Effect of redox mediator on sewage biological denitrifying phosphorus removal at low temperature and the change of phosphorus forms
YUAN Hongying1,2,3,,, WANG Hongbin1,2, LI Yuanling1,2, WANG Xue1,2, ZHAO Xin1,2 1.School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China 2.Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin 300384, China 3.Joint Research Centre for Protective Infrastructure Technology and Environmental Green Bioprocess, Tianjin 300384, China
Abstract:The sewage biological denitrifying phosphorus removal technology can overcome the deficiency of carbon source in the traditional biological nitrogen and phosphorus removal process. It use nitrate as an electron acceptor instead of oxygen. Nitrogen, phosphorus and organic matter removal occurs at the same time to achieve “one-carbon dual-use”. But denitrifying phosphorus removal efficiency will decrease at low temperature. In this study, the change of denitrifying phosphorus removal efficiency was investigated by adding the redox mediator 1, 2-naphthoquinone-4-sulfonic (NQS) in sequential batch reactors (SBRs) (90 min anaerobic, 330 min anoxic, 1# (the control), 2#(adding the redox mediator)) at low temperature(10±1) ℃. The results showed that the addition of NQS increased the sewage biological denitrifying phosphorus removal at low temperature compared with the control experiment. The total nitrogen removal rate increased from 37.67% to 51.47%, which increased by 1.37 times, and the total phosphorus removal rate increased from 53.45% to 96.50%, which increased by 1.81 times. In addition, the NQS promoted the transformation of phosphates to organic phosphorus (Org-P) in sewage, the main forms of phosphorus in the sludge were dissolved reactive phosphate (SRP) and Org-P, with the maximum contents of 35.78 mg?L?1 and 51.09 mg?L?1, respectively. In the control reactor, the main forms of phosphorus in the sludge were SRP and Fe-P, with the maximum contents of 45.61 mg?L?1 and 40.67 mg?L?1, respectively. This study provides theoretical support for improving the effect of biological denitrification phosphorus removal in wastewater at low temperature. Key words:redox mediator/ denitrifying phosphorus removal/ SRP/ Org-P.
图1SBR 装置示意图 Figure1.Schematic diagram of SBR installation
RAHIMNEJAD M, NAJAFPOUR G D, GHOREYSHI A A, et al. Thionine increases electricity generation from microbial fuel cell using Saccharomyces cerevisiae and exoelectrogenic mixed culture[J]. Journal of Microbiology, 2012, 50(4): 575-580. doi: 10.1007/s12275-012-2135-0
[4]
CERVANTES-CARRILLO F J. Quinones as electron acceptors and redox mediators for the anaerobic biotransformation of priority pollutants[D]. Wageningen: Wageningen University, 2002.
LI L, WANG J, ZHOU J, et al. Enhancement of nitroaromatic compounds anaerobic biotransformation using a novel immobilized redox mediator prepared by electropolymerization[J]. Bioresource Technology, 2008, 99(15): 6908-6916. doi: 10.1016/j.biortech.2008.01.037
[7]
SUN J, LI W, LI Y, et al. Redox mediator enhanced simultaneous decolorization of azo dye and bioelectricity generation in air-cathode microbial fuel cell[J]. Bioresource Technology, 2013, 142: 407-414. doi: 10.1016/j.biortech.2013.05.039
[8]
HUANG W, CHEN J, HU Y, et al. Enhanced simultaneous decolorization of azo dye and electricity generation in microbial fuel cell (MFC) with redox mediator modified anode[J]. International Journal of Hydrogen Energy, 2017, 42(4): 2349-2359. doi: 10.1016/j.ijhydene.2016.09.216
FYTIANOS K, KOTZAKIOTI A. Sequential fractionation of phosphorus in lake sediments of Northern Greece[J]. Environmental Monitoring and Assessment, 2005, 100(1/2/3): 191-200.
[12]
KAISERLI A, VOUTSA D, SAMARA C. Phosphorus fractionation in lake sediments: Lakes Volvi and Koronia, N. Greece[J]. Chemosphere, 2002, 46(8): 1147-1155. doi: 10.1016/S0045-6535(01)00242-9
[13]
YIN X, QIAO S, ZHOU J, et al. Effects of redox mediators on nitrogen removal performance by denitrifying biomass and the activity of nar and nir[J]. Chemical Engineering Journal, 2014, 257: 90-97. doi: 10.1016/j.cej.2014.07.029
[14]
ZHOU S, ZHANG X, FENG L. Effect of different types of electron acceptors on the anoxic phosphorus uptake activity of denitrifying phosphorus removing bacteria[J]. Bioresource Technology, 2010, 101(6): 1603-1610. doi: 10.1016/j.biortech.2009.09.032
[15]
XI Z, GUO J, LIAN J, et al. Study the catalyzing mechanism of dissolved redox mediators on bio-denitrification by metabolic inhibitors[J]. Bioresource Technology, 2013, 140: 22-27. doi: 10.1016/j.biortech.2013.04.065
[16]
UCHIMIYA M, STONE A T. Reversible redox chemistry of quinones: Impact on biogeochemical cycles[J]. Chemosphere, 2009, 77(4): 451-458. doi: 10.1016/j.chemosphere.2009.07.025
[17]
KLüPFEL L, PIEPENBROCK A, KAPPLER A, et al. Humic substances as fully regenerable electron acceptors in recurrently anoxic environments[J]. Nature Geoscience, 2014, 7(3): 195-200. doi: 10.1038/ngeo2084
[18]
DOS SANTOS A B, BISSCHOPS I A E, CERVANTES F J, et al. Effect of different redox mediators during thermophilic azo dye reduction by anaerobic granular sludge and comparative study between mesophilic (30 ℃) and thermophilic (55 ℃) treatments for decolourisation of textile wastewaters[J]. Chemosphere, 2004, 55(9): 1149-1157. doi: 10.1016/j.chemosphere.2004.01.031
HANSEN K A S. Removal of Phosphate by Poly-P Accumulating Organisms in Activated Sludge and Their Fate in Digesters[M]. Aalborg: Aalborg Universitetsforlag, 2019.
1.School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China 2.Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin 300384, China 3.Joint Research Centre for Protective Infrastructure Technology and Environmental Green Bioprocess, Tianjin 300384, China Received Date: 2020-05-07 Accepted Date: 2020-07-09 Available Online: 2021-02-22 Keywords:redox mediator/ denitrifying phosphorus removal/ SRP/ Org-P Abstract:The sewage biological denitrifying phosphorus removal technology can overcome the deficiency of carbon source in the traditional biological nitrogen and phosphorus removal process. It use nitrate as an electron acceptor instead of oxygen. Nitrogen, phosphorus and organic matter removal occurs at the same time to achieve “one-carbon dual-use”. But denitrifying phosphorus removal efficiency will decrease at low temperature. In this study, the change of denitrifying phosphorus removal efficiency was investigated by adding the redox mediator 1, 2-naphthoquinone-4-sulfonic (NQS) in sequential batch reactors (SBRs) (90 min anaerobic, 330 min anoxic, 1# (the control), 2#(adding the redox mediator)) at low temperature(10±1) ℃. The results showed that the addition of NQS increased the sewage biological denitrifying phosphorus removal at low temperature compared with the control experiment. The total nitrogen removal rate increased from 37.67% to 51.47%, which increased by 1.37 times, and the total phosphorus removal rate increased from 53.45% to 96.50%, which increased by 1.81 times. In addition, the NQS promoted the transformation of phosphates to organic phosphorus (Org-P) in sewage, the main forms of phosphorus in the sludge were dissolved reactive phosphate (SRP) and Org-P, with the maximum contents of 35.78 mg?L?1 and 51.09 mg?L?1, respectively. In the control reactor, the main forms of phosphorus in the sludge were SRP and Fe-P, with the maximum contents of 45.61 mg?L?1 and 40.67 mg?L?1, respectively. This study provides theoretical support for improving the effect of biological denitrification phosphorus removal in wastewater at low temperature.
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