降解水源水中有机物的优势菌筛选及其特性

张多英, 李伟光, 刘苗



作者单位

张多英  

李伟光  

刘苗  



摘要:

为解决活性炭生物增强技术(BEAC)应用过程中接种高活性菌群稳定性差的问题,必须分离出既具有有机物降解能力,又具有种群竞争能力的菌株.应用变性梯度聚丙烯酰胺凝胶电泳(DGGE)技术,分析BAC滤池优势菌群结构,结果表明,有4种细菌占优势地位,其中2种可降解有机污染物,分别为Pseudomonas sp.和Bacillus subtilis.而从长期运行的普通生物活性炭(BAC)滤池中共分离出35株细菌.针对BAC滤池中的优势菌设计探针,应用荧光原位杂交(FISH)技术快速鉴别优势菌.结果分离菌株中仅5株细菌的种属与优势菌一致,分别为SRO2施氏假单胞菌(Pseudomonas stutzeri)、SRO11恶臭假单胞菌(Pseudomonas putida)、SRO19穿孔假单胞菌(Pseudomonas pertucinogena)、SRO20巴利阿里假单胞菌(Pseudomonas balearica)、SRO30枯草芽孢杆菌(Bacillus subtilis).有机物降解试验表明,该5株菌均具有有机物降解能力.测定脱氢酶活性以确定优势菌的生物活性,结果表明,该5株菌的脱氢酶活性高于其他菌株.可为BEAC长期稳定运行提供菌种资源.

关键词:  活性炭生物增强技术  优势菌  变性梯度凝胶电泳  荧光原位杂交  脱氢酶活性

DOI：10.11918/j.issn.0367-6234.2011.08.010

分类号:X172

基金项目:国家自然科学基金资助项目(51078106);黑龙江省杰出青年基金资助项目(JC200708);黑龙江省教育厅青年学术骨干支持计划项目(1251G049)



Isolation and characterization of bacteria with biodegradability of organic pollutants in surface water

ZHANG Duo-ying, LI Wei-guang, LIU Miao

Abstract:

During bioenhanced activated carbon(BEAC)process,the inoculated bacterial communities are dynamic.For solving this problem,bacteria with biodegradability and competitive power must be isolated.DGGE profiles of BAC filter indicated that there were 4 dominant bacteria,and 2 of them could degrade organ-ic pollutants,which were Pseudomonas sp.and Bacillus subtilis.Thirty five bacteria were isolated from BAC filter using LB medium.Target probe of Pseudomonas sp.and Bacillus subtilis were designed for testing domi-nant bacteria quickly.After fluorescence in situ hybridization(FISH),5 dominant bacteria were identified and they were SRO 2(Pseudomonas stutzeri),SRO 11(Pseudomonas putida),SRO 19(Pseudomonas pertucinogena),SRO 20(Pseudomonas balearica)and SRO 30(Bacillus subtilis),respectively.The biodegradation test of organic pollutants in Songhuajiang water showed that the 5 bacteria could degrade organic pollutants.Dehydrogenase activities in these 5 bacteria were higher than other isolates,so that these five bac-teria were valuable bacterial resource for applying in BEAC process.

Key words:  bioenhanced activated carbon  dominant bacteria  DGGE  FISH  dehydrogenase activity