汤花梅^1,2,
魏婷^1,2,
张超^1,2,
刘玉倩^1,2,
汪艳¹,
张晓华¹,
袁丽¹,
戴和平^1,,
1. 中国科学院水生生物研究所 淡水生态和生物技术国家重点实验室, 武汉 430072;
2. 中国科学院大学, 北京 100049

作者简介: 汤花梅(1989-),女,硕士,研究方向为酵母生物传感器监测基因毒性研究,E-mail:sjtangyuan@sina.cn.

通讯作者: 戴和平,hpdai@ihb.ac.cn

基金项目: 国家自然科学基金重点项目（21037004）；淡水生态和生物技术国家重点实验项目（2012FBZ10）


中图分类号: X171.5

Utilization of Super-sensitive Yeast HUG1-yEGFP Biosensor to Screen Genotoxic Compounds

Tang Huamei^1,2,
Wei Ting^1,2,
Zhang Chao^1,2,
Liu Yuqian^1,2,
Wang Yan¹,
Zhang Xiaohua¹,
Yuan Li¹,
Dai Heping^1,,
1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: Dai Heping,hpdai@ihb.ac.cn

CLC number: X171.5

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摘要:为了扩展本实验室构建的响应于DNA损伤信号的超敏感酵母HUG1-yEGFP生物传感器的检测范围并积累参考数据,为实际应用打下实验基础,本研究对26种与环境及人类生活息息相关的化合物的基因毒性进行了检测,筛选出8种具有基因毒性的化合物,并将其结果与现有微生物检测系统的检测结果进行了对比。结果显示,超敏感酵母HUG1-yEGFP生物传感器的检测结果与以细菌为宿主细胞的Ames试验或SOS显色法的检测结果的吻合度高于50%,与以酵母为宿主细胞的GSA检测系统的检测结果的吻合度高于85%。以真核生物酵母为宿主的检测系统与以原核生物细胞为宿主的检测系统的结果的差异反映了它们抗胁迫机制的不同。本研究应用超敏感酵母HUG1-yEGFP生物传感器特异性检测出3种化合物为基因毒性阳性,这3种化合物是代森锰锌、孔雀石绿及丙烯酰胺,均被报道具有潜在致癌性。本研究结果表明,超敏感酵母HUG1-yEGFP生物传感器可以有效地对环境污染有关的基因毒性化合物进行检测,可以作为其他现有检测系统的补充,应用于环境化学污染的健康风险评价。
关键词: 环境污染/
酵母/
生物传感器/
基因毒性/
健康风险评价

Abstract:In order to extend the detection range of super-sensitive DNA damage responded yeast HUG1-yEGFP biosensor which was constructed by our laboratory, and to accumulate more relevant data for practical application, 26 kinds of chemicals which were widely distributed in the environment and human lives were detected and 8 kinds of genotoxic compounds were screened out. Compared with three existed microbiological whole-cell sensing systems, the detection results in this study matched over 50% of the data from Ames test and SOS colorimetric test based on bacterial cell, and over 85% of the data from the GSA test based on yeast cell. The differences of the results between the test systems based on eukaryotic yeast cell and prokaryotic bacteria cells reflected the different mechanisms of stress resistance. In this study, three kinds of genotoxicity-positive compounds, mancozeb, malachite green and acrylamide which were reported as potential carcinogens, were specifically detected by super-sensitive yeast HUG1-yEGFP biosensor. The results of the study indicated that super-sensitive yeast HUG1-yEGFP biosensor can be used to effectively detect genotoxic compounds which were related to environmental pollution. Therefore, they are valuable in environmental health risk assessment for chemical pollutant as a complementary method for other existed detection systems.
Key words:environmental pollution/
yeast/
biosensor/
genotoxicity/
health risk assessment.