孙成华^1,2,
石爱军^1,2,,,
刘保献^1,2,
张大伟^1,2,
陈添⁴,
刘建武³,
刘康^1,2,
周健楠^1,2,
洪姗姗^1,2
1. 北京市环境保护监测中心, 北京 100048;
2. 大气颗粒物监测技术北京市重点实验室, 北京 100048;
3. 北京师范大学 核科学与技术学院, 北京 100875;
4. 北京市环境保护局, 北京 100048

作者简介: 孙成华(1969-),女,学士,高级工程师,研究方向为环境生物监测,E-mail:sunchenghua@bjmemc.com.cn.

通讯作者: 石爱军,cems@bjmemc.com.cn ;

基金项目: 北京市环境保护监测中心自筹经费课题（2014-017）


中图分类号: X171.5

Application of Luminescent Bacteria in the Acute Toxicity Test of the Water-Soluble Constituents of PM_2.5

Sun Chenghua^1,2,
Shi Aijun^1,2,,,
Liu Baoxian^1,2,
Zhang Dawei^1,2,
Chen Tian⁴,
Liu Jianwu³,
Liu Kang^1,2,
Zhou Jiannan^1,2,
Hong Shanshan^1,2
1. Beijing Municipal Environmental Monitoring Center, Beijing 100048, China;
2. Beijing Key Laboratory of Airborne Particulate Matter Monitoring Technology, Beijing 100048, China;
3. The College of Nuclear Science and Technology of Beijing Normal University, Beijing 100875, China;
4. Beijing Environmental Protection Agency, Beijing 100048, China

Corresponding author: Shi Aijun,cems@bjmemc.com.cn ;

CLC number: X171.5

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摘要:为科学评估PM_2.5对生物体综合生物效应,研究建立了利用费氏弧菌检测PM_2.5水溶性提取液的毒性测试方法,确立了PM_2.5样品提取液发光细菌毒性测试实验质量控制办法。对春节烟花爆竹燃放和沙尘污染过程的PM_2.5实样测试表明:烟花爆竹燃放期间的PM_2.5样品提取液发光抑制率值与微量金属元素等有毒有害组分浓度显著相关;沙尘污染期间的PM_2.5样本提取液中地壳元素浓度和发光抑制率值显著不相关。
关键词: 发光细菌/
生物毒性测试/
发光抑制率/
PM_2.5

Abstract:The fine particulate matter (PM_2.5) can be breathed into lungs and threatens human health. For scientific assessment of the comprehensive biological effects of PM_2.5, a simple method with the laboratory quality control standards for testing the toxicity of the PM_2.5 water-soluble components was established by using Vibrio fischeri as an indicator bacterium. A series of toxicity tests performed on this luminescent bacterium were carried out with the PM_2.5 samples from the Spring Festival fireworks and the dust pollution, respectively. Our results showed that the luminous inhibition rate had a significant correlation with the concentrations of poisonous and harmful trace metal elements discharged by the fireworks and crackers during the Spring Festival, whereas the luminous inhibition rate was not associated with the concentrations of the earth's crust elements in the PM_2.5 extracting solution.
Key words:luminescent bacteria/
bio-toxicity test/
luminous inhibition rate/
fine particulate matter.