陈香平¹,
黄长江²,
陈元红²,
刘滨³,
刘翠平³,
朱亚先⁴,
张勇¹
1. 近海海洋环境科学国家重点实验室(厦门大学), 厦门大学环境与生态学院, 厦门 361102;
2. 温州医科大学公共卫生与管理学院, 温州 325035;
3. 温州出入境检验检疫局, 温州 325027;
4. 厦门大学化学化工学院化学系, 厦门 361102

作者简介: 陈香平(1985-),女,博士研究生,研究方向为水生生态毒理学,E-mail:pxc850222@163.com.

基金项目: 国家自然科学基金项目(21577110)；高等学校博士学科点专项科研基金(20130121130005)


中图分类号: X171.5

Neurotoxic Effects of Co-exposure to Lead and Dechlorane Plus on Zebrafish (Danio rerio) Embryos

Chen Xiangping¹,
Huang Changjiang²,
Chen Yuanhong²,
Liu Bin³,
Liu Cuiping³,
Zhu Yaxian⁴,
Zhang Yong¹
1. State Key Laboratory of Marine Environmental Science of China(Xiamen University), College of the Environment and Ecology, Xiamen University, Xiamen 361102, China;
2. College of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, China;
3. Wenzhou Entry Exit Inspection and Quarantine Bureau, Wenzhou 325027, China;
4. Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

CLC number: X171.5

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摘要:以斑马鱼(Danio rerio)为研究对象,探讨铅(Pb)、得克隆(DP)及二者联合急性暴露对斑马鱼胚胎的神经毒性作用。结果表明,Pb(5、20 μg·L^-1)和DP(15、60 μg·L^-1)单独暴露均会引起斑马鱼自主运动频率增加,触摸反应能力和自由游泳活力下降,并且抑制初级运动神经元的生长,加剧尾部细胞凋亡。但与20 μg·L^-1 Pb单独暴露相比,高剂量联合暴露(20 μg·L^-1 Pb + 60 μg·L^-1 DP)使斑马鱼的自主运动频率显著降低(P < 0.05),触摸反应能力和自由游泳活力显著增强(P < 0.05),初级运动神经元轴突长度显著增加(P < 0.05),尾部细胞凋亡减少。与5 μg·L^-1 Pb单独暴露相比,低剂量联合暴露(5 μg·L^-1 Pb + 15 μg·L^-1 DP)也显著减少斑马鱼尾部的细胞凋亡(P < 0.05)。上述结果表明,Pb或DP单独暴露对斑马鱼均可引起神经毒性作用;但二者联合暴露对斑马鱼自主运动、触摸反应以及自由游泳活力的影响则表现为拮抗作用。
关键词: 铅/
得克隆/
斑马鱼/
胚胎/
神经毒性/
联合暴露

Abstract:Neurotoxic effects of acute exposure to lead (Pb) or Dechlorane Plus (DP), or both were investigated using zebrafish (Danio rerio) embryos. Results showed that exposure to Pb (5, 20 μg·L^-1) or DP (15, 60 μg·L^-1) alone increased spontaneous movement, decreased touch response and free-swimming activity, inhibited axonal growth of primary motoneuron and induced cell apoptosis in zebrafish embryos. Co-exposure to 20 μg·L^-1 Pb and 60 μg·L^-1 DP significantly decreased spontaneous movement (P < 0.05), enhanced touch response and free-swimming activity (P < 0.05), increased axonal length of primary motoneuron (P < 0.05) and reduced cell apoptosis in zebrafish when compared to 20 μg·L^-1 Pb exposure alone. Co-exposure to 5 μg·L^-1 Pb and 15 μg·L^-1 DP also significantly decreased cell apoptosis on the tail region when compared to 5 μg·L^-1 Pb exposure alone (P < 0.05). These results demonstrated that Pb or DP exposure alone could induce neurobehavioral toxicity in zebrafish, but Pb and DP co-exposure had antagonistic effects on spontaneous movements, touch response and free swimming activity.
Key words:lead/
Dechlorane Plus/
zebrafish/
embryo/
neurotoxicity/
co-exposure.