刘瑞祥^1,2,
边高鹏^1,2,
兰婷婷¹,
孙学茹¹,
常惠丽³,
任嘉红^1,2
1. 长治学院生物科学与技术系, 长治 046011;
2. 太行山生态与环境研究所, 长治 046011;
3. 长治市环境监测站, 长治 046000

作者简介: 刘瑞祥(1964-),男,汉族,硕士,教授,研究方向为遗传毒理学,E-mail:liuruixiang1964@163.com.

基金项目: 山西省高等学校大学生创新创业训练重点项目(No.2015426)；地方高校国家级大学生创新创业训练计划项目(No.201510122001)


中图分类号: X171.5

Genotoxicity Evaluation of Organic Pollutants in Ground Water with Comet Assay

Liu Ruixiang^1,2,
Bian Gaopeng^1,2,
Lan Tingting¹,
Sun Xueru¹,
Chang Huili³,
Ren Jiahong^1,2
1. The Department of Biology Science and Technology, Changzhi College, Changzhi 046011, China;
2. Taihang Mountain Institute of Ecology and Environment, Changzhi 046011, China;
3. Environmental Monitoring Station of Changzhi, Changzhi 046000, China

CLC number: X171.5

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摘要:应用人外周血淋巴细胞和小鼠淋巴细胞彗星试验,对长治市5个样点的地下水有机浓集物进行了遗传毒性研究。结果表明,各水样中有机浓集物在3个染毒剂量50,200,800 mL 管^-1条件下均可对人外周血淋巴细胞和小鼠淋巴细胞DNA产生不同程度的遗传损伤,并且随着浓度的升高,DNA损伤程度明显增加,各实验组与溶剂对照组相比有P < 0.05或P < 0.01显著差异。研究表明,上述2种淋巴细胞彗星试验可有效地检测地下水体有机污染物的遗传毒性。
关键词: 地下水/
有机污染物/
彗星试验/
人外周血淋巴细胞/
小鼠淋巴细胞

Abstract:The genotoxicity of organic pollutants to human peripheral lymphocytes and mouse lymphocytes in Changzhi groundwater was investigated. The comet assay was conducted to detect DNA damage induced by the organic extracts of five typical sample sites. The results showed that the DNA damage in human peripheral lymphocytes and mouse lymphocytes was induced by the organic substances tested in 50，200 and 800 mL tube^-1. DNA damage increased obviously with the dose of the organic extracts. Significant differences (P< 0.05, P< 0.01) were observed compared with the negative control group. This study also demonstrated that comet assay could be successfully applied to the genotoxicity monitoring of water organic extracts in human peripheral lymphocytes and mouse lymphocytes.
Key words:ground water/
organic pollutants/
comet assay/
human peripheral lymphocytes/
mouse lymphocytes.