袁婷婷¹,
王新亮²,
董慧峪^3,,
1. 北京医院检验科, 北京 100730;
2. 天津市城市规划设计研究院, 天津 300201;
3. 中国科学院生态环境研究中心, 北京 100085

作者简介: 袁婷婷(1985-),女,检验医师,研究方向为细胞生物毒理学,E-mail:ttyuan.ok@163.com.

通讯作者: 董慧峪,hydong@rcees.ac.cn

基金项目: 国家自然科学基金课题（51408590）


中图分类号: X171.5

Mutagenic Analysis of Five Haloacetamides Disinfection By-products in the Tk Gene of Mouse Lymphoma Cells

Yuan Tingting¹,
Wang Xinliang²,
Dong Huiyu^3,,
1. Laboratory Department, Beijing Hospital, Beijing 100730, China;
2. Tianjin Urban Planning & Design Institute, Tianjin 300201, China;
3. Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China

Corresponding author: Dong Huiyu,hydong@rcees.ac.cn

CLC number: X171.5

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摘要:卤代乙酰胺类(HAcAm)消毒副产物(DBPs)是饮用水中新兴含氮类DBPs之一。为探究HAcAms的致突变性,选择小鼠淋巴瘤细胞作为受试细胞,分别考察了一氯代乙酰胺、二氯代乙酰胺、三氯代乙酰胺、一碘代乙酰胺、二碘代乙酰胺(DIAcAms)共5种HAcAms对小鼠淋巴瘤细胞Tk基因突变的影响。结果表明,在0.05~20 μmol·L^-1暴露浓度下,5种HacAms类DBPs对小鼠淋巴瘤细胞呈现出一定的细胞毒性,随着暴露浓度的升高,细胞毒性增强。暴露4 h后,5种HacAms中只有DIAcAms呈现出显著的致突变性(暴露剂量高于5 μmol·L^-1),且以小集落为主。上述研究结果为研究HacAms对哺乳动物细胞的致突变作用提供了基础数据。
关键词: 卤代乙酰胺/
小鼠淋巴瘤细胞/
突变分析

Abstract:Haloacetamide (HAcAm) is one of the emerging nitrogeneous disinfection by-products (DBPs) in drinking water. To evaluate the mutagenic potential of HAcAm, effects of 5 HAcAms including chloroacetamide, dichloroacetamide, trichloroacetamide, iodoacetamide and diiodoacetamide (DIAcAm) on thymidine kinase (Tk) gene in the well-validated mouse lymphoma assay were investigated in this study. The results showed that with the exposure levels from 0.05 to 20 μmol·L^-1, all the 5 HAcAms investigated exhibited cytotoxicity, which increased with the elevated exposure level. After 4 h of exposure, only DIAcAm increased the frequency of mutant colonies, with a higher proportion of small colonies. These results of HAcAms provided basic data for the evaluation of mutagenic potential to mammalian cells.
Key words:haloacetamide/
mouse lymphoma cells/
mutation assay.