中文关键词颗粒污泥短程硝化厌氧氨氧化短程反硝化微生物群落结构 英文关键词granular sludgepartial nitrificationANAMMOXdenitratationstructure of microbial community

作者	单位	E-mail
周锋	华东理工大学资源与环境工程学院, 高浓度难降解有机废水处理技术国家工程实验室, 上海 200237	zf_zfeng@163.com
刘勇弟	华东理工大学资源与环境工程学院, 高浓度难降解有机废水处理技术国家工程实验室, 上海 200237 华东理工大学资源与环境工程学院, 国家环境保护化工过程环境风险评价与控制重点实验室, 上海 200237 上海污染控制与生态安全研究院, 上海 200092
厉巍	华东理工大学资源与环境工程学院, 高浓度难降解有机废水处理技术国家工程实验室, 上海 200237 华东理工大学资源与环境工程学院, 国家环境保护化工过程环境风险评价与控制重点实验室, 上海 200237 上海污染控制与生态安全研究院, 上海 200092	wei_li@ecust.edu.cn

中文摘要 本研究以低碳氮比废水为基质，厌氧氨氧化污泥优配普通活性污泥为接种物，在新型气升式内循环反应器中培育同步短程硝化-厌氧氨氧化-短程反硝化颗粒污泥.结果表明，经过225 d的连续运行可培育成熟稳定的颗粒污泥，其总氮去除率高达91.4%.相较于絮状污泥，颗粒污泥中厌氧氨氧化活性显著增加，并且厌氧氨氧化活性在4个脱氮过程中活性最大，其次是短程硝化，且短程反硝化比活性是亚硝酸盐还原比活性的2.1倍.高通量测序结果表明，颗粒污泥中短程硝化和厌氧氨氧化的优势菌分别为Nitrosomonas和Candidatus_Brocadia，并相较于絮状污泥，它们的丰度分别增加至0.70%和0.57%.Thauera可能是颗粒污泥中潜在的短程反硝化优势菌，其丰度达到0.26%.RT-qPCR分析结果表明，相比接种阶段，短程硝化的功能基因amoA和hao转录水平分别增加了3.5和1.5倍，厌氧氨氧化功能基因hzsA转录水平增加了2.1倍，短程反硝化过程中napA和narG转录水平增加的倍数之和是nirK和nirS的倍数之和的4.8倍.本研究结果将为处理低碳氮比废水提供新的思路. 英文摘要 We cultivated simultaneous partial nitrification, anaerobic ammonium oxidizing(ANAMMOX), and denitratation granular sludge in a novel air-lift internal circulation reactor using low C/N wastewater as the substrate and ANAMMOX sludge matched with ordinary activated sludge as the inoculum. The results showed that the mature and stable granular sludge could be cultivated after 225 d of continuous operation, and the total nitrogen removal rate was as high as 91.4%. Compared with flocculated sludge, the ANAMMOX activity in the granular sludge increased significantly, and the ANAMMOX activity was highest among the four nitrogen removal processes followed by partial nitrification, and the specific denitratation activity was 2.1-times higher than the specific nitrite reduction activity. High-throughput sequencing results showed that the dominant bacteria in partial nitrification and ANAMMOX were Nitrosomonas and Candidatus_Brocadia, respectively, compared to flocculated sludge, with abundances increasing to 0.70% and 0.57%, respectively. Thauera may also be the potential dominant bacteria for denitratation, with an abundance of up to 0.26%. RT-qPCR analysis showed that compared to the inoculation stage, the transcript levels of the amoA and hao genes for partial nitrification increased 3.5-and 1.5-fold, respectively, and the transcript levels of the hzsA gene for ANAMMOX increased 2.1-fold. During denitrataion, the overall abundance of napA and narG transcript levels was 4.8-times higher than that of nirK and nirS. The results of this study provide new insights for the treatment of low C/N wastewater.

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