中文关键词厌氧氨氧化耦合反硝化(SAD)脱氮除碳污泥活性微生物群落高通量测序 英文关键词simultaneous ANAMMOX and denitrification (SAD)nitrogen and carbon removalsludge activitymicrobial communityhigh throughput sequencing

作者	单位	E-mail
宋壮壮	重庆大学城市建设与环境工程学院, 重庆 400045 重庆大学三峡库区环境与生态部重点实验室, 重庆 400045	szhzh1013@163.com
吕爽	重庆大学城市建设与环境工程学院, 重庆 400045
刘哲	重庆大学城市建设与环境工程学院, 重庆 400045
时兴东	重庆大学城市建设与环境工程学院, 重庆 400045
潘傲	重庆大学城市建设与环境工程学院, 重庆 400045
张智	重庆大学城市建设与环境工程学院, 重庆 400045	zhangzhicqu@cqu.edu.cn

中文摘要 为了解厌氧氨氧化耦合反硝化启动过程中脱氮除碳性能与微生物群落的关系，通过逐步提高进水COD浓度研究了SAD启动过程中脱氮除碳性能和微生物群落变化.结果表明，随着进水COD浓度增加，出水NH₄⁺-N和NO₂^--N的浓度保持稳定，平均去除率均在98%以上； TN去除率逐渐升高，第3阶段TN平均去除率为95.6%，比厌氧氨氧化理论TN去除率高6.8%；ΔNO₃^--N/ΔNH₄⁺-N明显下降，从0.15~0.17逐步降至0.03~0.07；厌氧氨氧化脱氮贡献率逐渐下降，反硝化脱氮贡献率逐渐上升，COD去除率逐步增加.污泥活性分析表明SAD启动后污泥反硝化活性明显增加，厌氧氨氧化活性略微降低.高通量测序结果表明，反应器内微生物的优势菌门为绿弯菌门、浮霉菌门、厚壁菌门、装甲菌门和变形菌门，微生物群落特征与SAD脱氮除碳性能密切相关，与脱氮除碳有关的功能微生物主要有厌氧氨氧化菌、厌氧消化菌和反硝化菌，SAD启动后反应器内厌氧氨氧化菌丰度减少，厌氧消化菌和反硝化菌丰度明显增加. 英文摘要 To understand the relationship between nitrogen and carbon removal performance and the microbial community during start-up of simultaneous ANAMMOX and denitrification (SAD), nitrogen and carbon removal performance and microbial community change were studied by gradually increasing the influent COD concentration. The results showed that with the increase of the influent COD concentration, NH₄⁺-N and NO₂^--N effluent remained stable, and the average removal rate was more than 98%. The removal rate of TN increased gradually, and the rate was 95.6% in the third stage, which was 6.8% higher than that of ANAMMOX in theory. ΔNO₃^--N/ΔNH₄⁺-N decreased significantly from 0.15-0.17 to 0.03-0.07. The contribution rate of ANAMMOX to nitrogen removal decreased gradually, denitrification for nitrogen removal increased gradually, and the COD removal rate increased. Sludge activity analysis showed that the denitrification activity of sludge increased significantly and the ANAMMOX activity decreased slightly after the start-up of SAD. High throughput sequencing results showed that the dominant phyla of microorganisms in the reactor were Chloroflexi, Planctomycetes, Firmicutes, Armatimonadetes, and Proteobacteria. The characteristics of the microbial community in the reactor were closely related to the performance of SAD in nitrogen and carbon removal. The main functional microorganisms related to nitrogen and carbon removal were ANAMMOX bacteria, anaerobic digestive bacteria, and denitrifying bacteria. The abundance of ANAMMOX bacteria decreased after the start-up of SAD, and the anaerobic digestive bacteria and heterotrophic denitrifying bacteria increased significantly.

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