Publication in refereed journal
香港中文大学研究人员 ( 现职)
| Professor Malcolm John UNDERWOOD (外科学系) |
| 刘建盟博士 (病理解剖及细胞学系) |
| 何名熙教授 (麻醉及深切治疗学系) |
| 万松教授 (外科学系) |
| 吴士衡教授 (外科学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/j.ejcts.2010.01.001 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/6WOS source URL
其它资讯
摘要Objectives: Pulmonary dysfunction following lung ischaemia-reperfusion is a well-known phenomenon, which may contribute to post-cardiac surgical morbidity. The process is associated with pulmonary inflammatory response and cellular apoptosis. Early molecular mechanisms leading to such lung injury remain largely unknown. We examined whether lung ischaemia and reperfusion cause significant expression changes in numerous genes in the lungs involved in pulmonary apoptosis and other cellular processes by using oligonucleotide microarrays in an experimental model of rodent lung ischaemia reperfusion injury. Methods: Sprague-Dawley rodents (n = 5 in each group) were anaesthetised and underwent controlled ventilation, with varying durations of warm lung ischaemia (http://aims.cuhk.edu.hk/converis/portal/Publication/60 and 90 min) followed by a short reperfusion period. The right middle lobe of the lung was harvested. Gene expression changes in the lungs were analysed by rodent DNA microarray chips, and reverse transcription polymerase chain reaction (RT-PCR) performed to validate changes in gene expression. Results: Significant expression changes, with reference to false discovery rate (FDR) controls, were detected in over 80 genes following controlled lung ventilation, and more than 50 were up-regulated more than 2-fold. Lung ischaemia reperfusion caused expression changes in over 50 additional genes, including many novel genes not previously associated with lung ischaemia reperfusion. Up-regulated genes identified include those associated with apoptosis, inflammation and cell-cycle control. Conclusions: Large numbers of genes relating to cell metabolism, transcription control, inflammation and apoptosis were significantly up- and down-regulated following controlled ventilation and early lung ischaemia reperfusion, consistent with previous studies. In addition, novel genes related to lung injury were identified. These genetic signatures provide new insights into early molecular mechanisms of ischaemia reperfusion lung injury and help refine therapeutic strategies to lessen pulmonary dysfunction following cardiac surgery. (C) 2010 European Association for Cardio-Thoracic Surgery. Published by Elsevier B.V. All rights reserved.
着者Ng CSH, Hui CWC, Wan S, Wan IYP, Ho AMH, Lau KM, Darzi AW, Underwood MJ
期刊名称European Journal of Cardio-Thoracic Surgery
出版年份2010
月份http://aims.cuhk.edu.hk/converis/portal/Publication/6
日期1
卷号37
期次http://aims.cuhk.edu.hk/converis/portal/Publication/6
出版社ELSEVIER SCIENCE BV
页次1411 - 1420
国际标準期刊号1010-7940
电子国际标準期刊号1873-734X
语言英式英语
关键词Apoptosis; Ischaemia-reperfusion; Ventilation
Web of Science 学科类别Cardiac & Cardiovascular Systems; CARDIAC & CARDIOVASCULAR SYSTEMS; Cardiovascular System & Cardiology; Respiratory System; RESPIRATORY SYSTEM; Surgery; SURGERY
