侯天芳¹,
王广发²,
陈乾华^1,,
1. 航空总医院呼吸科, 北京 100012;
2. 北京大学第一医院呼吸和危重症科, 北京 100034

作者简介: 侯天芳(1988-),女,博士研究生,研究方向为大气污染与气道疾病,E-mail:tf_hou@126.com.

通讯作者: 陈乾华,hkzyy0150@163.com

基金项目: 北京市自然科学基金重点项目(No.7161013)


中图分类号: X171.5

Effect of Fine Particulate Matter on T Lymphocyte Subsets in Mice

Hou Tianfang¹,
Wang Guangfa²,
Chen Qianhua^1,,
1. Department of Respiratory of Aviation General Hospital, Beijing 100012, China;
2. Department of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing 100034, China

Corresponding author: Chen Qianhua,hkzyy0150@163.com

CLC number: X171.5

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摘要:大气颗粒物对肺免疫系统有潜在毒性作用,打破免疫系统平衡,大气颗粒物成分中危害首当其冲的是大气细颗粒物(PM_2.5),为研究大气细颗粒物引起的机体T淋巴细胞中Th1/Th2免疫失衡方向。本研究通过免疫组化实验方法检测暴露后小鼠肺组织中T淋巴细胞的表达,进一步采用流式细胞术检测大气细颗粒物气管滴注后小鼠肺脏淋巴细胞中Th1/Th2比例。暴露组小鼠肺组织免疫组化研究结果提示浸润细胞区有大量的CD4⁺ T细胞,中高剂量暴露组小鼠肺组织中淋巴细胞亚群Th1/Th2比例向Th1偏移。大气细颗粒物影响免疫失衡,使T淋巴细胞向Th1漂移。
关键词: 大气细颗粒物/
CD4⁺ T细胞/
免疫失衡/
流式细胞术

Abstract:Atmospheric particulate matter have potential toxic effects on the lung immune system, which break the balance of the immune system. The most harmful component of atmospheric pollution is atmospheric fine particulate matter (PM_2.5). To explore the imbalance of Th1/Th2 differentiation triggered by PM_2.5, the expression of T lymphocytes in lung tissue of mice was detected by immunohistochemistry, then flow cytometry was used to detect the proportion of Th1/Th2 in lung of mice after an intratracheal instillation of PM_2.5. The immune histochemistry of lung tissue showed that there are a large number of CD4⁺ T cells in the infiltrating cell area, and the Th1 shifting in lung of mice was observed. The exposure to PM_2.5 affects the immune balance, driving a Th1-biased immune response.
Key words:atmospheric particulate matter/
CD4⁺ T cells/
immune imbalance/
flow cytometry.