袁宝银，男，副教授（校聘）
研究方向：基于核酸适配体的肿瘤检测与治疗
地址：郑州市科学大道100号郑州大学 基础医学院 病理生理学系
邮箱：yuanbaoyin@zzu.edu.cn
学习、工作经历 ：
2019.1-至今郑州大学，基础医学院，病理生理学系，直聘副教授
2018.7-2018.12 郑州大学，基础医学院，病理生理学系，讲师
2014.9-2018.6 湖南大学，化学化工学院，化学，博士
2012.9-2014.6 湖南大学，生物学院，生物学，硕士
2008.9-2012.6 周口师范学院，生命科学系，生物技术，学士
教学承担：
《病理生理学》本科课程、《Pathophysiology》留学生课程、《高级病理生理学》研究生课程
研究方向与特色：
核酸适配体（Aptamer）是一类由指数富集配体系统进化技术(SELEX)筛选得到的短单链DNA或RNA核苷酸序列，能够特异识别肿瘤细胞及相关肿瘤标志物。除了具备与抗体类似的识别性能外，Aptamer的很多特性优于抗体，如易合成、易修饰、性质稳定、设计灵活、无免疫原性和生物毒性等，被誉为“化学抗体”，是一种极具潜力的新型肿瘤识别分子，已在肿瘤的分子诊断、靶向治疗及相关基础研究中显示出独特优势和重要应用价值。本人目前主要开展肿瘤细胞核酸适配体的筛选和性能研究、基于核酸适配体的肿瘤早期检测和治疗以及肿瘤细胞表面糖基化成像等工作。
主持项目：
1.河南省重点研发与推广专项（科技攻关），**0031，基于核酸适配体-DNA的荧光激活式策略用于肿瘤RTK糖基化检测，2020/01-2021/12，10万，主持。
2.国家自然科学基金重点项目，**，基于RNA疾病标志物的精准诊疗研究，2018/01-2022/12，320万，参与。
3.国家自然科学基金面上项目，**，基于核酸适配体的新型肿瘤多药耐药逆转剂的研究和应用，2019/01-2022/12，65万，参与。
发表文章：
1.BaoyinYuan, Yuqiong Sun, Qiuping Guo,* JinHuang, Xiaohai Yang, Yuanyuan Chen, Xiaohong Wen, Xiangxian Meng, Jianbo Liu,Kemin Wang*.High signal-to-background ratio detection of cancer cells withactivatable strategy based on target-induced self-assembly of split aptamers. Anal. Chem., 2017, 89, 9347−9353.
2.Baoyin Yuan, Yuanyuan Chen, Yuqiong Sun, Qiuping Guo,* Jin Huang,Jianbo Liu, Xiangxian Meng, Xiaohai Yang, Xiaohong Wen, Zenghui Li, Lie Li,Kemin Wang.* Enhanced imaging of specific cell-surface glycosylation based onmulti-FRET. Anal.Chem.,
3.BaoyinYuan,‡Yu Zhou,‡ Qiuping Guo,* Kemin Wang,* Xiaohai Yang, Xiangxian Meng,Jun Wan, Yuyu Tan, Zhixiang Huang, Qin Xie and Xiayu Zhao. A signal-on split aptasensorfor highly sensitive and specific detection of tumor cells based on FRET.
4.BaoyinYuan,‡
5.Xiaohong Wen‡,Baoyin Yuan‡, Junxun Zhang, Xiangxian Meng, Qiuping Guo,* LieLi, Zenghui Li, Huishan Jiang and Kemin Wang*. Enhancedvisualization of cell surface glycans via a hybridization chain reaction. Chem.Commun., 2019, 55, 6114—6117.
6.Baoyin Yuan,Linyan Guo, Kai Yin, Xinyu Wang, Qian Liu, Meimei He, Kangdong Liu,* JiminZhao.* Highly sensitive and specific detection of tumor cells based on a splitaptamer-triggered dual hybridization chain reaction. Analyst, 2020, 145,2676-2681.
7.Zenghui Li,‡Baoyin Yuan,‡ Xiaoxia Lin, Xiangxian Meng, Xiaohong Wen,Qiuping Guo,∗,Lie Li, Huishan Jiang, Kemin Wang.∗Intramolecular trigger remodeling-induced HCR for amplified detection ofprotein-specific glycosylation. Talanta, 2020, 215, 120889.
8.Yuqiong Sun,‡Baoyin Yuan,‡ MeitaoDeng, Qing Wang, Jin Huang, Qiuping Guo, Jianbo Liu, Xiaohai Yang,* KeminWang.* A light-up fluorescence assay for tumor cell detection based onbifunctional split aptamers. Analyst,2018, 143 (15), 3579-3585.
9.Yuyu Tan,‡Qiuping Guo,‡ Qin Xie, Kemin Wang,* Baoyin Yuan, Yu Zhou, Jianbo Liu, Jin Huang, Xiaoxiao He, XiaohaiYang, Chunmei He, Xiayu Zhao. Single-walled carbon nanotubes (SWCNTs)-assistedcell-systematic evolution of ligands by exponential enrichment (Cell-SELEX) forimproving screening efficiency. Anal.Chem., 2014, 86, 9466-9472.
10.Taiping Qing,Xiaoxiao He,* Dinggeng He, Xiaosheng Ye,Jingfang Shangguan, Jinquan Liu, BaoyinYuan, Kemin Wang.* Dumbbell DNA-templatedCuNPs as a nano-fluorescent probe for detection of enzymes involved inligase-mediated DNA repair. Biosens. Bioelectron.,2017, 94, 456-46.
11.JunWan,‡ Ling Ye,‡ Xiaohai Yang, Qiuping Guo,* Kemin Wang,*Zhixiang Huang, Yuyu Tan, Baoyin Yuan,Qin Xie. Cell-SELEX based selection and optimization of DNA aptamers forspecific recognition of human cholangiocarcinoma QBC-939 cells.
12.QinXie,‡ Yuyu Tan,‡ Qiuping Guo,* Kemin Wang,*
13.ZhixiangHuang, Qin Xie, Qiuping Guo*, Kemin Wang*, Xiangxian Meng, Baoyin Yuan, Jun Wan, Yuanyuan Chen. DNA aptamer selected forspecific recognition of prostate cancer cells and clinical tissues.
14.QiupingGuo, Xiayu Zhao, Qin Xie, Kemin Wang,* Jun Wan, Baoyin Yuan, Yuyu Tan. Label-free fluorescence signal amplifyingdetection of DNA based on hairpin probe and Exo III. Chem. J Chinese U, 2014,35, 1646-1651.
