Abstract:Biotreatment of real domestic sewage by algae and bacteria in a sequencing batch mode using a photobioreactor was investigated. The algae from the wall of the secondary sedimentation tank, and the activated sludge from the aeration tank of the same municipal wastewater treatment plant, were inoculated into the photobioreactor as the algae-bacteria inoculum. The effect of sludge and algae inoculation ratios on sewage treatment was discussed and the composition of microbial community during the stable operation was analyzed. The intermittent experiments showed that, when the mass ratio of sludge to algae was 1∶0.75, the highest removal efficiencies of COD, TN and TP occurred. In the photobioreactor inoculated with sludge-algae mass ratio of 1∶0.75, under the conditions of the initial TSS of 1.12 g·L?1 and the hydraulic retention time of 2 days, as well as sunlight irradiation and stirring, the removal efficiencies of ${\rm{NH}}_4^ + $-N and ${\rm{PO}}_4^{3 - }$ could reach 99.7% and 70%, respectively. Using high-throughput sequencing technologies to analyze the bacterial and fungal community composition in the photobioreactor with a SRT of 15 days after 42-day running, it was found that the predominant bacterial genus were Exiguobacterium in the Phylum Firmicutes, Cyanobium in the Phylum Cyanobacteria, and Rhodobacter in the Class Alphaproteobacteria, their relative abundances were 23.32%, 15.23% and 5.77%, respectively. At the same time, Nitrospira for nitrite oxidization, Acinetobacter for phosphorus removal and Paracoccus for aerobic denitrification, were also observed in the reactor, with the relative abundances of 1.19%, 0.58% and 0.35%, respectively. Key words:sludge-algae photobioreactor/ mass inoculation ratio of sludge to algae/ domestic sewage/ bacterial community.
图1实验装置示意图 Figure1.Schematic diagram of experimental device
图2不同接种比的反应器COD、TN 和 TP出水质量浓度的变化 Figure2.Effluent concentrations of COD, TN and TP in the reactors with different inoculation ratios of algae to activated sludge
NGUYEN T T D, NGUYEN T T, BINH Q A, et al. Co-culture of microalgae-activated sludge for wastewater treatment and biomass production: Exploring their role under different inoculation ratios[J]. Bioresource Technology, 2020, 314: 123754. doi: 10.1016/j.biortech.2020.123754
[2]
KATAM K, BHATTACHARY A D. Simultaneous treatment of domestic wastewater and bio-lipid synthesis using immobilized and suspended cultures of microalgae and activated sludge[J]. Journal of Industrial and Engineering Chemistry, 2019, 69: 295-303. doi: 10.1016/j.jiec.2018.09.031
[3]
LEONG W H, LIM J W, LAM M K, et al. Co-cultivation of activated sludge and microalgae for the simultaneous enhancements of nitrogen-rich wastewater bioremediation and lipid production[J]. Journal of the Taiwan Institute of Chemical Engineers, 2018, 87: 216-224. doi: 10.1016/j.jtice.2018.03.038
[4]
ZHU S, QIN L, FENG P, et al. Treatment of low C/N ratio wastewater and biomass production using co-culture of Chlorella vulgaris and activated sludge in a batch photobioreactor[J]. Bioresource Technology, 2019, 274: 313-320. doi: 10.1016/j.biortech.2018.10.034
[5]
JI X, JIANG M, ZHANG J, et al. The interactions of algae-bacteria symbiotic system and its effects on nutrients removal from synthetic wastewater[J]. Bioresource Technology, 2018, 247: 44-50. doi: 10.1016/j.biortech.2017.09.074
[6]
HUO S, KONG M, ZHU F, et al. Co-culture of Chlorella and wastewater-born bacteria in vinegar production wastewater: Enhancement of nutrients removal and influence of algal biomass generation[J]. Algal Research, 2020, 45: 101744. doi: 10.1016/j.algal.2019.101744
[7]
GUTZEIT G, LORCH D, WEBER A, et al. Bioflocculent algal-bacterial biomass improves low-cost wastewater treatment[J]. Water Science and Technology, 2005, 52: 9-18.
[8]
SU Y, MENNERICH A, URBAN B. Synergistic cooperation between wastewater-born algae and activated sludge for wastewater treatment: Influence of algae and sludge inoculation ratios[J]. Bioresource Technology, 2012, 105: 67-73. doi: 10.1016/j.biortech.2011.11.113
[9]
MUJTABA G, LEE K. Treatment of real wastewater using co-culture of immobilized Chlorella vulgaris and suspended activated sludge[J]. Water Research, 2017, 120: 174-184. doi: 10.1016/j.watres.2017.04.078
SERENA P, PAOLA F, FRANCESCO B, et al. How inoculation affects the development and the performances of microalgal-bacterial consortia treating real municipal wastewater[J]. Journal of Environmental Management, 2020, 263: 110427. doi: 10.1016/j.jenvman.2020.110427
[15]
GARC A D, POSADA S E, BLANCO S, et al. Evaluation of the dynamics of microalgae population structure and process performance during piggery wastewater treatment in algal-bacterial photobioreactors[J]. Bioresource Technology, 2018, 248: 120-126. doi: 10.1016/j.biortech.2017.06.079
[16]
DONNA L S, CLIVE H W, MATTHEW H T, et al. Enhancing microalgal photosynthesis and productivity in wastewater treatment high rate algal ponds for biofuel production[J]. Bioresource Technology, 2015, 184: 222-229. doi: 10.1016/j.biortech.2014.10.074
[17]
BARROS A I, GONALVES A L, SIMOES M, et al. Harvesting techniques applied to microalgae: A review[J]. Renewable & Sustainable Energy Reviews, 2015, 41: 1489-1500.
[18]
CHEVALIER P, NOüE J. Efficiency of immobilized hyperconcentrated algae for ammonium and phosphate removal from wastewaters[J]. Biotechnology Letters, 1985, 7(6): 395-400. doi: 10.1007/BF01166210
[19]
胡子全. 好氧聚磷菌的分离、鉴定及其聚磷特性的研究[D]. 合肥: 安徽农业大学, 2008.
[20]
SUTHERLAND D L, HOWARD-WILLIAMS C, TURNBULL M H, et al. The effects of CO2 addition along a pH gradient on wastewater microalgal photo-physiology, biomass production and nutrient removal[J]. Water Research, 2015, 70: 9-26. doi: 10.1016/j.watres.2014.10.064
RUPERT C, DONNA S, HELENA C. Hectare-scale demonstration of high rate algal ponds for enhanced wastewater treatment and biofuel production[J]. Journal of Applied Phycology, 2012, 24(3): 329-337. doi: 10.1007/s10811-012-9810-8
[23]
SUTHERLAND D L, HOWARD-WILLIAMS C, TURNBULL M H, et al. Seasonal variation in light utilization, biomass production and nutrient removal by wastewater microalgae in a full-scale high-rate algal pond[J]. Journal of Apply Phycology, 2013, 26(3): 1317-1329.
DUCAT D C, AVELAR-RIVAS J A, WAY J C, et al. Rerouting carbon flux to enhance photosynthetic productivity[J]. Applied and Environmental Microbiology, 2012, 78(8): 2660-2668. doi: 10.1128/AEM.07901-11
[26]
LI T T, LI C T, BUTLER K, et al. Mimicking lichens: Incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal and lipid production in a stable mutualistic co-culture production platform[J]. Biotechnology for Biofuels, 2017, 10: 55. doi: 10.1186/s13068-017-0736-x
[27]
BERNSTEIN H C, MC CLURE R S, THIEL V, et al. Indirect interspecies regulation: transcriptional and physiological responses of a cyanobacterium to heterotrophic partnership[J]. mSystems, 2017, 2(2): e00181-16.
[28]
SERGEY A K, GUIFR T, MOREIRA D, et al. Molecular phylogeny of paraphelidium letcheri sp. nov. (Aphelida, Opisthosporidia)[J]. Eukaryotic Microbiology, 2017, 64(5): 573-578. doi: 10.1111/jeu.12389
[29]
BOENIGK J. The Choanoflagellates: Evolution, biology and ecology[J]. Marine Biology Research, 2015, 11(10): 1118-1119. doi: 10.1080/17451000.2015.1080846
Fujian Key University Laboratory of Estuarine Ecological Security and Environmental Health, School of Environmental Science and Engineering of Xiamen University TanKah Kee College, Zhangzhou 363105, China Received Date: 2021-02-09 Accepted Date: 2021-04-23 Available Online: 2021-07-23 Keywords:sludge-algae photobioreactor/ mass inoculation ratio of sludge to algae/ domestic sewage/ bacterial community Abstract:Biotreatment of real domestic sewage by algae and bacteria in a sequencing batch mode using a photobioreactor was investigated. The algae from the wall of the secondary sedimentation tank, and the activated sludge from the aeration tank of the same municipal wastewater treatment plant, were inoculated into the photobioreactor as the algae-bacteria inoculum. The effect of sludge and algae inoculation ratios on sewage treatment was discussed and the composition of microbial community during the stable operation was analyzed. The intermittent experiments showed that, when the mass ratio of sludge to algae was 1∶0.75, the highest removal efficiencies of COD, TN and TP occurred. In the photobioreactor inoculated with sludge-algae mass ratio of 1∶0.75, under the conditions of the initial TSS of 1.12 g·L?1 and the hydraulic retention time of 2 days, as well as sunlight irradiation and stirring, the removal efficiencies of ${\rm{NH}}_4^ + $-N and ${\rm{PO}}_4^{3 - }$ could reach 99.7% and 70%, respectively. Using high-throughput sequencing technologies to analyze the bacterial and fungal community composition in the photobioreactor with a SRT of 15 days after 42-day running, it was found that the predominant bacterial genus were Exiguobacterium in the Phylum Firmicutes, Cyanobium in the Phylum Cyanobacteria, and Rhodobacter in the Class Alphaproteobacteria, their relative abundances were 23.32%, 15.23% and 5.77%, respectively. At the same time, Nitrospira for nitrite oxidization, Acinetobacter for phosphorus removal and Paracoccus for aerobic denitrification, were also observed in the reactor, with the relative abundances of 1.19%, 0.58% and 0.35%, respectively.
下载: 
点击查看大图
