Influence of particles on the detection of Giardia and Cryptosporidium by membrane concentration/density gradient separation and immunofluorescence method
ZHANG Yanfen1,, LI Hongyan1,,, LIU Mingyang2, ZHENG Bei1 1.Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China 2.School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China
Abstract:Due to its low cost and stable performance, the membrane concentration/density gradient separation and immunofluorescence method has been listed in the Standard of Water Quality Examination Methods for Urban Water Supply (CJ/T 141-2018) for the detection of Giardia and Cryptosporidium in urban supplied water and source water. In view of the fact that particles in water will affect the recovery and stability of this method, based on the previous research and in combination of particle size, the influence of turbidity and particle size on the detection of Giardia and Cryptosporidium in water was further explored. Results showed that particle size contributing to the turbidity of water only affected the recovery of Cryptosporidium, while had no significant effect on the test of Giardia, of which the highest recovery of Cryptosporidium occurred in water mainly containing particles with 5 μm size. Turbidity is the main factor affecting the recovery of this method. The membrane concentration method was preferred to monitor Giardia and Cryptosporidium in water samples with turbidity below 20 NTU, with the best recovery at 3~5 NTU. The sedimentation concentration method was suitable for water samples with turbidity higher than 20 NTU, with the best recovery for Giardia and Cryptosporidium at 30~50 NTU. Key words:Giardia/ Cryptosporidium/ turbidity/ particle size/ density gradient separation and immunofluorescence method.
图1颗粒物大小对隐孢子虫回收率的影响 Figure1.Effect of particle size on Cryptosporidium recovery
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Influence of particles on the detection of Giardia and Cryptosporidium by membrane concentration/density gradient separation and immunofluorescence method
1.Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China 2.School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China Received Date: 2020-09-18 Accepted Date: 2020-12-21 Available Online: 2021-05-08 Keywords:Giardia/ Cryptosporidium/ turbidity/ particle size/ density gradient separation and immunofluorescence method Abstract:Due to its low cost and stable performance, the membrane concentration/density gradient separation and immunofluorescence method has been listed in the Standard of Water Quality Examination Methods for Urban Water Supply (CJ/T 141-2018) for the detection of Giardia and Cryptosporidium in urban supplied water and source water. In view of the fact that particles in water will affect the recovery and stability of this method, based on the previous research and in combination of particle size, the influence of turbidity and particle size on the detection of Giardia and Cryptosporidium in water was further explored. Results showed that particle size contributing to the turbidity of water only affected the recovery of Cryptosporidium, while had no significant effect on the test of Giardia, of which the highest recovery of Cryptosporidium occurred in water mainly containing particles with 5 μm size. Turbidity is the main factor affecting the recovery of this method. The membrane concentration method was preferred to monitor Giardia and Cryptosporidium in water samples with turbidity below 20 NTU, with the best recovery at 3~5 NTU. The sedimentation concentration method was suitable for water samples with turbidity higher than 20 NTU, with the best recovery for Giardia and Cryptosporidium at 30~50 NTU.
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