Effects of temperature and initial pH on the hydrogen production by dark fermentation of agricultural solid waste
JIA Xuan1,, LI Yingxin2, WU Yanan1, WANG Yong3, HAO Yan3, LI Mingxiao3,, 1.Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light industry, Beijing Technology and Business University, Beijing 100048, China 2.Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China 3.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
Abstract:In this study, five kinds of typical agricultural solid wastes including pig feces, chicken feces, corn stover, food waste and kitchen waste were taken as substrates, and the effects of temperature and initial pH on their hydrogen production potential by dark fermentation were determined by using the modified Gompertz model, and the main pathways of hydrogen production and metabolism were analyzed. The results showed that temperature and initial pH had significant effects on the hydrogen production by dark fermentation of agricultural solid wastes. The cumulative gas production and hydrogen content in high temperature group were significantly higher than those in medium temperature group. At high temperature of 55 ℃ and pH 6.0, the best dark fermentation effect of food wastes was achieved, and the highest cumulative gas production and hydrogen content were obtained with respective value of 1 100 mL and 73.58%, and the maximum hydrogen production rate and hydrogen production potential were 37.11 mL·h?1 and 660.30 mL, which were followed by kitchen waste, while chicken feces had the worst hydrogen production potential. The concentration of ammonia nitrogen was the highest at the end of hydrogen production by dark fermentation of chicken feces. Excessive concentration of ammonia nitrogen might inhibit the hydrogen production process. VFA analysis showed that the concentration of butyric acid was the highest under different substrates and conditions, and it also contained a small amount of ethanol, acetic acid, propionic acid. The hydrogen production pathway was a mixed fermentation based on butyric acid fermentation. Through optimizing and controlling the non-biological control factors such as temperature and initial pH, the potential of hydrogen production by dark fermentation of agricultural solid wastes and the utilization efficiency of biomass were significantly improved, which provided a theoretical basis for the research and development of biological hydrogen production technology and engineering application. Key words:agricultural solid waste/ dark fermentation/ pH/ temperature/ metabolites/ dynamic analysis.
图1不同温度和初始pH条件下积累产气量和氢气百分含量 Figure1.Influence of cumulative gas production and hydrogen content at different temperatures and initial pHs
图2不同温度和初始pH条件下Gompertz模拟累积产氢的变化趋势 Figure2.Change trend of accumulated hydrogen production simulated by Gompertz at different temperatures and initial pHs
图4不同温度和初始pH条件下暗发酵过程氨氮浓度的变化趋势 Figure4.Variation trend of ammonia nitrogen concentration during dark fermentation at different temperatures and initial pHs
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1.Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light industry, Beijing Technology and Business University, Beijing 100048, China 2.Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China 3.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China Received Date: 2018-10-09 Accepted Date: 2019-03-27 Available Online: 2019-09-17 Keywords:agricultural solid waste/ dark fermentation/ pH/ temperature/ metabolites/ dynamic analysis Abstract:In this study, five kinds of typical agricultural solid wastes including pig feces, chicken feces, corn stover, food waste and kitchen waste were taken as substrates, and the effects of temperature and initial pH on their hydrogen production potential by dark fermentation were determined by using the modified Gompertz model, and the main pathways of hydrogen production and metabolism were analyzed. The results showed that temperature and initial pH had significant effects on the hydrogen production by dark fermentation of agricultural solid wastes. The cumulative gas production and hydrogen content in high temperature group were significantly higher than those in medium temperature group. At high temperature of 55 ℃ and pH 6.0, the best dark fermentation effect of food wastes was achieved, and the highest cumulative gas production and hydrogen content were obtained with respective value of 1 100 mL and 73.58%, and the maximum hydrogen production rate and hydrogen production potential were 37.11 mL·h?1 and 660.30 mL, which were followed by kitchen waste, while chicken feces had the worst hydrogen production potential. The concentration of ammonia nitrogen was the highest at the end of hydrogen production by dark fermentation of chicken feces. Excessive concentration of ammonia nitrogen might inhibit the hydrogen production process. VFA analysis showed that the concentration of butyric acid was the highest under different substrates and conditions, and it also contained a small amount of ethanol, acetic acid, propionic acid. The hydrogen production pathway was a mixed fermentation based on butyric acid fermentation. Through optimizing and controlling the non-biological control factors such as temperature and initial pH, the potential of hydrogen production by dark fermentation of agricultural solid wastes and the utilization efficiency of biomass were significantly improved, which provided a theoretical basis for the research and development of biological hydrogen production technology and engineering application.
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