包小丹,
李天函,
郭梦龙,
杨威利,
李耀华,
王文锋,
井长勤
新乡医学院生命科学技术学院, 新乡 453003

作者简介: 包小丹(1991-),女,硕士研究生,研究方向为植物生态毒理学,E-mail:1057735318@qq.com.

基金项目: 河南省科技攻关项目(142102310045，152102210126)


中图分类号: X171.5

Genotoxic Effects of Ionic Liquid Chloride 1-Octyl-3-methylimidazolium on Onions

Bao Xiaodan,
Li Tianhan,
Guo Menglong,
Yang Weili,
Li Yaohua,
Wang Wenfeng,
Jing Changqin
College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China

CLC number: X171.5

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摘要:为探讨咪唑类离子液体氯化1-辛基-3-甲基咪唑([C₈mim][C1])对洋葱的遗传毒性，用浓度分别为2.5、5、10和20 mmo·L^-1的[C₈mim][C1]处理洋葱内表皮细胞和洋葱根尖8 h，用浓度为10 mmo·L^-1的[C₈mim][C1]处理洋葱内表皮细胞和洋葱根尖4 h、8 h、12 h和24 h，研究[C₈mim][C1]对洋葱内表皮细胞核的影响和对洋葱根尖有丝分裂指数和染色体畸变的影响。结果显示，与对照组相比，随着[C₈mim][Cl]处理浓度和时间的增加，洋葱内表皮细胞核偏移率明显升高，根尖细胞有丝分裂指数显著降低，染色体畸变现象明显，畸变率升高。研究表明，当浓度达到 2.5 mmo·L^-1时，[C₈mim][Cl]对洋葱具有遗传毒性。
关键词: [C₈mim][Cl]/
洋葱/
遗传毒性

Abstract:The aim of this study was to explore the genotoxicity of imidazole ionic liquid chloride 1-octyl-3-methylimidazolium ([C₈mim][C1]). Endothelial cells (EC) and root tip cells (RTC) of onion were treated with [C₈mim][C1] at the variable concentrations for 8 hours (2.5, 5, 10 and 20 mmo·L^-1). Cells were also treated with [C₈mim][C1] at the concentration of 10 mmo·L^-1 for different time period of 4, 8, 12 and 24 hours, respectively. The effect of [C₈mim][C1] on onion nucleus was observed, mitotic index was calculated and chromosome aberration of RTC were detected after the treatment of [C₈mim][C1]. The results showed that [C₈mim][Cl] exposure induced the location change of onion epidermal cell nucleus. In addition, [C₈mim][Cl] caused the decrease of mitotic index of RTC and the increase of chromosome aberration ratio. These results demonstrated that the genotoxicity of [C₈mim][Cl] on EC and RTC is in a dose- and time-dependent manner when the [C₈mim][Cl] concentration reaches 2.5 mmo·L^-1.
Key words:[C₈mim] [Cl]/
onion/
genotoxicity.