Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 罗国炜教授 (病理解剖及细胞学系) |
| 吴浩强教授 (病理解剖及细胞学系) |
| 许碧瑜博士 (病理解剖及细胞学系) |
| 梁韵姿小姐 |
| 唐婉君女士 (病理解剖及细胞学系) |
| 蔡光伟教授 (妇产科学系) |
| 周捷博士 (病理解剖及细胞学系) |
| 杜家辉教授 (病理解剖及细胞学系) |
| 锺天恩博士 (化学病理学系) |
| 柯欣欣博士 (病理解剖及细胞学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1002/path.2609 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/20WOS source URL
其它资讯
摘要Nasopharyngeal carcinoma (NPC) is a distinct type of head and neck cancer commonly occurring in southern China. To decipher the molecular basis of this cancer, we performed high-resolution array CGH analysis on eight tumour lines and 10 primary tumours to identify the genes involved in NPC tumorigenesis. In this study, multiple regions of gain were consistently found at 1q21-q24, 7q11-12, 7q21-22., 11q13, 12p13, 12q13, 19p13 and 19q13. Importantly, a 2.1 Mb region at 12p13.31 was highly amplified in a NPC xenograft, xeno-2117. By FISH mapping, we have further delineated the amplicon to a 1.24 region flanked by RP11-319E16 and RP11-433J6. Copy number gains of this amplicon were confirmed in 21/41 (51%) primary tumours, while three cases (7.3%) showed high copy number amplification. Among the 13 genes within this amplicon, three candidate genes, lymphotoxin beta receptor (LT beta R), tumour necrosis factor receptor superfamily memeber 1A (TNFRSF1R) and FLJI0665, were specifically over-expressed in the NPC xenograft with 12p13.3 amplification. However, only LT beta R was frequently over-expressed in primary tumours. LT beta R is a member of the TNF family of receptors, which can modulate NF-kappa B signalling pathways. Over-expression of LT beta R in nasopharyngeal epithelial cells resulted in an increase of NF-kappa B activity and cell proliferation. In vivo study showed that suppression of LT beta R by siRNA led to growth inhibition in the NPC tumour with 12p13.3 amplification. These findings implied that LT beta R is a potential NPC-associated oncogene within the 12p13.3 amplicon and that its alteration is important in NPC tumorigenesis. Copyright (C) http://aims.cuhk.edu.hk/converis/portal/Publication/2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
着者Or YYY, Chung GTY, To KF, Chow C, Choy KW, Tong CYK, Leung AWC, Hui ABY, Tsao SW, Ng HK, Yip TTC, Busson P, Lo KW
期刊名称Journal of Pathology
出版年份http://aims.cuhk.edu.hk/converis/portal/Publication/2010
月份1
日期1
卷号2http://aims.cuhk.edu.hk/converis/portal/Publication/20
期次1
出版社JOHN WILEY & SONS LTD
页次97 - 107
国际标準期刊号0022-3417
电子国际标準期刊号1096-9896
语言英式英语
关键词12p13.3 amplicon; array CGH; Epstein-Barr virus; LT beta R; nasopharyngeal carcinoma; oncogene
Web of Science 学科类别Oncology; ONCOLOGY; Pathology; PATHOLOGY
