessive trait; complementation was obtained between N3 or N4 and the pseudoauxotroph, Asn3, which has a temperature-sensitive asparagyl-tRNA synthetase activity. Revertants obtained by plating N3 or N4 in asparagine-free medium had about normal levels of asparagine synthetase activity and were produced with a probability of about 10(-6) per cell per generation. Three particular revertants of N3 and one revertant of N4 were shown to have asparagine synthetase activities that were different in thermolability from that of the wild type. This observation is consistent with the suggestion that N3 and N4 have defective structural genes rather than defective regulatory genes for asparagine synthetase.

出版社接受日期01.08.http://aims.cuhk.edu.hk/converis/portal/Publication/1979
着者Waye MM, Stanners CP
期刊名称Somatic Cell and Molecular Genetics
出版年份http://aims.cuhk.edu.hk/converis/portal/Publication/1979
月份9
日期1
卷号5
期次5
出版社Springer Verlag (Germany)
出版地USA
页次625 - 639
国际标準期刊号0740-7750
语言美式英语

关键词CHO cell mutants, asparagine synthetase, somatic cell genetics