王吉龙^1,2,
韩莹³,
王萌萌^1,2,
孙湖泊⁴,
黄沛力^1,2,,,
孙志伟^1,2
1. 首都医科大学公共卫生学院, 北京 100069;
2. 环境毒理学北京市重点实验室, 北京 100069;
3. 中国食品药品检定研究院, 北京 100050;
4. 北京体育大学, 北京 100084

作者简介: 王吉龙(1988-),男,硕士研究生,研究方向为卫生毒理学,Email:wangjilong21@126.com.

通讯作者: 黄沛力,huangpl@ccmu.edu.cn ;

基金项目: 国家自然科学基金项目（81273131）；北京市教育委员会科技发展计划重点项目（KZ201510025027）；首都医科大学优秀学术带头人及团队交流培养项目


中图分类号: X171.5

Influences of CdTe Quantum Dots on Ultrastructure and Enzymatic Activities of Mice Liver

Wang Jilong^1,2,
Han Ying³,
Wang Mengmeng^1,2,
Sun Hubo⁴,
Huang Peili^1,2,,,
Sun Zhiwei^1,2
1. School of Public Health, Capital Medical University, Beijing 100069, China;
2. Beijing Key Laboratory of Environmental Toxicology, Beijing 100069, China;
3. National Institutes for Food and Drug Control, Beijing 100050, China;
4. Beijing Sport University, Beijing 100084, China

Corresponding author: Huang Peili,huangpl@ccmu.edu.cn ;

CLC number: X171.5

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摘要:探讨了碲化镉量子点(cadmium telluride quantum dots,CdTe QDs)对小鼠肝组织超微结构及琥珀酸脱氢酶(succinate dehydrogenase,SDH)和三磷酸腺苷酶(adenosine triphosphatase,ATPase)活性的影响。将20只雄性ICR小鼠随机分成QDs染毒组和对照组,采用尾静脉注射方式进行一次性染毒,染毒组每只小鼠注射2 μmol·kg^-1体重的CdTe QDs (以Cd²⁺的摩尔浓度计算)。每5只为一组分别在染毒后的1 d、3 d和7 d处死小鼠,另外5只注射生理盐水作为对照组。取部分肝组织在透射电镜下观察其超微结构变化并使用试剂盒测定肝脏中SDH、Na⁺/K⁺-ATP酶、Ca²⁺/Mg²⁺-ATP酶的活性。结果显示CdTe QDs暴露后染毒组小鼠肝细胞胞浆疏松,线粒体出现肿胀、空泡、嵴减少等结构变化,表明肝细胞受到损伤且染毒1 d后损伤最严重;肝脏中染毒组SDH活性与对照组相比显著降低(P<0.05);染毒1 d后,Na⁺/K⁺-ATP酶和Ca²⁺/Mg²⁺-ATP酶活性与对照组相比显著升高,随着染毒后时间的延长,其活性呈现下降的变化趋势。单次量注射CdTe QDs能够引起小鼠肝脏损伤,抑制SDH活性,引起ATP酶活性改变,这些症状在暴露时间内呈现恢复趋势。
关键词: 碲化镉量子点/
小鼠/
肝组织/
超微结构/
琥珀酸脱氢酶/
ATP酶

Abstract:In the present study, influences of cadmium telluride quantum dots (CdTe QDs) on the ultrastructure and the activities of succinate dehydrogenase and adenosine triphosphatase in liver of mice were examined. Twenty ICR mice were randomly divided into exposed groups and control group, and injected with 2 μmol·kg^-1 weight of CdTe QDs (as Cd) by tail intravenous injection (IOCV). Mice injected with normal saline were used as the control. Each of 5 mice was sacrificed at 1 d, 3 d and 7 d, respectively. Liver tissue was sampled to observe the ultrastructural changes by transmission electron microscopy (TEM) and homogenized to measure enzymatic activities of SDH, Na⁺/K⁺-ATPase, Ca²⁺/Mg²⁺-ATPase using assay kits. The TEM results showed that mitochondria swelling, vacuole, crest reduction occurred after CdTe QDs exposure, indicating the hepatocytes damage, especially for 1 d exposure. The activities of SDH in the treated groups were significantly lower than that in the control group (P<0.05). The activities of Na⁺/K⁺-ATPase and Ca²⁺/Mg²⁺-ATPase in the liver tissue were significantly higher than those in the control group at 1 d exposure. As the exposure time increasing, the activities of ATPase showed a downward trend. We concluded that single dose of CdTe QDs exposure could induce mice liver damage, affect activities of both SDH and ATP enzyme, which could gradually recovered.
Key words:cadmium telluride quantum dots/
mice/
liver tissue/
ultrastructure/
succinate dehydrogenase/
ATPase.