池振兴^1,2,
王东麟^1,3,
凌嘉佳¹,
宋雪梅⁴,
王明静⁴
1. 哈尔滨工业大学(威海)海洋科学与技术学院, 威海 264209;
2. 暨南大学环境学院 广州市环境暴露与健康重点实验室, 广州 510632;
3. 清华大学环境学院 环境模拟与污染控制国家重点联合实验室, 北京 100084;
4. 威海市中心血站, 威海 264200

作者简介: 池振兴(1983-),男,博士,研究方向为环境污染与健康,E-mail:zhenxingchi@gmail.com.

基金项目: 国家自然科学基金(21707026)；广州市环境暴露与健康重点实验室开放基金(GZKLEEH201613)


中图分类号: X171.5

Distribution and Level of Dimethyl Phthalate in Erythrocytes in Vitro

Chi Zhenxing^1,2,
Wang Donglin^1,3,
Ling Jiajia¹,
Song Xuemei⁴,
Wang Mingjing⁴
1. School of Marine Science and Technology, Harbin Institute of Technology, Weihai, Weihai 264209, China;
2. Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, Guangzhou 510632, China;
3. State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China;
4. Weihai Blood Center, Weihai 264200, China

CLC number: X171.5

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摘要:邻苯二甲酸酯类污染物(PAEs)在环境中普遍存在,可沿食物链富集,危害人体健康。本文利用荧光光谱法和高效液相色谱法(HPLC)探究了邻苯二甲酸二甲酯(DMP)在离体人红细胞内的分布情况。结果表明,红细胞在310 nm、490 nm和609 nm处各具有一个荧光特征峰,其来源分别为:红细胞内的蛋白;还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)、黄素腺嘌呤二核苷酸(FAD)和膜脂;锌卟啉和原卟啉。DMP染毒后,310 nm处的荧光峰出现明显下降,其原因为进入红细胞内的DMP与蛋白发生了结合;490 nm和609 nm处的荧光峰变化很小。高效液相色谱(HPLC)实验结果表明,DMP能透过红细胞膜进入细胞内部,其进入量随暴露量增加而增加,进入量和暴露量的比值随暴露量增加而减少。上述研究成果能加深对PAEs在血液运输过程中与红细胞毒性作用的理解,可为PAEs的危险性评估和相关疾病预防提供数据参考。
关键词: 邻苯二甲酸酯/
红细胞/
污染物分布/
荧光光谱/
高效液相色谱

Abstract:Phthalate esters (PAEs) is widely present in the environment. It poses a threat to human health through accumulation in the food chain. The distribution of a typical PAEs, i.e., dimethyl phthalate (DMP), in human erythrocytes in vitro was explored by using fluorescence spectroscopy and HPLC. The experimental results indicated that the fluorescence spectrum of erythrocytes have three fluorescence peaks, successively located at 310 nm, 490 nm and 609 nm, which are caused by the protein in erythrocytes; nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD) and membrane lipid; zinc porphyrin and porphyrin, respectively. After exposure to DMP, the intensity of the fluorescence peak at 310 nm significantly decreased due to the binding of DMP with the protein in erythrocytes, while no significant change in the intensity of the fluorescence peaks at 490 nm and 609 nm was observed. The experimental results of HPLC indicated that DMP could pass through the cell membrane and enter the cell. The DMP level in the cell increased with the increasing exposure level. The ratio of the DMP level in the cell to the exposure level decreased with increasing exposure level. The results will shed light on the understanding of the cytotoxicity of PAEs in the process of blood transport.
Key words:phthalate esters (PAEs)/
erythrocyte/
pollutant distribution/
fluorescence spectroscopy/
HPLC.