唐天乐^1,2,
杨洋^2,3,
陈亚文^2,3,
朱小乔^2,3,
唐文浩^2,3,
刁晓平^2,3
1. 海南医学院热带医学与检验医学院, 海口 571199;
2. 海南大学环境与植物保护学院, 海口 570228;
3. 海南大学海口市环境毒理学重点实验室, 海口 570228

作者简介: 唐天乐(1985-),男,讲师,在读博士研究生,研究方向为环境毒理学,E-mail:ttl-0114@163.com.

基金项目: 海南省科协青年科技人才学术创新计划项目(No.201513)；海南医学院科研培育基金(No.HY2014-013)


中图分类号: X171.5

Effects of BPAF on Thyroid Tissue and Thyroid Hormones of Male Zebrafish (Danio rerio)

Tang Tianle^1,2,
Yang Yang^2,3,
Chen Yawen^2,3,
Zhu Xiaoqiao^2,3,
Tang Wenhao^2,3,
Diao Xiaoping^2,3
1. School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou 571199, China;
2. College of Environment and Plant Protection, Hainan University, Haikou 570228, China;
3. Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou 570228, China

CLC number: X171.5

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摘要:为评价双酚AF(BPAF)的甲状腺毒性，以雄性斑马鱼成鱼为受试模式生物，采用半静态染毒的方式对雄性斑马鱼成鱼染毒14 d(0、5、50和500 μg·L^-1)，分别设置7 d、14 d 2个时间节点，通过酶联免疫法(ELISA)分别检测斑马鱼血浆中总三碘甲腺原氨酸(TT3)、总甲状腺素(TT4)、游离三碘甲腺原氨酸(FT3)、游离甲状腺素(FT4)的含量水平，并对14 d暴露后斑马鱼甲状腺进行组织学检测分析。结果显示随着暴露浓度的提高，出现甲状腺滤泡上皮细胞增生，滤泡组织变形明显，细胞胶质缺失等病理学变化。雄性斑马鱼在BPAF暴露情况下，血浆中TT3、TT4、FT3、FT4含量随着暴露浓度的提升和时间的延长出现显著上升，呈现出甲状腺功能亢奋的干扰效应。
关键词: 双酚AF/
斑马鱼/
甲状腺组织/
甲状腺激素水平

Abstract:In order to evaluate the endocrine toxicity of BPAF, the male zebrafish (Danio rerio) was used as a target animal and exposed to 0, 5, 50 and 500 μg·L^-1 BPAF for 14 days by way of semi static exposure. In this study, the concentrations of total 3,3',5-triiodothyronine (TT3), total 3,5,3',5'-tetraiodothyronine (TT4), free 3,3',5-triiodothyronine (FT3), free 3,5,3',5'-tetraiodothyronine (FT4) in plasma of zebrafish were detected by ELISA after exposure for 7 days and 14 days. The thyroid gland of zebrafish was analyzed by histological techniques after exposure for 14 days. The results showed increased number of epithelial cells, cell colloid deficiency and obvious follicle tissue deformation in the thyroid with the increase of BPAF exposure concentration. The concentration of thyroid hormones (THs) in plasma of male zebrafish increased significantly with the increase of BPAF exposure concentration and time. It showed the interference effect of hyperthyroidism.
Key words:BPAF/
zebrafish/
thyroid tissue/
thyroid hormone.