中文关键词增温秸秆施用微生物量碳氮细菌群落结构 英文关键词warmingstraw applicationmicrobial biomass carbon and nitrogenbacteriacommunity structure

作者	单位	E-mail
张婷婷	南京信息工程大学江苏省大气环境与装备技术协同创新中心, 南京 210044 南京信息工程大学应用气象学院, 南京 210044	iamedith@foxmail.com
陈书涛	南京信息工程大学江苏省大气环境与装备技术协同创新中心, 南京 210044 南京信息工程大学应用气象学院, 南京 210044	chenstyf@aliyun.com
王君	南京信息工程大学应用气象学院, 南京 210044
王朝辉	南京信息工程大学应用气象学院, 南京 210044
胡正华	南京信息工程大学应用气象学院, 南京 210044

中文摘要 为研究增温和秸秆施用对农田土壤微生物量碳氮和细菌群落结构的影响，设置随机区组试验，试验设置空白（CK）、增温（WA）、秸秆施用（SA）和增温及秸秆施用（WS）这4种处理.分别在大豆生长季和冬小麦生长季采集土壤样品，测定土壤微生物量碳氮，并采用Illumina HiSeq高通量测序法测定土壤细菌群落结构.结果表明，无论是大豆季还是冬小麦田，不同增温及秸秆施用处理的土壤微生物量碳含量均无显著（P>0.05）差异，大豆季增温处理土壤微生物量氮含量显著（P<0.01）高于对照，且大豆季土壤微生物量氮含量极显著（P<0.001）高于冬小麦田.大豆季各处理间土壤细菌纲、目、科和属水平上的类群均无显著（P>0.05）差异，冬小麦季不同处理间的细菌群落结构存在显著差异.大豆季CK与WA、SA处理最具优势的芽单胞菌目（Gemmatimonadales）、芽单胞菌科（Gemmatimonadaceae）和鞘氨醇单胞菌属（Sphingomonas）的细菌比例之间均存在显著（P<0.05）差异，冬小麦季CK与WA处理最具优势的γ-变形菌纲（γ-Proteobacteria）的细菌比例之间存在显著（P<0.05）差异.大豆季增温处理的物种数、Shannon指数、Simpson指数和Chao1指数均最小，冬小麦季增温及秸秆施用处理的物种数、Shannon指数、Simpson指数和Chao1指数均最大.大豆季土壤细菌α多样性指标与土壤微生物量碳氮之间存在极显著（P<0.001）的相关关系，而冬小麦季这些指标之间则不存在类似的关系. 英文摘要 In order to investigate the effects of warming and straw application on soil microbial biomass carbon and nitrogen and bacterial community structure, a randomized block experiment was performed. Four treatments were included, namely a control (CK), warming (WA), straw application (SA), and warming and straw application (WS) treatments. The soils were sampled during the soybean (Sep. 23, 2017) and winter wheat (April 21, 2018) growing seasons. The soils were used to determine the microbial biomass C and N content using chloroform fumigation methods, and the bacterial community structure was evaluated using high-throughput sequencing (Illumina HiSeq). Results indicated that there was no significant difference in microbial biomass C between different warming and straw application treatments (P>0.05). The microbial biomass N of the warming treatment was significantly higher than that of control in the soybean field (P<0.01). There were significant differences in the most dominant soil bacteria between treatments in the soybean growing season (P<0.05) at the class, order, family, and genus levels, while there was no difference in the winter wheat growing season. The percentages of dominant Gemmatimonadales, Gemmatimonadaceae, and Sphingomonas in the CK and WA (or SA) treatments were significantly different (P<0.05) in the soybean growing season. There was a significant (P<0.05) difference in the dominant Gammaproteobacteria between the CK and WA treatments in the winter wheat growing season. The observed number of species, Shannon index, Simpson index, and Chao1 index were lowest in the warming plots in the soybean growing season and highest in the warming and straw application plots in the winter wheat growing season. The Shannon index for the WA plots was significantly higher than in the WS plots in the soybean growing season (P<0.05). The observed number of species, Shannon index, Simpson index, and Chao1 index were significantly higher in the soybean plots than in the winter wheat plots (P<0.05), while the abundance was significantly higher in the winter wheat plots than in the soybean plots (P<0.05). The soybean growing season had significantly higher diversity than the winter wheat growing season. The indexes of α diversity were highly significantly correlated with soil microbial biomass C and N in the soybean growing season (P<0.001), while there was no such correlation in the winter wheat growing season. The indices of α diversity were significantly correlated in both the soybean growing season and winter wheat growing season (P<0.05).

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