袁吉锋YUAN Jifeng, Ph.D.
教授，博士生导师
闽江********
合成生物学、代谢工程
Synthetic Biology and Metabolic Engineering
课题组长
E-mail：jfyuan@xmu.edu.cn
西安交通大学学士（2007）；
新加坡南洋理工大学博士（2012）；
新加坡国立大学，研究科学家（2012-2016）；
新加坡科技研究局，高级研究员（2016-2017）；
新加坡国立大学，研究员（2017-2018）；
2018年至今，厦门大学生命科学学院，闽江********。
B.Eng., Xi’an Jiaotong University (2007);
Ph.D., Nanyang Technological University(2012);
Research Scientist, National University of Singapore (2012-2016);
Senior Research Fellow, Agency for Science, Technology and Research (2016-2017);
Research Fellow, National University of Singapore (2017-2018);
Professor, Xiamen University(2018).
研究领域
关键词：合成生物学、代谢工程、基因组编辑、生物催化与生物转化、手性不对称合成、天然产物与药物合成。
本课题组致力于研究绿色、经济、可持续的生物催化方式，以可再生的生物质为原料生产高附加值药物中间体、香精香料、精细化学品、天然产物等。通过合成生物学技术、代谢工程手段改造和设计高效细胞工厂用于生产各类高附加值产品。具体研究内容：a) 代谢路径重构技术与级联生物催化；b) 利用合成生物学理念设计动态调控系统以提高目标产物产量；c) 设计与构建高通量筛选平台定向进化拓展酶的底物专一性和立体选择性；d) 解析天然产物合成路径。


Research Area
KEY WORDS:Synthetic Biology, Metabolic Engineering, Genome Editing, Biocatalysis and Biotransformation, Asymmetric Synthesis, Natural Product and Drug Synthesis.
Our group aims to develop green, sustainable, and cost-effective biosynthesis of high-value pharmaceutical intermediates, fragrance and flavors, fine chemicals and natural products from renewable feedstocks. By employing synthetic biology and metabolic engineering principles, our group focuses on engineering and devising efficient microbial cell factory for chemical productions. More specifically, it aims to a) develop pathway reconstruction technology and cascade biotransformation; b) dynamically regulate the pathway activity via synthetic genetic circuits; c) develop high-throughput screening approaches for directed evolution of substrate specificity and enantioselectivity of key enzymes involved in the downstream cascade biotransformation; and d) elucidate the biosynthetic pathway for natural products.


代表性论文(Selected Publications, # First author * Corresponding author)
1. Cong Fan, Qiwen Mo, Danli Zhang, Jifeng Yuan*. Low-Cost and Noise-Free Yeast Biosensors for On-Site Heavy Metal Pollutant Detection. Submitted.
2. Yueyang Chen, Yufen Chen, Lijun Liu, Yang Zhang, Jifeng Yuan*. A Multi-Enzyme Cascade for 4-Hydroxybenzoate Synthesis From Biobased L-Tyrosine. Submitted.
3. Jifeng Yuan*, Yuling Zhu, Lingrui Liu, Peiling Wu. A Multi-Enzyme Cascade for Efficient Production of L-Phenylglycine from Renewable Feedstock. ACS Sustainable Chemistry and Engineering, 2021, under review.
4. Yang Zhang, Xiaohui Song, Yumeng Lai, Qiwen Mo, Jifeng Yuan*. High-yielding terpene-based biofuel production in Rhodobacter capsulatus. ACS Synthetic Biology, 2021, under review.
5. Yufen Chen^#, Bixia Fu^#, Gezhi Xiao, Liang-Yu Ko, Tzu-Yu Kao, Cong Fan, Jifeng Yuan*. Bioconversion of Lignin-Derived Feedstocks to Muconic Acid by Whole-Cell Biocatalysis. ACS Food Science and Technology, 2021, 1, 3, 382–387
6. Yang Zhang^#, Jifeng Yuan*. CRISPR/Cas12a-mediated genome engineering in the photosynthetic bacterium Rhodobacter capsulatus. Microbial Biotechnology, 2021, doi: 10.1111/1751-7915.13805
7. Qiwen Mo^#, Huiyu Chen^#, Cong Fan^#, Danli Zhang, Lingrui Liu, Bixia Fu, Jifeng Yuan*.Utilization of a styrene-derived pathway for 2-phenylethanol production in budding yeast. Applied Microbiology and Biotechnology, 2021, 105(6), 2333-2340
8. Jifeng Yuan*, Qiwen Mo, Cong Fan. New Set of Yeast Vectors for Shuttle Expression in Escherichia coli. ACS Omega, 2021, 6, 10, 7175–7180
9. Peiling Wu^#, Yufen Chen^#, Mingyu Liu, Gezhi Xiao, Jifeng Yuan*. Engineering an Optogenetic CRISPRi Platform for Improved Chemical Production. ACS Synthetic Biology, 2021, 10, 125-131
10. Yuling Zhu^#, Taiwei Yang^#, Yueyang Chen, Cong Fan, Jifeng Yuan*. One-Pot Synthesis of Aromatic Amines via Whole-Cell Biocatalysis. Chemistryselect, 2020, 5, 14292-14295
11. Yufen Chen^#, Peiling Wu^#, Liang-Yu Ko, Tzu-Yu Kao, Lijun Liu, Yang Zhang, Jifeng Yuan*. High-yielding protocatechuic acid synthesis from L-tyrosine in Escherichia coli. ACS Sustainable Chemistry and Engineering, 2020, 8 (39), 14949-14954
12. Baiyun Zeng^#, Yumeng Lai^#, Lijun Liu, Jie Cheng, Yang Zhang, Jifeng Yuan*. High-yielding hydroxytyrosol synthesis from biobased L-tyrosine via cascade biotransformation. Journal of Agricultural and Food Chemistry, 2020, 68, 29, 7691–7696
13. Zuoxi Mao^#, Lijun Liu^#, Yang Zhang^#, Jifeng Yuan*. Efficient Synthesis of Phenylacetate and 2-Phenylethanol via One-Pot Cascade Biocatalysis. ChemBioChem, 2020, 21, 2676-2679
14. Lijun Liu^#, Yuling Zhu, Yufen Chen, Huiyu Chen, Cong Fan, Qiwen Mo, Jifeng Yuan*. One‐Pot Cascade Biotransformation for Efficient Synthesis of Benzyl Alcohol and Its Analogs. Chemistry - An Asian Journal, 2020, 15,1018-1021
15. Yang Zhang*^#, Jifeng Yuan*, Liejing Guo. Enhanced bio-hydrogen production from cornstalk hydrolysate pretreated by alkaline-enzymolysis with orthogonal design method. International Journal of Hydrogen Energy, 2020, 45(6),3750-3759
16. Jifeng Yuan^#, Benedict Ryan Lukito, Zhi Li*. De novo synthesis of (S)- and (R)-phenylethanediol in yeast via artificial enzyme cascades. ACS Synthetic Biology, 2019, 8(8), 1801-1808
17. Jifeng Yuan*^#, Xue Chen, Pranjul Mishra, Chi-Bun Ching. Metabolically engineered budding yeast for enhanced production of isoamyl alcohol. Applied Microbiology and Biotechnology. 2017, 101(1):465-475
18. Jifeng Yuan*^#, Chi-Bun Ching. Mitochondrial acetyl-CoA utilization pathway for terpenoid productions. Metabolic Engineering. 2016, 38:303-308
19. Jifeng Yuan*^#, Pranjul Mishra, Chi-Bun Ching. Metabolically engineered Saccharomyces cerevisiae for branched-chain ester productions. Journal of Biotechnology. 2017; 239:90-97
20. Wen Xu, Jifeng Yuan*^#, Shuiyun Yang, Chi-Bun Ching, Jiankang Liu. Programming saposin-mediated compensatory metabolic sinks for enhancing ubiquinone productions. ACS Synthetic Biology. 2016, 5:1404-1411
21. Jifeng Yuan*^#, Pranjul Mishra, Chi-Bun Ching. Engineering the leucine biosynthetic pathway for isoamyl alcohol production in budding yeast. Journal of Industrial Microbiology and Biotechnology. 2016, 44(1):107-117
22. Jifeng Yuan*^#, Chi-Bun Ching. Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae. Microbial Cell Factories. 2015, 14, 38
23. Jifeng Yuan*^#, Chi-Bun Ching. Combinatorial assembly of large biochemical pathways into yeast chromosomes for improved production of value-added compounds. ACS Synthetic Biology. 2015, 4:23-31
24. Jifeng Yuan*^#, Chi-Bun Ching. Combinatorial engineering of mevalonate pathway for improved amorpha-4,11-diene production in budding yeast. Biotechnology and Bioengineering. 2014, 111(3):608-17
25. Jifeng Yuan*^#, Hongcai Gao, Jianjun Sui, Hongwei Duan, Wei Ning Chen, Chi-Bun Ching. Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human hepatoma HepG2 cells: An iTRAQ-coupled 2D LC-MS/MS proteome analysis. Toxicological Sciences. 2012, 126(1):149-6
26. Jifeng Yuan*^#, Hongcai Gao, Chi-Bun Ching. Comparative protein profile of human HepG2 cells treated with graphene and single-walled carbon nanotubes: An iTRAQ-coupled 2D LC-MS/MS proteome analysis. Toxicology Letters. 2011, 15; 207(3); 213-21
27. Jifeng Yuan*^#, Hongcai Gao, Jianjun Sui, Wei Ning Chen, Chi-Bun Ching. Cytotoxicity of single-walled carbon nanotubes on human hepatoma HepG2 cells: an iTRAQ-coupled 2D LC-MS/MS proteome analysis. Toxicology In Vitro. 2011, 25(8): 1820-7