中文关键词富营养化锁磷剂好氧反硝化菌生物膜水体修复 英文关键词eutrophicationPhoslock^®aerobic denitrifying bacteriumbiofilmwater restoration

作者	单位	E-mail
李炳堂	华中科技大学环境科学与工程学院, 武汉 430074	libingtang@hust.edu.cn
周志勤	华中科技大学环境科学与工程学院, 武汉 430074
Ravi Naidu	Cooperative Research Center for Contamination Assessment and Remediation, University of South Australia Mawson Lakes, Australia
胡智泉	华中科技大学环境科学与工程学院, 武汉 430074	huzq@hust.edu.cn
郭大滨	华中科技大学环境科学与工程学院, 武汉 430074
陈嘉鑫	华中科技大学环境科学与工程学院, 武汉 430074

中文摘要 氮磷是造成水体富营养化的主要原因，单一治理方法常常难以同时有效去除氮磷.本文利用1株分离自富营养化水体的好氧反硝化菌株（AD-19）构建固定化菌膜，并与锁磷剂Phoslock^®联合修复富营养化水体，研究了Phoslock^®的控磷效能和反硝化菌的脱氮性能及二者联合作用时对富营养化水体的修复效果.结果表明，在投加比例为80（Phoslock^®与PO₄^3--P的质量比）的条件下，Phoslock^®对模拟富营养化水体中PO₄^3--P去除率可达95%以上，并能有效抑制底泥中磷的释放.好氧反硝化菌AD-19具有较好的异养硝化-好氧反硝化功能，在以NH₄⁺-N或NO₃^--N为唯一氮源条件下生长良好并能去除97%以上的氮，经16S rDNA鉴定该菌株属于假单胞菌属（Pseudomonas sp.）.利用湖泊模拟装置论证了Phoslock^®联合固定化菌膜修复富营养化水体的可行性，进一步地，利用该技术对武汉市某公园内富营养化池塘进行修复治理，经过16 d的处理，氮磷等水质指标从劣Ⅴ类地表水提升至Ⅲ类，并持续稳定270 d以上，证明Phoslock^®联合固定化菌膜可快速和有效地修复富营养化水体，并保持水质长期稳定. 英文摘要 Nitrogen and phosphorus are the leading causes of water eutrophication, and it is challenging to remove nitrogen and phosphorus effectively through a single water remediation method. In this study, an aerobic denitrifying bacterium (AD-19) isolated from eutrophic water was used to construct an immobilized biofilm and combined with Phoslock^® to remove nitrogen and phosphorus from the water. The phosphorus control efficiency of Phoslock^®, nitrogen removal performance of the denitrifying bacteria, and combined remediation performance for the eutrophic water were studied. The results demonstrated that the removal rate of PO₄^3--P in the simulated eutrophic water reached 95% with a dosing ratio of 80 (mass ratio of Phoslock^® to PO₄^3--P), and phosphorus release from sediment was effectively inhibited at the same time. Strain AD-19, which was identified as Pseudomonas sp. Using the 16S rDNA method, had a good heterotrophic nitrification and aerobic denitrification ability, and more than 97% of the nitrogen was removed when NH₄⁺-N or NO₃^--N was used as the nitrogen source. The feasibility of the combined remediation of the eutrophic water was demonstrated using a lake simulation device. Furthermore, this technique was used to restore a eutrophic pond in a park in Wuhan city. After 16 days of treatment, the water quality indices for nitrogen and phosphorus were improved from worse than Grade Ⅴ to Grade Ⅲ (GB 3838-2002, Ministry of Environmental Protection of China, 2002) and remained stable for more than 270 days, indicating that Phoslock^® combined with the immobilized biofilm could quickly and effectively restore eutrophic water as well as maintain the water quality for long periods.

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