姓名：陈同生
学历：博士
职称：教授（博士生导师）
职务：院长
研究方向：生物医学光子学、细胞生物学
联系电话：**
电子邮箱：chentsh@scnu.edu.cn
通讯地址：广东省广州市华南师范大学激光生命科学教育部重点实验室（510631）
个人主页：http://biop.scnu.edu.cn/teacherview.asp?id=104

个人简历
发表论文
出版著作
科研项目
主要获奖
会议论文
个人空间


特别信息：招收物理学、光学工程、生物学和化学专业的硕士和博士研究生以及博士后，欢迎联系（chentsh@scnu.edu.cn; **），具体内容详见其个人空间。


《关于做好2019年“广东省博士后人才引进计划”申报工作的通知》
http://rsc.scnu.edu.cn/a/**/684.html



《华南师范大学生物光子学研究院陈同生课题组招聘启事》(2020-1-30）
http://biop.scnu.edu.cn/a/**/311.html


简介：

1964年4月出生，华南师范大学生物光子学研究院院长、学术委员会主任，激光生命科学教育部重点实验室教授，博士生导师，广东省“千百十人才工程”省级培养对象。主持国家863项目1项（230万）、国家重大科研仪器研制项目1项（510万）、国家自然科学基金面上项目5项，主持广东科技计划项目1项、重点项目1项以及其他省部级项目4项。研究领域主要涉及生物医学光子学、生物物理学、肿瘤细胞生物学、分子生物学。发表学术论文140余篇，获得授权国家发明专利5项。
教育简历：
1982.9-1986.6：湖北师范学院物理专业毕业，获理学学士学位；
1990.9-1993.5：华中科技大学光电子与物理电子专业毕业，获理学硕士学位；
1999.9-2003.5：华中科技大学生物医学工程专业毕业，获工学博士学位；
工作简历：
1986.6-1990.9：湖北师范学院物理系教师，助教；
1993.9-2000.9：湖北师范学院物理系教师，讲师；
2000.9-2003.11：华中科技大学生命科学院，副教授；
2003.11-2005.12：华南师范大学，副教授, 副所长；
2005.12-2013: 华南师范大学激光生命科学教育部重点实验室 教授，博士导师；
2013-2018.06: 华南师范大学生物光子学研究院学术委员会主任，教授，博导；
2018.07-至今: 华南师范大学生物光子学研究院院长、学术委员会主任，教授，博导；


陈同生教授主要成果清单（*通讯作者）
2016年
1 Tan Li, Liping Wu, Jiang Zhang, Gaina Xi, Yilin Pang, Xiaoping Wang and Tongsheng Chen*. Hydrothermal Reduction of Polyethylenimine and Polyethylene Glycol Dual-Functionalized NanoGraphene Oxide for high-efficiency Gene Delivery. ACS Appl. Mater. Interfaces 2016, 8, 31311?31320.
2 Tan Li, Hongyu Liu, Gaina Xi, Yiling Pang, Liping Wu, Xiaoping Wang ^*, Tongsheng Chen*. One step reduction and PEIylation ofPEGylated nanographene oxide for high-efficient chemo-photothermal therapy. J Mater Chem B 2016,4: 2972 – 2983.
3 Yingyao Quan, GuiqiQin, Hao Huang, Yuhong Liu, Xiaoping Wang, Tongsheng Chen*. Dominant roles of Fenton reaction in sodium nitroprusside-induced chondrocyte apoptosis. Free Radical Biology & Medicine 2016, 94: 135-144.
4 Mengyan Du, Lili Zhang, Shusen Xie, Tongsheng Chen*. Wide-field microscopic FRET imaging using simultaneous spectral unmixing of excitation and emission spectra. Opt. Express2016, 24(14), 16037-16051.
5 aina Xi, Xiaoping Wang, Tongsheng Chen*. A reduced graphene oxide-based FRET sensor for highly sensitive detection of matrix metalloproteinase 2. Int J Nanomed 2016, 11:1537–1547
6 Jiang Zhang, Fangrui Lin, Liuying Chai, Lichun Wei, Tongsheng Chen*. IIem-spFRET: Improved Iem-spFRET method for robust FRET measurement. J Biomed Opt 2016, 21(10), 105003
7 Yilin Pang, Guiqi Qin, Liping Wu, Xiaoping Wang, Tongsheng Chen*. Artesunate induces ROS-dependent apoptosis via a Bax-mediated intrinsic pathway in Huh-7 and Hep3B cells. Experimental Cell Research 2016, 347: 251–260
8 Hongyu Liu, Tan Li, Yuhong Liu, Guiqi Qin, Xiaoping Wang, and Tongsheng Chen*. Glucose-reduced Graphene Oxide with Excellent Biocompatibility and Photothermal Efficiency as well as Drug Loading. Nanoscale Res Lett 2016, 11:211.
9 Jiang Zhang, Lili Zhang, Liuying Chai, Fangfang Yang, Mengyan Du, Tongsheng Chen*. Reliable measurement of the FRET sensitized-quenching transition factor for FRET quantification in living cells. Micron 2016, 88:7-15.
10 ZP Wang, YLi,Q Zhou, YF Wang, TS Chen. Berberine-loaded solid lipid nanoparticles enhance hypoglycemic efficacy on streptozotocin-induced diabetic C57BL/6 mice. Nanomed-Nanotechnol Biol Med 2016,12(2):483-484.
11 XL Li and TS Chen. Enhancement and suppression effects of a nanopatterned surface on bacterial adhesion. PHYSICAL REVIEW E 2016(93), 052419.
12 H Huang, YY Quan, XP Wang, TS Chen. Gold Nanoparticles of Diameter 13 nm Induce Apoptosis in Rabbit Articular Chondrocytes. Nanoscale Res Lett 2016, 11:249.


2015年
1 Lili Zhang, Guiqi Qin, Liuying Chai, Jiang Zhang, Fangfang Yang, Hongqin Yang, Shusen Xie,Tongsheng Chen*. Spectral wide-field microscopic FRET imaging in live cells.J. Biomed. Opt. 2015,20(8), 086011
2 Guiqi Qin, Hongyu Liu, Liping Wu, Yilin Pang, Chubiao Zhao, Shengnan Wu,Xiaoping Wang,Tongsheng Chen*.Artesunate inducesapoptosis via a ROS-independentBax-mediated intrinsic pathway in HepG2 cells. Exp Cell Res 2015, 336(2):308-317
3 Guiqi Qin, Lili Zhang, Hongyu Liu, Yingyao Quan, Liuying Chai, Shengnan Wu, Xiaoping Wang,Tongsheng Chen*. Dihydroartemisinin induces apoptosis preferentially via a Bim/Bak-mediated intrinsic pathway in hepatocarcinoma cells. Apoptosis 2015, 20:1072–1086.
4 Liuying Chai, Jianwei Zhang, Lili Zhang,Tongsheng Chen*.Miniature fiber optic spectrometer-based quantitative fluorescence resonance energy transfer measurement in single living cells," J. Biomed. Opt. 20(3), 037008 (2015).
5 Bingling Chen, Zhouyi Guo,Tongsheng Chen*.Maximum Entropy Method-based Acceptor Triplet-State Fluorescence Correlation Spectroscopy Analysis for Determination of Donor-Acceptor Distance Distribution: Theory and Simulation. IEEE Photonics Journal 2015，7(3):**,DOI 10.1109/JPHOT.2015.**
6 Jianwei Zhang, Huali Li, Liuying Chai, Lili Zhang, JunLe Qu,Tongsheng Chen*.Quantitative FRET measurement via unmixing emission spectra with independent cross excitation correction. J Microscopy 2015, 257(2):104–116.
7 Jianwei Zhang, Fangfang Yang, Liuying Chai, Lili Zhang, Junle Qu,Tongsheng Chen*.Spectral measurement of acceptor-to-donor extinction coefficient ratio in living cells. Micron 68 (2015) 98–106.
8 Zhiping Wang, Junbiao Wu, Qun Zhou, Yifei Wang, andTongsheng Chen. Berberine Nanosuspension Enhances Hypoglycemic Efficacy on Streptozotocin Induced Diabetic C57BL/6 Mice. Evidence-Based Complementary and Alternative Medicine 2015, 239749.
2014年
1 陈同生，于怀娜。一种FRET效率定量检测方法。国家发明专利（2014）专利号：ZL.8
2 Jingqin Chen,Hongyu Liu, Chubiao Zhao, Guiqi Qin, Gaina Xi, Tan Li,Xiaoping Wang,andTongsheng Chen*.One-step Reduction and PEGylation of Graphene Oxide for Photothermally Controlled Drug Delivery. Biomaterials2014, 35：4986-4995，DOI 10.1016/j.biomaterials.2014.02.032.
3 Lili Zhang, Huaina Yu, Jianwei Zhang, Xiaoping Wang,Tongsheng Chen*.Binomial distribution-based quantitative measurement of multiple-acceptors fluorescence resonance energy transfer by partially photobleaching acceptor.APPLIED PHYSICS LETTERS104, 243706 (2014).
4 Chubiao Zhao, Guiqi Qin, Weijie Gao,Tongsheng Chen*. Dihydroartemisinin more intensely induces apoptosis in gemcitabine-resistant A549 cells via enhancing Bax-mediated intrinsic pathway. Cell Signal 2014，26(10): 2223-2233. DOI 10.1016/j.cellsig.2014.07.001
5 Chubiao Zhao, Weijie Gao,Tongsheng Chen*.Synergistic induction of apoptosis in A549 cells by dihydroartemisinin and gemcitabine. Apoptosis (2014) 19:668–681, DOI: 10.1007/s10495-013-0953-0
6 Jingqing Chen, Xiaoping Wang,Tongsheng Chen*.Facile and green reduction of covalently PEGylated nano-graphene oxide via a “water-only” route for high-efficiency photothermal therapy. Nanoscale Res Lett. 2014,9:86,DOI: 10.1186/1556-276X-9-86.
7 Qian Liang, Xiao-ping Wang,Tong-sheng Chen.Resveratrol protects rabbit articular chondrocyte against sodium nitroprusside-induced apoptosis via scavenging ROS.Apoptosis 2014,2014,19 (9):1354-1363.DOI 10.1007/s10495-014-1012-1.
8 Hua Jin, Qian Liang,Tongsheng Chen,XiaoPing Wang. Resveratrol protects chondrocytes from apoptosis via altering the ultrastructural and biomechanical properties: an AFM study. PLOS ONE 2014, 9(3): e91611, DOI 10.1371/journal.pone.**.
2013年
9 Weijie Gao, Fenglian Xiao, Xiaoping Wang, Tongsheng Chen*. Artemisinin induces A549 cell apoptosis dominantly via a reactive oxygen species-mediated amplification activation loop among caspase-9, -8 and -3. Apoptosis 2013,18(10): 1201-1213
10 Jing Qi, Yonghong Shao, Lixin Liu, Kaige Wang,Tongsheng Chen*, Junle Qu,* and Hanben Niu.Fast flexible multiphoton fluorescence lifetime imaging using acousto-optic deflector. Optical Letter 2013, 38(9):1697-1699
11 Tongsheng Chen*,Min Chen, Jingqin Chen. Dihydroartemisinin induces apoptosis and sensitizes cells to low-dose ionizing radiation largely by a ROS-mediated extrinsic apoptosis pathway in A549 cells. Plos One 2013, 8(3): e59827. DOI: 10.1371/journal.pone.**
12 Huaina Yu, Jianwei Zhang, Huali Li, andTongsheng Chen*. Ma-PbFRET: Multiple Acceptors FRET Measurement Based on PartialAcceptorPhotobleaching. Microscopy and Microanalysis2013，19(1):171-179,.
13 Caiping Zhuang, Xiaoping Wang, andTongsheng Chen*.H2o2 Induces Apoptosis Of Rabbit Chondrocytes Via Both The Extrinsic and The Caspase-Independent Intrinsic Pathways. Journal of Innovative Optical Health Sciences, 2013, 6( 3): **
14 YingYao Quan, Chaoyang Wang, XiaoPing Wang,Tongsheng Chen.Taxol induces cell death with cytoplasm vacuolization in paraptosis-like but not oncosis fashion in ASTC-a-1 cells.Journal of Innovative Optical Health Sciences,2013
2012年
15 Huali Li, Huaina Yu,Tongsheng Chen*.Quantitative measurement of FRET efficiencyfree from spectral bleed-throughin living cells. Microsc Microanal 2012，18: 1021–1029.
16 Huaina Yu, Jianwei Zhang, Huali, Li Junle Qu,Tongsheng Chen*. An empirical quantitative FRET method for multiple acceptors based on partial acceptor photobleaching. Appl Phys Letter, 2012，100, 253701
17 WeiWei Zhang, XiaoPing Wang,Tongsheng Chen*. Resveratrol induces apoptosis via a Bak-mediated intrinsic pathway in human lung adenocarcinoma cells. Cellular Signaling 2012，24(5):1037-1046
18 Chenjun Zhou, Wenliang Pan,Tongsheng Chen*. Artesunate induces apoptosis largely via a Bak-mediated caspases-independent intrinsic pathway in human lung adenocarcinoma cells. J Cell Physiol 2012，227(12):3778-3786
19 Fenglian Xiao, Weijie Gao, Xiaoping Wang,Tongsheng Chen*. Amplification activation loop between caspase-8 and -9 dominates artemisinin-induced apoptosis of ASTC-a-1 cells. Apoptosis 2012，17(6): 600-611.
20 Chaoyang Wang,Tongsheng Chen*. Intratumoral injection of taxol in vivo suppresses A549 tumor showing cytoplasmic vacuolization. J Cell Biochem 2012, 113:1397–1406
21 Min Chen,Tong-sheng Chen*, Ying-ying Lu, Cheng-yi Liu, Jun-le Qu. Dihydroarteminsin (DHA) induced apoptosis is not dependent on the translocation of Bim to the endoplasmic reticulum in human lung adenocarcinoma cells. Pathol. Oncol. Res. (2012) 18:809–816
22 Haiyang Li, Xiaoping Wang,Tongsheng Chen*.P38 Inhibitor SB203580 Sensitizes the Resveratrol-Induced Apoptosis in Human Lung Adenocarcinoma (A549) Cells.Journal of Biochemical and Molecular Toxicology2012, 26(7): 251
23 XiaoPing Wang，JianWei Zhang, Huai-na Yu,Tong-sheng Chen*.Quantitatively monitoringthe caspase-3 activationin single live cells using partial acceptor photobleachingFRET method.Journal of Innovative Optical Health Sciences(JHOS) 2012，5(3),**
24 陈同生，于怀娜.一种基于受体部分光漂白和供体-受体交替激发的FRET效率定量检测方法.国家发明专利，专利申请号：.4
2011年
25 Lei Sun,Tongsheng Chen*, Xiaoping Wang, Yun Chen, Xunbin Wei. Bufalin Induces Reactive Oxygen Species (ROS)-Dependent Bax Translocation and apoptosis in ASTC-a-1 Cells. EVID-BASED COMPL ALT 2011, 249090
26 Wenjing Guo, Lei Qian, Jing Zhang, Wei Zhang,Tongsheng Chen*, and Jie Zhao. SIRT1 overexpression in neurons promotes neurite outgrowth and cell survival with reduced mTOR signaling. J Neurosci Res 2011，89(11)：1723-1736
27 Weiwei Zhang, Xiaoping Wang,Tongsheng Chen*.Resveratrol induces mitochondria-mediated AIF- and to a lesser extent caspase-9-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells. Molecular and Cellular Biochemistry 2011，354:29–37DOI: 10.1007/s11010-011-0802-9
28 WeiWei Zhang, Zhi-Ping Wang,Tongsheng Chen*. Curcumol induces apoptosis via caspases-independent mitochondrial pathway in huaman lung adenocarcinoma ASTC-a-1 cells. MEDICAL ONCOLOGY 2011, 28(1):307-314
29 Fenglian Xiao,Tongsheng Chen*,Chengyi Liu, Xiaoping Wang. Artemisinin induces caspase-8/9-mediated and Bax/Bak-independent apoptosis in human lung adenocarcinoma (ASTC-a-1) cells. Journal of X-Ray Science and Technology（JXST） 2011，19(4): 545-555.
2010年
30 Li-hui Wang, Jin Xiang, Min Yan, Yan Zhang, Yan Zhao, Cai-feng Yue, Jie Xu, Fei-meng Zheng, Jin-na Chen, Zhuang Kang,Tong-sheng Chen, Da Xing, and Quentin Liu. Mitotic kinase Aurora-A induces mammary cell migration and breast cancer metastasis through up-regulating cofilin activity. Cancer Research, 2010，70(22):9118-9128
31 陈同生，王龙祥.基于光漂白的荧光共振能量效率的定量测量方法.国家发明专利，专利号：ZL4.9
32 Qing Rui Sun,Tong Sheng Chen*,Xiao Ping Wang, Xunbin Wei. Taxol induces paraptosis independent of protein synthesis and MAPK pathway. J Cell Physiol 2010, 222( 2) : 421-432
33 Ying-Ying Lu,Tong-Sheng Chen*, Xiao-Ping Wang, Jun-Le Qu. The JNK inhibitor SP600125 enhances dihydroartemisinin-induced apoptosis by accelerating Bax translocation into mitochondria in human lung adenocarcinoma cells. FEBS Letter, 584 (2010)：4019–4026（SCI IF 3.6）
34 Ying-Ying Lu,Tong-Sheng Chen*, Xiao-Ping Wang , Li Li. Single cell analysis of dihydroartemisinin(DHA)-induced apoptosis through reactive oxygen species (ROS)-mediated caspase-8 activation and mitochondrial pathway in ASTC-a-1 cells using fluorescence imaging techniques.J. Biomed. Opt., 2010,15(4): 046028-1-16
35 Min Chen, Ying-ying Lu,Tong-sheng Chen*，Jun-le Qu. Confocal Imaging of Bim Translocation to ER during DHA-Induced ASTC-a-1 Cell Apoptosis. Chinese Optical Letter, 2010, 8(10):950-953
36 Huai-na Yu,Tong-sheng Chen*, Jun-le Q. Improving FRET efficiency measurement in confocal microscopy imaging. Chinese Optical Letter, 2010, 8(10)：947-949
37 Longxiang Wang,Tongsheng Chen*, Junle Qu, Xunbin Wei. Photobleaching-Based Quantitative Analysis of Fluorescence Resonance Energy Transfer Inside Single Living Cell. J Fluorescence 2010, 20(1):27-35.
38 Wenjing Guo,Tongsheng Chen*, Xiaoping Wang, Rong Chen. Taxol induces concentration-dependent cell death form in ASTC-a-1 cells.Journal of X-Ray Science and Technology (JXST) , 18 (2010) 293–308
2009年
39 Longxiang Wang，Tongsheng Chen*,JunLe Qu et al. Quantitative analysis of caspase-3 activation by fitting fluorescence emission spectra in living cells. Micron 2009, 40(12): 811–820
40 Ying-Ying Lu,Tong-Sheng Chen*,Jun-Le Qu, Wen-Liang Pan, Lei Sun, Xunbin Wei.Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma cells. J Biomed Sci2009，16(1):16-. doi:10.1186/1423-0127-16-16
41 Wenliang Pan, Junle Qu,Tongsheng Chen*,Lei Sun, Jing Qi.FLIM and emission spectral analysis of caspase-3 activation inside single living cell during anticancer drug-induced cell death. Eur Biophy J 2009，38:447–456
42 Longxiang Wang, Xiangbo Yang,Tongsheng Chen.Second harmonic generation in generalized Thue–Morse ferroelectric superlattices. Physica B: Condensed Matter,2009, 404: 3425-3430
43 Xiaoping Wang, Dongmei He, Jiye Cai,Tongsheng Chen, Feiyan Zou, Yalan Li ,Yangzhe Wu, Zheng W. Chen, Yong Chen. WGA-QD probe-based AFM detects WGA-binding sites on cell surface and WGA-induced rigidity alternation. Biochem. Biophys. Res. Commun.**：335–340
2008年：
44 Tong-sheng Chen*, Xiao-ping Wang, Lei Sun, Long-xiang Wang, Da Xing，Martin Mok．Taxol induces caspase-independent cytoplasmic vacuolization and cell death through endoplasmic reticulum (ER) swelling in ASTC-a-1 cell. Cancer Letters 270 (2008)：164-172.
45 Lei Liu, Da Xing, Wei R. Chen,Tongsheng Chen, Yihui Pei, and Xuejuan Gao．Calpain-mediated pathway dominates cisplatin-induced apoptosis as determined by real-time single cell analysis. International Journal of Cancer. 122, 2210-2222 (2008)..
46 Xiao-Ping Wang,Tong-Sheng Chen*, Lei Sun, Ji-Ye Cai, Ming-Qian Wu,Martin Mok．Live Morphological Analysis of Taxol-Induced Cytoplasmic Vacuoliazation in human lung adenocarcinoma cells. Micron 2008，39：1216-1221．
47 Lei Sun, Xiao-Ping Wang,Tong-Sheng Chen*．Involvement of Bax and caspase-3 activation in Chan-Su-induced ASTC-a-1 cell apoptosis. Journal of X-ray Science and Technology (JXST) (2008), 16:131–142．
48 CHEN Tong-sheng*, WANG Xiao-ping, Sun Lei, WANG Hui-ying, WANG Long-xiang．Caspase-3 is Involved in the Chan-Su(CS)-induced Human LungAdenocarcinoma(ASTC-a-1) Cell Apoptosis. Progress in Biochemistry and Biophysics, 2008, 35(1):83-90．
49 CHEN Tong-sheng*, WANG Xiao-ping, WANG Zhi-ping, WANG Long-xiang1, WANG Hui-ying. Fluorescence Emission Analysis of Caspase-3 Activation Induced by Xiao-Ai-Ping (XAP) Inside Living Human Lung Adenocarcinoma Cells. Spec & Spec Anal.2008，28（6）: 1327-1331．
50 CHEN Tong-sheng*, WANG Xiao-ping, SUN Lei, WANG Hui-ying, WANG Long-xiang. Fluorescence Analysis of Taxol-Induced Paraptosis-Like Independent of Caspase-3 Activation. Spec & Spec Anal2008，28(11)：2623-2627．
51 Xiao-ping Wang,Tongsheng Chen*, Longxiang Wang, Lei Sun. Live Imaging of Xiao-Ai-Ping-induced Cell Death in Human Lung Adenocarcinoma Cells. Journal of Innovation in Optical Health Science (JIOHS) 2008, 1(1): 151-156．
2007年之前
52 Tongsheng Chen, Jinjun Wang, Da Xing and Wei R. Chen．Spatio-temporal dynamic analysis of Bid activation and apoptosis induced by alkaline condition in human lung adenocarcinoma cell. Cell physiology and biochemistry．Cell Physiol Biochem，2007，20(8)：569-578
53 Yihui pei, Da xing, Xuejuan gao,Tongsheng chen, Lei liu, Real-time Monitoring Full length Bid Interacting with Bax during TNF-α-induced Apoptosis, 2007, Apoptosis, 12:1681-1690
54 Yin-Yuan WU, Da XING, Lei LIU,Tong-sheng CHEN,and Wei CHEN. FRET Analysis of Bid Activation in Living Cells during UV-induced Apoptosis. Acta Biochimica et Bophysica，2007，39(1):37-45
55 MiaoJuan Chen, Da Xing,Tongsheng Chen, and Lan Zhang. JNK phosphorylation of BimL Induces Bax activation during UV irradiation-induced apoptosis. BBRC, 2007, 352(2), 559-565
56 Shengnan Wu, Da Xing, Fang Wang,Tongsheng Chen, Wei R. Chen, Mechanistic Study of Apoptosis Induced by High Fluence Low Power Laser Irradiation Using Fluorescence Imaging Techniques, J. Biomed. Opt., 12, (2007),12(6), 064015.
57 Xuejuan Gao#,Tongsheng Chen#, et al. Single Cell Analysis of PKC Activation During Proliferation and Apoptosis Induced by Laser Irradiation. J Cell Physiol., 2006, 206：441－448 (#并列第一作者)
58 Fang Wang#,Tong-Sheng Chen#,et al.Measuring Dynamics of Caspase-3 Activity in Living Cells Using FRET Technique During Apoptosis Induced by High Fluence Low Power Laser Irradiation. Laser in Surgery and Medicine 2005，36：2－7
59 Fang WANG,Tong-Sheng CHEN, Da XING. Measuring dynamics of caspase-3 activation in living cell by FRET during high dose low energy laser irradiation-induced cell apoptosis. SPIE, 2005, 5630：593（
60 陈同生，曾绍群，骆清铭.基于调Q脉冲激光的多光子激发荧光方法.国家发明专利，2004，专利号：ZL **.9
61 曾绍群，陈同生，骆清铭.实时定量测量荧光共振能量转移效率的方法.国家发明专利，2004，专利号：ZL OO114550.9
62 TongSheng Chen，Shaoqun Zeng, Qingming Luo et al., A Quantitative Theory Model of Photobleaching Mechanism. Chin. Phys. Lett. 2003，20(11):1940-1943
63 陈同生,曾绍群，骆清铭等，供体受体双光子激发吸收截面比值的测量,自然科学进展, 2003，13(12)：1312-1315
64 TongSheng Chen，Shaoqun Zeng, Qingming Luo et.al. High-Order Photobleaching of Green Fluorescent Protein inside live Cells in Two-Photon Excitation Microscopy. Biochemical and biophysical research communications, 2002，291(5): 1272-1275


主持承担的主要基金项目
1. 国家重大科研仪器研制项目（主持）：定量FRET显微成像仪的研制(**)，研究总经费510.32万元，直接经费442.1万元，起止年月：2016.01-2020.12
2. 国家自然科学基金面上项目（主持）：基于线性光谱分离的活细胞实时定量FRET显微成像技术研究(**) 80万，起止年月：2015.01-2018.12
3. 广东省科技计划项目（主持）：活细胞FRET光谱定量检测仪的研制(2014B)，100万,起止年月：2015.01-2018.12
4. 广州市科技计划项目（主持）：光谱FRET定量检测分析技术研究（2014J**）, 8万元，起止年月：2014.04-2016.04
5. 国家自然科学基金面上项目（主持）：活细胞中蛋白质寡聚化动态过程实时荧光成像与定量分析技术研究(**)，78万元，起止年月：2012.1-2015.12.
6. 国家自然科学基金面上项目（主持）：显微光学成像术研究紫杉醇诱导肿瘤细胞内质网空泡化和类副凋亡的分子调控机理(**)，35万元，起止年月：2011.1-2013.12.
7. 广东省高教厅、财政厅重点项目（主持）：非小细胞肺癌对吉非替尼耐药分子机制的单细胞可视化研究(cxzd115)，25万元，起止年月：2011.10-2014.10。
8. 国家自然科学基金面上项目（主持）：荧光技术在活细胞中实时研究中药诱导肿瘤细胞凋亡的分子机理(**)，28万元，起止年月：2007.1-2009.12。

（1）组织光学成像理论若干关键问题研究，2003年度湖北省自然科学二等奖，骆清铭，曾绍群，朱丹，吴龟灵，陈同生。
（2）光学与生命科学交叉科学中的前沿问题与关键技术研究.2009年度广东省科学技术奖（自然科学类）二等奖，邢达，朱德斌，陈同生，章春笋 张玲瑞，刘镭，唐永红等。

会议论文：(*通讯作者)
1Hai-yang Li, Cai-ping Zhuang, Xiao-ping Wang,Tong-sheng Chen*. SB203580 enhances the RV-induced loss of mitochondrial membrane potential and apoptosis in A549 cells. Proc. of SPIE 2012, 8224, 82240L(EI收录)
2Chao-yang Wang,Tong-sheng Chen*.Taxol-induced paraptosis-like A549 cell death is not senescence. Proc. of SPIE 2011, 7900: 79000K-1（EI收录）
3Huai-na Yu, Ying-ying Lu,Tong-sheng Chen*. Bad is not involved in DHA-induced apoptosis in human lung adenocarcinoma ASTC-a-1 cells. Proc. of SPIE 2011, 7900: 79000J-1（EI收录）
4Wen-jing Guo,Tong-sheng Chen*. Taxol induces concentration-dependent phosphatidylserine (PS) externalization and cell cycle arrest in ASTC-a-1 cells. Proceedings of the SPIE, 7565：75650J-75650J-7 (2010). DOI: 10.1117/12.839552 （EI收录）
5Feng-lian Xiao,Tong-sheng Chen*, Jun-Le Qu, Cheng-Yi Liu. Artemisinin induces ROS-mediated caspase3 activation in ASTC-a-1 cells. Proceedings of the SPIE, Volume 7565, pp. 75650I-75650I-7 (2010)，DOI: 10.1117/12.839551(EI收录）
6Wei-wei Zhang, Zhi-ping Wang,Tong-sheng Chen*.Bax is not involved in the resveratrol-induced apoptosis in human lung adenocarcinoma cells. Proceedings of the SPIE, Volume 7565, pp. 75650K-75650K-7 (2010)，DOI: 10.1117/12.839631. (EI收录）
7Ying-Ying Lu,Tong-Sheng Chen*，Jun-Le Qu. Bax translocation into mitochondria during dihydroartemisinin(DHA)-induced apoptosis in human lung adenocarcinoma cells. Proc. of SPIE2009，7178, 71780J
8Wen-liang Pan,Tong-sheng Chen*,Jun-Le Qu. Emission spectral analysis of caspase-3 activation during artesunate-induced apoptosis of human lung adenocarcinoma cell. Proc. of SPIE2009，7178, 71780I
9Qingrui Sun,Tongsheng Chen*.Reactive oxygen species (ROS) is not a promotor of taxol-induced cytoplasmic vacuolization. Proc. of SPIE 2009，7178, 71780K
10Tong-sheng Chen *, Lei Sun, Huiying Wang, Longxiang Wang．Fluorescence Imaging Analysis of Taxol-Induced Cell Death with Cell Swelling and Cytoplasmic Vacuolization．Proceedings of the SPIE 2008, 6857, 68570L-68570L-8 (2008) (EI收录)．
11Lei Sun,Tongsheng Chen*, Longxiang Wang, Huiying Wang．Analysis of caspase3 activation in ChanSu-induced Apoptosis of ASTC-a-1 Cells by Fluorescence Techniques．Proceedings of the SPIE 2008, 6857, 68570I-68570I-8 (2008) (EI收录)．
12Huiying Wang,Tongsheng Chen*,Lei Sun．Analysis of caspase-3 activation in mitomycin C-induced ASTC-a-1 cell apoptosis using acceptor photobleaching techniques．Proceedings of the SPIE 2008, 6857, pp. 68570J-68570J-7 (2008) (EI收录)．
13Tongsheng Chen, et al. Dynamic imaging of interaction between proteins 14-3-3and Bid in living cells, SPIE, 2006, 6087:60870L(EI收录)
14Tongsheng Chen, et al. Real-time single cell analysis of molecular mechanism of apoptosis and preliforaion using FRET technique, SPIE, 2006, 6047:604736(EI收录)
15Tongsheng Chen, Da Xing. Application of the FRET for Monitoring the Dynamics of Caspase-3 Activation during Apoptosis in Living Cells. SPIE, 2005, 5630: 368（EI收录）
16Tongsheng Chen, Da Xing. Application of the FRET for Monitoring the Dynamics of Caspase-3 Activation during Apoptosis in Living Cells. SPIE, 2004, 5630:368(EI收录)
17Tongsheng Chen, Shaoqun Zeng, Qingming Luo et al., High-Order Photobleaching in Two-Photon Excitation FluorescenceMicroscopy Inside Live Cell. SPIE. 2003 . 4967: 63-67（EI收录）
18Tongsheng Chen, Yafeng Liu, Shaoqun Zeng, Qingming Luo. Photobleaching of fluorophores at lower concentration under two-photon excitation microscopy. SPIE, 2003，5254:508-512（EI收录）
19Tongsheng Chen,Shaoqun Zeng, Qingming Luo. Interaction way of photobleaching produced bythe interaction between excitation photons and fluorophores molecules in excited states. SPIE,2003，4967:185-190（EI收录）
20Tongsheng Chen,et. al. Data analysis in green fluorescent protein based resonance energy transfer. SPIE. 2000, 4224: 346-350(EI收录)。

陈同生教授招聘博士后研究人员启事
2015-07-29
陈同生，华南师范大学****、广东省千百十省级培养对象、生物光子学研究院学术委员会主席。陈同生教授研究小组目前承担国家重大科研仪器、国家自然科学基金和广东省科技计划等科研项目6项，在研项目研究经费950余万元。现需招收光电、物理、生物学、自动控制以及化学方面的博士后。
一、招聘条件及要求
1、专业基础良好，具有较强的理论推导和编程能力，拥有相关研究经历，学术业绩优良；
2、有事业心和良好的科研工作背景；
3、其余招聘条件按《华南师范大学博士后科研人员进站申请指南》执行，详细流程和材料要求请见本网站“服务指南”中的《华南师范大学博士后科研人员出站申请指南》。
二、福利待遇
1、享受华南师范大学博士后相关待遇，详情请见《华南师范大学博士后科研人员进站申请指南》；
2、此外，每年生活补贴和奖金3-8万。
三、研究方向和课题
1、显微荧光成像技术研究；
2、光学器件的研制；
3、细胞信号转导分子机制的研究；
4、生物材料及其应用研究。
四、应聘材料
应聘者应提供相关材料：简历、近照、已发表论文、导师和相关领域专家推荐信2封、工作设想或其它应聘者认为重要的材料。应聘材料可通过邮寄或电子邮件方式提交。
在合作导师和学院考核遴选通过后，需按照《华南师范大学博士后科研人员进站申请指南》的要求向学校提交申请材料，逐级审批。
五、联系方式
通讯地址：广东省广州市天河区华南师范大学生物光子学研究院（收件人：陈同生，邮编：510631）
Email:chentsh@scnu.edu.cnandchentsh126@126.com
联系电话：**
（一）荧光共振能量转移(FRET)技术简介
FRET（Fluorescence/Ferster resonance energy transfer）是一个依赖于供体和受体分子间距离的光物理进程，处于激发态的荧光团通过偶极子间的相互作用将能量以非辐射的方式转移给邻近的受体分子，从而导致供体荧光淬灭和受体荧光发射的增加。供体和受体之间的能量转移效率 (E) 与分子间的空间距离 (r) 满足6次方的关系：

现代生物学的研究不仅需要了解参与生物过程的分子种类，更需要了解生物学过程的精确分子调控机制。尽管体外和体内研究已经揭示了大量特异的生物分子之间的相互作用，但大量发生于生物体内动态的或瞬时性的或者弱的分子间相互作用目前还难以准确鉴定和深入研究。
荧光共振能量转移(FRET)显微成像技术是目前唯一(unique)可以在单个活细胞中直接实时高时空分辨检测分子间动态相互作用过程的成像技术。^。基于荧光蛋白(FPs)FPs的FRET显微成像技术（FPs-FRET）已经成为在单个活细胞中研究分子间动态相互作用的最主要技术手段。
（二）本课题组开展对外服务
本课题组发展了多种单个活细胞中FRET效率的定量检测技术，获得多项国家发明专利，目前可开展多种形式的对外合作服务。
1. 合作形式：
（1） 独立承担单个活细胞中FRET定量检测分析；
（2） 联合申报国家和省部级重大科研项目。
2. 研究问题类型：
(1) 在单个活细胞中研究蛋白质间相互作用；
(2) 在单个活细胞中研究蛋白质寡聚化；
(3) 在单个活细胞中检测信号转导分子间的动态调控过程；
(4) 在单个活细胞中研究蛋白激酶的活性；
(5) 在单个活细胞中研究蛋白质的磷酸化；
(6) 在单个活细胞中研究生物大分子的构象变化。