江媛媛^1,2,
党菲²,
李敏²,
周东美²,
吴笛³,
施维林¹,
朱雪峰⁴
1. 苏州科技大学环境科学与工程学院, 苏州 215009;
2. 中国科学院南京土壤研究所土壤环境与污染修复重点实验室, 南京 210008;
3. 南京医科大学公共卫生学院毒理系, 南京 210029;
4. 南京医科大学公共卫生学院江苏省医药兽药安全性评价与研究中心, 南京 210029

作者简介: 江媛媛(1990-),女,硕士研究生,研究方向为生态毒理效应,E-mail:syjiangyuanyuan@163.com.

基金项目: 国家自然科学基金项目(No. 41430752, 31570515)；江苏省自然科学基金项目(BK20131041)；苏州市科技支撑计划项目(SS201421，SS201523)；江苏高校水处理技术与材料协同创新项目；苏州市环保科技项目(2015-3)


中图分类号: X171.5

Bioavailability and Distribution of Nanosilver after Oral Administration in Rats

Jiang Yuanyuan^1,2,
Dang Fei²,
Li Min²,
Zhou Dongmei²,
Wu Di³,
Shi Weilin¹,
Zhu Xuefeng⁴
1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;
2. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;
3. Department of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, China;
4. Safety Evaluation and Research Center of Medicine and Veterinary Medicine of Jiangsu Province, School of Public Health, Nanjing Medical University, Nanjing 210029, China

CLC number: X171.5

-->

摘要
HTML全文
图(0)表(0)
参考文献(0)
相关文章
施引文献
资源附件(0)
访问统计

摘要:由于独特的抗菌特性，纳米银(AgNP)在诸多领域得到广泛应用，但是其生物有效性、动物组织分布及排出尚不清楚。将聚乙烯吡咯烷酮包被的AgNP溶液按照10 mg·kg^-1给雌性SD大鼠灌胃，采用ICP-MS检测SD大鼠组织、粪便及尿液中总银浓度。结果表明，AgNP通过小肠吸收后，可以通过血液循环快速分布在肝、肾、脾、肺、脑等靶器官。灌胃后1 h，大鼠各组织中总银浓度达到最大值(肝、肾、脾、肺、脑中银浓度分别为(0.29 ±0.13) mg·kg^-1、(0.23 ±0.04) mg·kg^-1、(0.17 ±0.05) mg·kg^-1、(0.11 ±0.01) mg·kg^-1、(0.06 ±0.02) mg·kg^-1)，之后银浓度随时间而降低，直至和对照组无显著性差异。在灌胃途径下，AgNP对SD大鼠的有效性为8.5%，且73%的AgNP是通过粪便的途径排出体外。
关键词: AgNP/
SD大鼠/
累积/
生物有效性

Abstract:Due to the unique antimicrobial properties, nanosilver (AgNP) are widely applied in many fields. However, its bioavailability, tissue distribution and excretion in rats are less known. Female Sprague-Dawley rats were treated by a single intragastic administration of 10 mg·kg^-1 polyvinylpyrrolidone-AgNPs, and rat tissues, feces and urine were collected periodically to analyze total Ag concentrations by ICP-MS. The results showed that after uptake by gastrointestine, AgNP was transported via the blood circulation and distributed to tissues such as liver, kidney, spleen, lung, and brain. At 1 h after AgNP exposure, total silver accumulation in those tissues peaked (i.e. 0.29 ±0.13 mg·kg^-1, 0.23 ±0.04 mg·kg^-1, 0.17 ±0.05 mg·kg^-1, 0.11 ±0.01 mg·kg^-1 and 0.06 ±0.02 mg·kg^-1 for liver, kidney, spleen, lung and brain, respectively), and declined to background levels over time. Our calculation showed that the bioavailability of AgNP to Sprague-Dawley rats via intragastric administration was 8.5%. Especially, 73% of the dosed AgNP was excreted via feces.
Key words:AgNP/
Sprague-Dawley rats/
accumulation/
bioavailability.