张达1,
韩剑宏1,
姜庆宏1,
李玉梅1,
孙鹏2,
张连科1
1.内蒙古科技大学能源与环境学院，包头 014010
2.华中科技大学环境与市政工程学院，武汉 430074
基金项目: 内蒙古自然科学基金资助项目（2016BS0511）
内蒙古科技大学创新基金（2016QDL-B08）
Leaching properties of thorium in soil by fermentation products of Pseudomonas sp. BS-1
WANG Weida1,,ZHANG Da1,
HAN Jianhong1,
JANG Qinghong1,
LI Yumei1,
SUN Peng2,
ZHANG Lianke1
1.School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China
2.School of Environmental and Municipal Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
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摘要:从焦化废水污泥中筛选出一株产生物表面活性剂的菌株，将其命名为BS-1，16S rDNA系统发育学分析确定其属于假单胞菌属（Pesudomonas sp.）。该菌株发酵产物初步鉴定为鼠李糖脂，在其临界胶束浓度为0.56 g·L-1时，可将水溶液的表面张力从76.8 mN·m-1降至38.5 mN·m-1。将菌株发酵产物溶液作为淋洗剂，采用浸提和土柱淋洗的方法研究了BS-1发酵产物对污染土壤中钍的去除效果，结果表明：当pH为10，浓度为3 g·L-1，振荡时间为72 h时，钍的去除率可达70.14%；在柱状动态实验中，当淋洗量达到800 mL时，发酵产物溶液对钍的累积去除量最大为65.03 mg·kg-1，发酵产物溶液对钍的累积去除量明显优于去离子水。对比淋洗后土壤中钍的形态占比可知，残渣态增加10.16%，氧化结合态减少了11.41%。对比淋洗前后土壤中钍的形态含量可知，酸可提取态、氧化结合态、有机结合态分别减少了83.25%、75.22%、60.70%。淋洗后钍的稳定性增强。
关键词: 假单胞菌属/
鼠李糖脂/
土壤污染修复/
钍
Abstract:A biosurfactant-producing strain BS-1 was isolated from sludge in coking wastewater. It was identified as Pseudomonas (Pesudomonas sp.) according to 16S rDNA phylogenetic analysis. The fermentation product was primarily identified as rhamnolipid, which has high ability to reduce the surface tension when the concentration of critical micelle was 0.56 g·L-1, the surface tension of the aqueous solution can be reduced from 76.8 mN·m-1 to 38.5 mN·m-1.The removal efficiency of thorium from contaminated soil was studied using the fermented product of BS-1 as leaching lotion by elution and column leaching method. The results showed that the maximum removal efficiency can reach 70.14% under the condition of pH was 10, concentration of 3 g·L-1, the oscillating time of 72 h. When leaching amount was achieved 800 mL, the accumulation removal amount of thorium up to 65.03 mg·kg-1 using fermented product of BS-1 as eluting agent and superior to deionized water with column leaching experiment. Contrased the change of fractions of thorium form proportion in the contaminated soil before and after leaching, the residue form increased 10.16% and the oxidation combination form decreased 11.41%. Contrasted the change of fractions of thorium content in the contaminated soil before and after leaching, acid extractable form, oxidation combined form, organic binding state were reduced 83.25%, 75.22%, 60.70%， respectively. The stability of thorium was improved after leaching.
Key words:Pesudomonas sp./
rhamnolipid/
soil remediation/
thorium.
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Pseudomonas sp. BS-1发酵产物对土壤中钍的淋洗作用
王维大1,,张达1,
韩剑宏1,
姜庆宏1,
李玉梅1,
孙鹏2,
张连科1
1.内蒙古科技大学能源与环境学院，包头 014010
2.华中科技大学环境与市政工程学院，武汉 430074
基金项目: 内蒙古自然科学基金资助项目（2016BS0511） 内蒙古科技大学创新基金（2016QDL-B08）
关键词: 假单胞菌属/
鼠李糖脂/
土壤污染修复/
钍
摘要:从焦化废水污泥中筛选出一株产生物表面活性剂的菌株，将其命名为BS-1，16S rDNA系统发育学分析确定其属于假单胞菌属（Pesudomonas sp.）。该菌株发酵产物初步鉴定为鼠李糖脂，在其临界胶束浓度为0.56 g·L-1时，可将水溶液的表面张力从76.8 mN·m-1降至38.5 mN·m-1。将菌株发酵产物溶液作为淋洗剂，采用浸提和土柱淋洗的方法研究了BS-1发酵产物对污染土壤中钍的去除效果，结果表明：当pH为10，浓度为3 g·L-1，振荡时间为72 h时，钍的去除率可达70.14%；在柱状动态实验中，当淋洗量达到800 mL时，发酵产物溶液对钍的累积去除量最大为65.03 mg·kg-1，发酵产物溶液对钍的累积去除量明显优于去离子水。对比淋洗后土壤中钍的形态占比可知，残渣态增加10.16%，氧化结合态减少了11.41%。对比淋洗前后土壤中钍的形态含量可知，酸可提取态、氧化结合态、有机结合态分别减少了83.25%、75.22%、60.70%。淋洗后钍的稳定性增强。
English Abstract
Leaching properties of thorium in soil by fermentation products of Pseudomonas sp. BS-1
WANG Weida1,,ZHANG Da1,
HAN Jianhong1,
JANG Qinghong1,
LI Yumei1,
SUN Peng2,
ZHANG Lianke1
1.School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China
2.School of Environmental and Municipal Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
Keywords: Pesudomonas sp./
rhamnolipid/
soil remediation/
thorium
Abstract:A biosurfactant-producing strain BS-1 was isolated from sludge in coking wastewater. It was identified as Pseudomonas (Pesudomonas sp.) according to 16S rDNA phylogenetic analysis. The fermentation product was primarily identified as rhamnolipid, which has high ability to reduce the surface tension when the concentration of critical micelle was 0.56 g·L-1, the surface tension of the aqueous solution can be reduced from 76.8 mN·m-1 to 38.5 mN·m-1.The removal efficiency of thorium from contaminated soil was studied using the fermented product of BS-1 as leaching lotion by elution and column leaching method. The results showed that the maximum removal efficiency can reach 70.14% under the condition of pH was 10, concentration of 3 g·L-1, the oscillating time of 72 h. When leaching amount was achieved 800 mL, the accumulation removal amount of thorium up to 65.03 mg·kg-1 using fermented product of BS-1 as eluting agent and superior to deionized water with column leaching experiment. Contrased the change of fractions of thorium form proportion in the contaminated soil before and after leaching, the residue form increased 10.16% and the oxidation combination form decreased 11.41%. Contrasted the change of fractions of thorium content in the contaminated soil before and after leaching, acid extractable form, oxidation combined form, organic binding state were reduced 83.25%, 75.22%, 60.70%， respectively. The stability of thorium was improved after leaching.
