叶梦婷,
傅诗雨,
岑绮雯,
吴琼,
胡英超,
陆洪良^,
杭州师范大学生命与环境科学学院生态系统保护与恢复杭州市重点实验室, 杭州 310036

作者简介: 叶梦婷(1997-),女,学士,研究方向为生态毒理学,E-mail:995171074@qq.com.

通讯作者: 陆洪良,honglianglu@outlook.com

基金项目: 浙江省自然科学基金项目(LY15C030006)


中图分类号: X171.5

Effects of Glyphosate-ammonium Exposure on Hatchling Phenotypes and Physiological Performances in the Chinese Soft-shelled Turtle during the Embryonic Stage

Ye Mengting,
Fu Shiyu,
Cen Qiwen,
Wu Qiong,
Hu Yingchao,
Lu Hongliang^,
Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China

Corresponding author: Lu Hongliang,honglianglu@outlook.com

CLC number: X171.5

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摘要:草甘膦是全球范围内生产与使用量最大的除草剂。虽然草甘膦制剂被分于低毒等级,但是许多研究显示其对动物的生理、行为表达具有广泛的干扰作用。本研究将中华鳖胚胎暴露于不同浓度的草甘膦铵盐溶液以评估草甘膦除草剂对其胚胎发育及孵出幼体表型及生理表现的影响。结果显示:一定浓度范围(5~5 000 mg·L^-1)内,草甘膦铵盐对中华鳖胚胎发育速率、孵出幼体大小、游泳能力、肝脏甘油三酯含量、超氧化物歧化酶活性、丙二醛含量以及热激蛋白70 mRNA相对表达量无显著影响。结果表明:一定浓度范围草甘膦除草剂暴露对中华鳖胚胎发育的影响较小,不改变孵出幼体的表型及其生理表现。
关键词: 草甘膦/
中华鳖/
超氧化物歧化酶活性/
丙二醛含量/
热激蛋白70表达水平

Abstract:Many studies have shown that glyphosate and its commercial formulations have widespread effects on the physiological and behavioral performances of animals, despite being classified as low-level toxicity. In aquatic vertebrates, toxicity evaluation of glyphosate mainly focuses on fish and amphibians; however, knowledge of detrimental effects in reptiles is very limited. Here, we exposed eggs of Chinese soft-shelled turtle (Pelodiscus sinensis) to five different concentration treatments (0, 5, 50, 500 and 5 000 mg·L^-1) of glyphosate-ammonium and measured the locomotor performance, activity level, and some hepatic physiological indicators (triglyceride level, superoxide dismutase activity, malonaldehyde content, and relative mRNA expression of heat shock protein 70) of hatchlings to assess potential impacts of embryonic exposure to glyphosate herbicide on embryonic development and hatchling phenotypes and physiological performances in this species. Within the range of 5-5 000 mg·L^-1, glyphosate-ammonium exposure did not affect the hatching success and incubation period of P. sinensis eggs. Except for activity level, other examined hatchling traits could not be affected by glyphosate-ammonium exposure, possibly due to the low toxicity of glyphosate.
Key words:glyphosate-ammonium/
Pelodiscus sinensis/
superoxide dismutase/
malonaldehyde/
heat shock protein 70 expression.