刘凯1,2,
孙一鑫1,
王孝天1,
方涛1,3,4,
吴亚平1,3,4,
冯志华1,3,4,,
1. 江苏海洋大学江苏省海洋生物资源与环境重点实验室, 连云港 222005;
2. 华东师范大学河口海岸学国家重点实验室, 上海 200241;
3. 江苏海洋大学江苏省海洋生物技术重点实验室, 连云港 222005;
4. 江苏海洋大学江苏省海洋生物产业技术协同创新中心, 连云港 222005
作者简介: 张涛(1988-),男,讲师,博士,硕士生导师,研究方向为海洋生物地球化学,E-mail:zhangtao@jou.edu.cn.
通讯作者: 冯志华,fengzhihua@jou.edu.cn
基金项目: 江苏省产学研合作项目（BY2020428）；江苏省高等学校自然科学研究项目（20KJB170029）；江苏省海洋科学与技术优势学科建设工程资助项目中图分类号: X171.5
Effects of Microplastics on Physiological Metabolism of Perinereis aibuhitensis
Zhang Tao1,3,4,Liu Kai1,2,
Sun Yixin1,
Wang Xiaotian1,
Fang Tao1,3,4,
Wu Yaping1,3,4,
Feng Zhihua1,3,4,,
1. Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China;
2. State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200241, China;
3. Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China;
4. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005, China
Corresponding author: Feng Zhihua,fengzhihua@jou.edu.cn
CLC number: X171.5
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摘要:为探讨微塑料对双齿围沙蚕生理代谢的影响，本文研究了聚丙烯（PP）、聚酰胺（PA）和聚氯乙烯（PVC）3种微塑料在不同浓度（0.1%、1%和5%）和暴露时间下对双齿围沙蚕体内过氧化氢酶（CAT）、乙酰胆碱酯酶（AchE）活性以及代谢指标排氨率和呼吸速率的影响。结果表明，随微塑料浓度的升高，双齿围沙蚕体内CAT活性显著提升，5%浓度下双齿围沙蚕体内CAT活性最高；PVC对双齿围沙蚕体内AchE活性表现为低浓度（0.1%）抑制、高浓度（5%）促进的作用；PP和PA在不同浓度下对双齿围沙蚕体内AchE酶活性均表现为促进作用；随着微塑料浓度的升高，双齿围沙蚕的排氨率和呼吸速率显著升高（P<0.05）。综上，微塑料在高浓度条件下，在一定时间内可以对双齿围沙蚕产生氧化胁迫作用，并一定程度上影响其排氨率和呼吸速率。
关键词: 微塑料/
双齿围沙蚕/
抗氧化/
代谢/
过氧化氢酶/
乙酰胆碱酯酶/
排氨率/
呼吸速率
Abstract:In order to investigate the effect of microplastics on the physiological metabolism of the bidentate silkworm, this paper studied the effect of three microplastics of polypropylene (PP), polyamide (PA) and polyvinyl chloride (PVC) at different concentrations (0.1%, 1%, 5%) and exposure time on the activities of catalase (CAT) and acetylcholinesterase (AchE), as well as the metabolic index ammonia excretion rate and respiration rate. The results show that the CAT activity of P. aibuhitensis increased significantly with the increase of microplastics concentration. The CAT activity of P. aibuhitensis is the highest under 5% microplastic concentration. The effect of PVC on the AchE activity of P. aibuhitensis is inhibited by low concentration (0.1%) and promoted by high concentration (5%). Different concentrations of PP and PA can promote the AchE activity of P. aibuhitensis. With the increase of microplastics concentration, the ammonia excretion rate and respiration rate of P. aibuhitensis increased significantly (P<0.05). In summary, the microplastics can produce oxidative stress on P. aibuhitensis within a certain period of time under high concentration, and affect its ammonia excretion rate and respiration rate to a certain extent.
Key words:microplastics/
Perinereis aibuhitensis/
anti-oxidation/
metabolism/
catalase/
acetylcholinesterase/
ammonia excretion rate/
respiration rate.
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