杨晓兰
学术学位博士生导师

教授
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检验医学院





学科信息


个人简介
学习（进修）与工作经历
1987.9～1991.7 获西南师范大学化学系理学学士学位；
1991.9～1994.7 获北京师范大学有机化学专业理学硕士学位；
2003.9～2006.7 获重庆医科大学药理学专业博士学位；
1994.7～2003.07，重庆医科大学基础医学院化学教研室任教；
2003.07～2010.12，重庆医科大学药学院药物化学教研室任教，
2011.1～至今，重庆医科大学检验医学院、临床检验诊断学教育部重点实验室。

1996.07，讲师，2006.07，副教授，2011.07，教授，硕士生导师


论文情况
1. Chao Xia#, Jinpeng Lu#, Bangtian Xu, Xiaolei Hu, Yixian Jing, Linyu Yang, Xinpeng Li, Wei Zhou, Gaobo Long, Fei Liao^,*, Xiaolan Yang*^, Design and characterization of a labeling reagent for covalent immobilization of glutathione-S-transferase, Nanosci Nanotechnol Lett, 2019,11(11): 1547-1560. DOI:https://doi.org/10.1166/nnl.2019.3044
2. Yaping Li, Huimin Chong, Xiang Zhang,Xiaolan Yang*, Fei Liao*. High-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag, Anal Biochem,2020; 588:113474. DOI:https://doi.org/10.1166/nnl.2019.3048
3 Yanling Xie, Huimin Chong, Yaping Li, Yiran Feng, Xiaolei Hu, Gaobo Long, Wanjun Xie, Xiaolan Yang*, Fei Liao*. Facile comparison of nonspecific adsorptions of proteins on magnetic submicron particles with alkaline phosphatases as the probes, Nanosci Nanotechnol Lett, 2019,11(11):1574-1581.
4. Xiang Zhang, Shu He, Xiaolei Hu, Jing Wu, Xinpeng Li, Fei Liao*,Xiaolan Yang*(co-corresponding author). Comparison of the Full-Length and 152~528 Truncate of Human Cyclic Nucleotide Phosphodiesterase 4B2 for the Characterization of Inhibitors.Comb Chem High Throughput Screen.2019;22 (1):49-58.
5. Jingjing Rao, Juan Liaob, Youquan Bu, Yitao Wang, Xiaolei Hu, Gaobo Long, MingtongHuang, Luhui Zhong, Xiaolan Yang*, Fei Liao*. Ampholytic ion-exchange materials coated with small zwitterions for high-efficacy purification of ionizable soluble biomacromolecules.,Int J Biol Macromol 2018,120:2234–2241,
6. Mingyue Zou, Xiaolei Hu, Bangtian Xu, Tingting Tong, Yixian Jing, Lei Xi,Wei Zhou, Jingpeng Lu, Xin Wang, Xiaolan Yang*, Fei Liao*. Glutathione S?transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer,Oncology Reports2018,41(2):989-998
7. Cong Luo,Xiaolan Yang, Ming Li, Hua Huang, Quan Kang, Xiaobo Zhang, Hui Hui, Xin Zhang,Chaode Cen, Yujia Luo, Lina Xie, Changxuan Wang, Tongchuan He, Dianming Jiang, Tingyu Li and Hong An. A novel strategy for in vivo angiogenesis and osteogenesis: magnetic micro-movement in a bone scaffold,Artif Cell Nanomed B, 2018, 46(sup2):636-645
8. XiaolanYang, Yiran Feng, Huimin Chong, Deqiang Wang, Xiaolei Hu, Ju Pu, Chang-guo Zhan, Fei Liao *. High-throughput estimation of specific activities of en Hu zyme/mutants in cell lysates through immunoturbidimetric assay of proteins. Anal Biochem. 2017,534:91-98
9. Yiran Feng#, Xiaolan Yang#, Deqiang Wang, Xiaolei Hu, Huimin Chong, Juan Liao, Chang-guo Zhan, Fei Liao*. Polyclonal Antibodies in Microplates to Predict the Maximum Adsorption Activities of Enzyme/Mutants from Cell Lysates.Protein J, 2017,36:212-219
10. Tao Feng#, Xiaolan Yang#, Deqiang Wang, Xiaolei Hu, Juan Liao, Jun Pu, Xinyun Zhao, Chang-Guo Zhan, Fei Liao*. A Practical System for High-Throughput Screening of Mutants of Bacillus fastidiosus Uricase.Appl Biochem Biotechnol. 2017, 181(2):667-681
11. Jun Pu, Xiaolei Hu, Juan Liao, Yuwei Li, Jialin Qin, Yanling Xie, Chang-Guo Zhan, Xiaolan Yang*, Fei Liao*(co-corresponding author). Achievement of linear response for competitive bioaffinity assay of ligand: criteria of optimized interaction system.RSC Adv2016, 6(112): 110858-110865
12. Jing Wu#, Xiaolan Yang#, Deqiang Wang, Xiaolei Hu, Juan Liao, JingJing Rao, Jun Pu, Chang-guo Zhan, Fei Liao*. A numerical approach for kinetic analysis of the nonexponential thermoinactivation process of uricase.Protein J2016, 35(4): 318-329 (DOI: 10.1007/s10930-016-9675-9)
13. Chun Zhang#, Jun Pu#, Xiaolan Yang#, Tao Feng, Fang Liu, Deqiang Wang, Xiaolei Hu, Ang Gao, Hongbo Liu, Chang-Guo Zhan*, Fei Liao*(co-correspondingauthor). Extracorporeal delivery of a therapeutic enzyme.Sci Report2016; 6:30888
14.Yuwei Li#, Xiaolan Yang#, Deqiang Wang, Xiaolei Hu, Mei Yuan, Jun Pu, Chang-Guo Zhan,ZhaoyongYang, Fei Liao*. Striking Effects of Storage Buffers on Apparent Half-Lives of the Activity of Pseudomonas aeruginosa Arylsulfatase. Protein J. 2016. 35(4):283-290.
15. Mei Yuan#, Xiaolan Yang#, Yuwei Li, Hongbo Liu, Jun Pu, Chang-Guo Zhan, Fei Liao*. Facile alkaline lysis of Escherichia coli cells in high-throughput mode for screening enzyme mutants: arylsulfatase as an example. Appl Biochem Biotechnol 2016; 179:545-557 (DOI: 10.1007/s12010-016-2012-0).
16. Liqing Tan#, Tao Jiang#,XiaolanYang#, Wei Li, Lijun Pan,Mingan Yu*.Core–shell biopolymer microspheres for sustained drug release. J Appl Polym Sci 2015,132, 41782 (DOI:10.1002/app.41782)
17. Xiaolan Yang, Xiaolei Hu, Chunyan Chen, Gaobo Long, Jing Wu, Deng Tan, Yuanli Li, Yiran Feng, Jun Pu, Fei Liao*. Comparison of the immobilization of 6His-tagged proteinsonmagnetic-submicron-particle functionalized with Ni²⁺-NTA and bis-sulfone.Nanosci Nanotechn Lett,2015, 7(6):486-494.
18. Hongbo Liu#, Mei Yuan#, Xiaolan Yang#, Xiaolei Hu, Jizheng Dang, Yanling Xie, Jun Pu, Yuanli Li, Chang-Guo Zhan, Fei Liao*. Comparison of candidate pairs of hydrolytic enzymes for spectrophotometric-dual- enzyme-simultaneous-assay.Anal Sci2015, 31(5):421-427.
19. Yuanli Li#, Gaobo Long#, Xiaolan Yang#, Xiaolei Hu, Yiran Feng, Deng Tan, Yanling Xie, Jun Pu, Fei Liao*.Approximated maximum adsorption of His-tagged enzyme/mutants on Ni²⁺-NTA for comparison of specific activities.Int J Biol Macromol2015; 74: 211-217.
20. Ya Huang#, Yuanxiang Chen#, Xiaolan Yang#, Hua Zhao, Xiaolei Hu, Jun Pu, Juan Liao, Gaobo Long, Fei Liao*. Optimization of pH values to formulate the bi-reagent kit for serum uric acid assay.Biotechnol Appl Biochem2015, 62(1):137-144. 6. BangtianXu#, Deng Tan#, Xiaolan Yang#, Xiaolei Hu, Yanling Xie, Jialin Qin, Chunyan Chen, Chenxiong He, Yuanli Li, Jun Pu, Fei Liao*. Fluorometric titration assay of affinity of tight-binding nonfluorescent inhibitor of glutathione s-transferase.J Fluoresc2015, 25(1):1-8
21. Xiaolan Yang, Xiaolei Hu, Bangtian Xu, Xin Wang, Jialin Qin, Chenxiong He, Yanling Xie, YuanliLi, Lin Liu, Fei Liao*. Fluorometric titration approach for calibration of quantity of binding site of purified monoclonal antibody recognizing epitope/hapten nonfluorescent at 340 nm.Anal Chem.2014, 86(12):5667-5672
22. Yuanli Li#, Xiaolan Yang#, Chenxiong He, Xiaolei Hu, Jun Pu, Lin Liu, Gaobo Long, Fei Liao*.Facile quantitative comparison of specific activities of fusion-tagged enzyme/mutants in cell lysates via prediction of their maximum adsorption by anti-tag antibody immobilized in microplate wells.RSC Advance.2014, 4(56): 29925-29932
23. Chun Zhang#, Xiaolan Yang#, Ang Gao, Xiaolei Hu, Jun Pu, Hongbo Liu, Juan Feng, Juan Liao, Yuanli Li, Fei Liao*.Comparison of modification of a bacterial uricase with N-hydroxysuccinimide esters of succinate and carbonate ofmonomethoxylpoly(ethylene glycol).Biotechnol Appl Biochem. 2014, 61(6):683-690
24. Xiaolan Yang, Zhiyin Du, Jun Pu, Hua Zhao, Hong Chen, Yin Liu, Zhirong Li, Zhenli Cheng, Huansi Zhong, Eei Liao*.Classification of difference between inhibition constants of an inhibitor to facilitate identifying the inhibition type.J Enzyme Inhibit Med.Chem.2013,28(1):205-213.
25. Hongbo Liu#, Xiaolan Yang#, Lin Liu, Jizheng Dang, Yanling Xie, Yi Zhang, Jun Pu, Gaobo Long, Yuanli Li, Yonghua Yuan, Juan Liao, Fei Liao*Spectrophotometric-dual-enzyme-simultaneous-assay in one reaction solution (SDESA): chemometrics and experimental models.Anal Chem. 2013, 85 (4):2143-2154.
26. Yi zhang#, Xiaolan Yang#, Lin Liu, Zuexia Huang, Jun Pu, Gaobo Long, Ling Zhang, Dong Liu, Bangtian Xu, Juan Liao, Fei Liao*.Comparison of F?rster-resonance-energy-transfer acceptors for tryptophan and tyrosine residues in native proteins as donors.J Fluorescence2013, 23(1):147-157.
27. Hongbo Liu#, Xiaolan Yang#, Jizheng Dang, Lin Liu, Xiaolei Hu, Jun Pu, Gaobo Long and Fei Liao*.Two glycosidases as label enzymes for concurrent enzyme-linked-immunosorbent-assay of two components via spectrophotometric-dual-enzymesimultaneous-assay in one solution.Anal Methods.2013, 5(10): 5969-5976.
28. Gaobo Long#, Xiaolan Yang#, Yi Zhang, Jun Pu, Lin Liu, Hong-bo Liu, Yuan-li Li, Fei Liao*.Facile one-step coating approach to magnetic submicron particles withpoly(ethylene glycol) coats and abundant accessible carboxyl groups.Int J NanoMed.2013, 8:791-807.
29. Gaobo Long#, Yi Zhang#, Xiaolan Yang#, Jun Pu, Jialin Qin, Lin Liu, Fei Liao*.Facilecharacterizationof the immobilization of streptavidin on magnetic submicron particles with a fluorescent probe of streptavidin.Appl Spectros.2013, 67(6):688-691.
30. Yi Zhang#, Xiaolan Yang#, Xiaolei Hu#, Miaomiao Liu, Chunyan Chen, Yanling Xie, Jun Pu, Jing Wu, Gaobo Long, Fei Liao*.Resonant-Mie- scattering of aggregates of phosphomolybdate and papaverine for measuring activities and screening inhibitors of cyclic nucleotide phosphodiesterase isozymes.Anal Chim Acta2013, 804:215-220.
31. Jizheng Dang#, Hongbo Liu#, Xiaolan Yang#, Yi Zhang, Yanling Xie, Yuanli Li, Jun Pu, Juan Liao,YonghuaYuan, Fei Liao*. Chromogenic substrate from 4-nitro-1-naphthol for hydrolytic enzyme of neutral or slightly acidic optimum pH: 4-nitro-1-naphthyl-β-D-galactopyranoside as an example.Bioorg Med Chem Lett.2013, 23(3):646-649.
32. Xiaolan Yang, Yonghua Yuan, Chang-Guo Zhan*, Fei Liao* Uricases as therapeutic agents to treat refractory gout: current states and future directions.Drug Dev Res.2012, 73(2):66-72
33. Xiaolan Yang, Jun Pu, Hua Zhao, Xiaoyan Li, Juan Liao, Yanling Xie, Sha Zhu, Gaobo Long, YonghuaYuan, Fei Liao.Method to screen aromatic ligands in mixtures for quantitative affinities to target using magnetic separation of bound ligands along with HPLC and UV photometry detection.Michrochim Acta.2012, 176(1):243-249.
34. Ang Gao#, Xiaolan Yang#, Chun Zhang, Gaobo Long, Jun Pu, Yonghua Yuan, Gaobo Long, Yuanli Li, Fei Liao*.Facile spectrophotometric assay of molar equivalents of N-hydroxysuccinimide esters of monomethoxyl poly-(ethylene glycol) derivatives.Chem Cent J.2012, 6(1):142
35. Chun Zhang, Xiaolan Yang, Yong-hua Yuan, Jun Pu, Fei Liao*.Site-specific PEGylation of therapeutic proteins via optimization of both accessible reactive amino acidresiduesand PEG derivatives.Biodrugs.2012 Aug 1;26(4):209-215.
36.Xiaolan Yang,Gaobo Long,Hairong Jiang,Pu Liao,Fei Liao*.Integration of kinetic analysis of reaction curve with a proper classical approach for enzymatic analysis.Scientific World Journal. 2012 May3(Epub) ,2012:969767.
37. Xiaolan Yang, Yanling Xie , Jun Pu, Hua Zhao, Juan Liao, Yiwen Chen, Gaobo Long, Chun Zhang, Yonghua Yuan, Sha Zhu, Huidong Yuan, Fei Liao*. Estimation of affinities of ligands in mixtures via magnetic recovery of target-ligand complexes and chromatographic analyses: chemometrics and an experimental model.BMC Biotechnol.2011, 11:44
38. Xiaolan Yang#,BeizhongLiu#, Yu Sang, Yonghua Yuan, Jun Pu, Yin Liu, Zhirong Li, Juan Feng, Yanling Xie, Renkuan Tang, Huidong Yuan, Fei Liao*. Kinetic analysis of lactate-dehydrogenase-coupled reaction process and measurement of alanine transaminase by an integration strategy.Anal Sci. 2010, 26(11):1193-1198.
39. Yanling Xie#, Xiaolan Yang#, Jun Pu, Yunsheng Zhao, Ying Zhang, Guoming Xie, Jun Zheng, Huidong Yuan, Fei Liao*.Homogeneous competitive assay of ligand affinities based on quenching fluorescence of tyrosine/tryptophanresiduesin a protein via FOrster-resonance-energy -transfer.Spectrochim Acta A Mol Biomol Spectrosc.2010, 77(4):869-876.
40. Chun Zhang#, Xiaolan Yang#, Juan Feng, Yonghua Yuan, Xiang Li, Youquan Bu, Yanling Xie, Huidong Yuan, Fei Liao*. Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus.Biosci Biotechnol Biochem2010, 74(6): 1298-1301.





知识产权
4.廖飞，杨晓兰，胡小蕾，冯一然，种慧敏，李雨薇。一种均相定量比较未纯化酶及其突变体的方法。国家发明专利，ZL5.X。授权日期2018-08-10。
5.廖飞，杨晓兰，张纯，刘红博，高昂。在竞争性抑制剂保护下用针对氨基的活化聚乙二醇修饰尿酸酶的方法。国家发明专利,ZL4.6。授权日期2018-07-06。
6.廖飞，杨晓兰，胡小蕾，许榜田，王昕，陈春艳，秦家林，谭登，黄亚。双-对烷氧基苯丙烯酮类谷胱甘肽-S-转移酶潜抑制剂。国家发明专利，ZL4.4。授权日期2018-03-27。
7.Liao Fei, Yang Xiaolan, Liu Hongbo, Dang jizheng, Long Gaobo, Li Yuanli, Liu Lin. Method for simultaneously measuring activity of various enzymes by using multiple-wavelength absorption single channel. US**B2, Date of patent, April 4, 2017.
8.廖飞，杨晓兰，胡小蕾，秦家林，何琛雄，李元丽，刘霖。一种荧光滴定测定单抗活性位点浓度的方法。国家发明专利，ZL 5.6。授权日期2018-06-08。
9.廖飞，杨晓兰，胡小蕾，李元丽，陈春艳，刘霖。一种快速比较未纯化融合酶及其突变体比活性的方法。中国发明专利，受理号 7.6. 授权日期2016-5-30
10.廖飞，杨晓兰，党纪政，刘红博，张奕，李元丽。一种用4-硝基-1-萘酚或其衍生物为显色团制备?-半乳 糖苷酶显色底物的方法及其应用。中国发明专利，ZL 1.0。授权日期2016-01-06。
11.廖飞，杨晓兰，刘红博，党济政，龙高波，李元丽，刘霖。用多波长吸收单通道同步测量多种酶活性的方法。中国发明专利，ZL6.8；授权日期2014-10-29；IPC 分类号G01N21/31(2006.01)I；PCT专利受理号PCT/CN2012/081869，进入美国。
12.廖飞，杨晓兰，龙高波，蒲军，刘红博，李元丽。用两端带羧基的直链亲水聚合物包被纳米磁芯制备微纳米磁性材料的方法及其应用。中国发明专利，ZL 9.5. 授权日期2014-09-24. IPC 分类号C08F290/06(2006.01)I
13.廖飞，杨晓兰，龙高波，刘红博，廖娟。联用酶反应动力学过程分析法和终点平衡法测定酶底物量的方法。中国发明专利，ZL 6.5. 授权日期2014-09-24. IPC 分类号G01N21/31(2006.01)I
14.廖飞，杨晓兰，袁拥华，赵运胜，冯娟，张纯，谢燕玲。作用于血液中低分子物质的大分子药物的体外给药装置及其应用。中国发明专利，ZL 0.X；授权日期2014-08-27. IPC 分类号 A61M1/36(2006.01)I
15.廖飞，杨晓兰，谢燕玲，邓萍，赵运胜，朱莎，甘志勇，廖娟，刘冬，于明安。 联用磁分离与色谱分析测定混合物中配体亲和力的方法。中国发明专利，ZL 8.2，授权日期2012-09-05. IPC 分类号G01N30/00(2006.01)I



科研项目

课题来源 起讫年月 经费
（万元）
指数富集快速发现天然产物混合物中抗结核活性先导配体cstc2019jcyj-msxmX0166 重庆市科学技术局 2019.12.27-2.22.06.30
10
混合物中低丰度高亲和力配体的指数富集技术及应用”，编号** 国家自然科学基金面上项目 2015.09.01-2019.12.30 60
科技部863IVD领域重大专项(预研)项目“新型免疫检测技术的研究”编号2011AA02A108;子课题6:“新型试剂材料制备” 科技部863重大专项(预研)项目子课题 2012.05.08 11
融合表达标签GST高亲和力配体的设计筛选与应用(CSTC2012JJA0057) 部省级面上项目 2012.08.28 2.5



成果奖励
1.罗聪, 安洪, 蒋电明, 杨晓兰, 张健, 邹海波, 朱照静. 重庆市卫生局医学科技二等奖: 震荡磁场促超顺磁性明胶微球质粒释放的实验研究. 编号2010-26-7
2.余瑜, 陈志琼, 杨晓兰, 李龙江, 汤为学, 成禹杉, 刘嫱, 罗宗伟, 林琳, 黄维, 李伟.重庆市科学技术发明三等奖: 非细胞毒性抗肿瘤化合物德氮吡格注射液的开发研究. FM,2007.3.



著作教材
1. Xiaolan Yang, Gaobo Long, Hua Zhao and Fei Liao*. Kinetic Analyses of Enzyme Reaction Curves with New Integrated Rate Equations and Applications, in Chemometrics in Practical Applications (2012). pp.157-184. Kurt Varmuza (Ed.), ISBN: 978-953-51-0438-4, InTech







研究方向
生物标记物检测新方法和新材料，药物先导化合物发现的高通量筛选新方法