赵旭,
叶幸,
张燕,
宋伟华,
姜辉,
瞿唯钢
农业部农药检定所, 北京 100125

作者简介: 赵旭(1991-),男,农学学士,研究方向为农药环境毒理,E-mail:1414850890@qq.com.

基金项目: 农业部以《农业部办公厅关于下达2016年农业行业标准制订和修订及农产品质量认证项目的通知》(农办质[2016]28号)下达的《微生物农药环境风险评价试验准则 家蚕毒性试验》制定项目


中图分类号: X171.5

Study on the Toxicity of Three Typical Microbial Pesticides on Silkworm

Zhao Xu,
Ye Xing,
Zhang Yan,
Song Weihua,
Jiang Hui,
Qu Weigang
Institute for the Control of Agrochemicals, Ministry of Agriculture, Beijing 100125, China

CLC number: X171.5

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摘要:苏云金芽孢杆菌、球孢白僵菌以及棉铃虫核型多角体病毒是细菌类、真菌类和病毒类微生物农药的典型代表。为了明确微生物农药对家蚕的毒性影响，选取以上3种典型微生物农药，分别探究其对家蚕的毒性。结果显示：苏云金杆菌原药对家蚕LC₅₀值为1.33×10⁵ CFU·mL^-1；球孢白僵菌原药对家蚕LC₅₀值为1.14×10⁶ CFU·mL^-1；棉铃虫核型多角体病毒母药对家蚕LC₅₀值大于1.00×10⁸ PIB·mL^-1。与此同时，通过进一步试验，明确了以上3种微生物农药对家蚕结茧率、死笼率、全茧量、茧层量等关键指标的影响。结果显示：除了棉铃虫核型多角体病毒母药，高浓度苏云金杆菌原药、球孢白僵菌原药对家蚕的结茧率和死笼率均具有显著影响；3种微生物农药对家蚕产茧能力指标全茧量、茧层量以及蛹重均无显著性影响。
关键词: 微生物农药/
家蚕/
毒性

Abstract:Bacillus thuriengiensis (Bt), Beauveria bassiana (Bb) and Heliothis armigera nuclear polyhedrosis viruses (HaNPV) are typical microbial pesticides of bacterium, fungus and virus. In order to identify the toxicity of microbial pesticides to silkworm, this study measured the toxicity of these three typical microbial pesticides to silkworm, Bombyx mori L.. The results showed that LC₅₀ of the Bt technical and Bb technical to 2nd instar larvae of silkworm are 1.33×10⁵ CFU·mL^-1, 1.14×10⁶ CFU·mL⁵, respectively; LC₅₀ of HaNPV technical concentrates to 2nd instar larvae of silkworm is higher than 1.00×10⁸ PIB·mL^-1. At the same time, some key indicators, such as cocooning rate, the rate of dead worm cocoon, cocoon weight, cocoon shell weight, are detected through further experiment. The result showed that except HaNPV technical concentrates，both high concentration of Bt technical and Bb technical have significant influence on cocooning rate and the rate of dead worm cocoon of silkworm. However, the three typical microbial pesticides have no significant influence on cocoon weight, cocoon shell weight and pupal weight.
Key words:microbial pesticides/
silkworm/
toxicity.