Denitrification efficiency and microbiological analysis of sludge with addition of the pretreated landfill leachate
LIU Huaguang1,, RONG Chao2, ZHANG Jinsong1,2,3,4, ZHOU Xingyu2,3,4,, 1.School of Civil Engineering, Guangzhou University, Guangzhou 510006, China 2.Shenzhen Water (Group) Co. Ltd., Shenzhen 518031, China 3.Shenzhen Municipal Sewage Treatment and Recycling Engineering Laboratory, Shenzhen 518001, China 4.School of Environment, Harbin Institute of Technology, Harbin 150090, China
Abstract:In order to explore the feasibility of pretreated landfill leachate (PLL) as an additional carbon source for the removal of high concentration nitrate nitrogen, SBR system R0 and R1 were established(R0: anhydrous sodium acetate carbon source; R1: PLL + anhydrous sodium acetate carbon source). Efficient denitrifying activated sludge was obtained through cultivation with the simulated high concentration nitrate nitrogen wastewater and PLL addition from anaerobic biofilter. Then its denitrification efficiency was investigated, and the molecular biological was also analyzed. The results showed that nitrate nitrogen could be almost completely removed within 2.5 hours in R1 system at PLL addition volume fraction of 10%, and the denitrification rate reached 58.05 mg·(g·h)?1, which was 1.79 times of R0. 16S rDNA sequencing showed that the microbial population structure of R0 and R1 reactors were comparatively similar. The dominant denitrifying bacteria in the first three abundances were pseudomonas, thauera and pannonibacter, but their relative abundances were different; The results of qPCR showed that the relative expression of denitrification genes narG, nirK, nirS and norB in experimental group R1 was significantly higher than that in control group R0. PLL as an additional carbon source could promote the sludge denitrification activity. Key words:pretreated landfill leachate/ high concentration nitrate/ denitrification/ microbial community structure.
图1SBR反应装置示意图 Figure1.Schematic diagram of the SBR reaction unit
图2R1系统在添加PLL前后${{\bf{NO}}_3^{-}} $-N浓度的变化和对${{\bf{NO}}_3^{-}} $-N的去除率 Figure2.Variation of ${{\rm{NO}}_3^{-}} $-N concentration and removal efficiency of ${{\rm{NO}}_3^{-}}$-N in R1 system before and after adding pretreated landfill leachate
图4不同C/N在不同前处理垃圾渗滤液添加比例条件下VDN和COD去除率 Figure4.Removal rates of VDN and COD under the conditions of different C/N and pretreated landfill leachate addition ratios
图5R0和R1系统运行第65天时不同分类水平上的微生物群落结构 Figure5.Microbial community structure at different taxonomic levels in R0 and R1 systems on the 65th day of stable operation
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1.School of Civil Engineering, Guangzhou University, Guangzhou 510006, China 2.Shenzhen Water (Group) Co. Ltd., Shenzhen 518031, China 3.Shenzhen Municipal Sewage Treatment and Recycling Engineering Laboratory, Shenzhen 518001, China 4.School of Environment, Harbin Institute of Technology, Harbin 150090, China Received Date: 2019-02-21 Accepted Date: 2019-04-26 Available Online: 2020-11-11 Keywords:pretreated landfill leachate/ high concentration nitrate/ denitrification/ microbial community structure Abstract:In order to explore the feasibility of pretreated landfill leachate (PLL) as an additional carbon source for the removal of high concentration nitrate nitrogen, SBR system R0 and R1 were established(R0: anhydrous sodium acetate carbon source; R1: PLL + anhydrous sodium acetate carbon source). Efficient denitrifying activated sludge was obtained through cultivation with the simulated high concentration nitrate nitrogen wastewater and PLL addition from anaerobic biofilter. Then its denitrification efficiency was investigated, and the molecular biological was also analyzed. The results showed that nitrate nitrogen could be almost completely removed within 2.5 hours in R1 system at PLL addition volume fraction of 10%, and the denitrification rate reached 58.05 mg·(g·h)?1, which was 1.79 times of R0. 16S rDNA sequencing showed that the microbial population structure of R0 and R1 reactors were comparatively similar. The dominant denitrifying bacteria in the first three abundances were pseudomonas, thauera and pannonibacter, but their relative abundances were different; The results of qPCR showed that the relative expression of denitrification genes narG, nirK, nirS and norB in experimental group R1 was significantly higher than that in control group R0. PLL as an additional carbon source could promote the sludge denitrification activity.
模拟废水水质。实验以模拟高浓度硝态氮废水为考察对象，驯化阶段用无水乙酸钠为COD唯一来源，启动成功后添加PLL作为混合碳源；利用KNO3作为唯一氮源；以KH2PO4配制磷酸盐质量浓度；以MgSO4和CaCl2满足反硝化菌对Mg2+和Ca2+的需求；微量元素添加量为1 mL·L?1，1 L微量元素溶液中含有10.00 g EDTA、1.50 g FeCl3·6H2O、0.03 g CuSO4·5H2O、0.12 g ZnSO4·7H2O、0.15 g CoCl2·6H2O、0.12 g MnCl2·4H2O、0.06 g Na2MoO4·2H2O、0.18 g KI、0.15 g H3BO3；实验全过程各反应器内DO ≤ 0.4 mg·L?1，保持缺氧状态；进水pH控制在7.5 ± 0.1；水温为室温，即21.5~25.6 ℃。 前处理垃圾渗滤液水质及来源。深圳市下坪垃圾填埋场采用氨吹脱+厌氧生物滤池+A/A/O+MBR+NF的新型组合工艺处理垃圾渗滤液，本研究所用前处理垃圾渗滤液取自该垃圾填埋场厌氧生物滤池出水，此系统前2步流程已经有效降解垃圾渗滤液原液中的氨氮、SS等主要污染物，前处理垃圾渗滤液中的COD主要来源于VFAs和腐殖酸。具体水质成分如下：COD (1 989±50) mg·L?1，${\rm{NH}}_4^{+} $-N (8.5±3.5) mg·L?1，DO (0.2±0.1) mg·L?1，Ca2+ (335±14) mg·L?1，Mg2+ (13.2±3.8) mg·L?1，As (0.253±0.096) mg·L?1，Cd (0.04±0.01) mg·L?1。
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