郭肖颖¹,
王磊¹,
李敏²,
王斌¹,
赵竑绯¹,
李虹颖²,
徐文静¹,
施六林^1,,
1. 安徽省农业科学院 农业工程研究所, 合肥 230031;
2. 安徽省农业科学院 土壤与肥料研究所, 合肥 230031

作者简介: 郭肖颖(1979-),女,副研究员,研究方向为农业生态和环境毒理学,E-mail:gxy2@mail.ustc.edu.cn.

通讯作者: 施六林,shiliulin386@126.com

基金项目: 安徽省科技攻关计划项目（1301042115）；安徽省农业科学院科技创新团队建设项目（14C1308）；国家自然科学基金（21407002）；安徽省自然科学基金（1508085MB39）；安徽省农业科学院学科建设项目（14A1009）


中图分类号: X171.5

Environmental Toxicology Study of Trace Water Samples with Caenorhabditis elegans

Guo Xiaoying¹,
Wang Lei¹,
Li Min²,
Wang Bin¹,
Zhao Hongfei¹,
Li Hongying²,
Xu Wenjing¹,
Shi Liulin^1,,
1. Institute of Agricultural Engineering, Anhui Academy of Agricultural Science, Hefei 230031, China;
2. Institute of Soil and Fertilizer, Anhui Academy of Agricultural Science, Hefei 230031, China

Corresponding author: Shi Liulin,shiliulin386@126.com

CLC number: X171.5

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摘要:目前,环境水样个体水平的毒理学检测成本较高、耗时长,且检测后的水样若处理不当易造成环境的二次污染。通过使用易于培养和观察、个体微小的模式生物-秀丽隐杆线虫作为实验对象,利用毛细玻璃管的虹吸原理将线虫暴露于微量的环境水样进行染毒培养,实现微量水样在个体水平的毒理学评价。为了验证该方法的可行性,检测了暴露组线虫与对照组线虫的生殖腺细胞损伤水平、活性氧自由基(ROS)产生量、氧化应激水平、脱氧核糖核酸(DNA)损伤水平等生物终点的差异性。研究结果有助于为微量环境水样在个体水平的毒理学分析提供一种快速、可行、廉价的评价手段。
关键词: 秀丽隐杆线虫/
微量环境水样/
毒理学评价/
评价体系

Abstract:In the present, test methods used for environmental water sample toxicology evaluation on individual scale are usually costly and time consuming, and without appropriate treatment, processed water samples would cause secondary pollution. Toxicology evaluation of trace water samples was realized with a kind of tiny model organisms, Caenorhabditis elegans, as experiment subjects who are easy to cultivate and observe; C. elegans could be exposed to trace environment water samples through siphon principle of glass capillary for toxicity cultivation. In order to verify the applicability of this evaluation method, biological end points such as germline cell apoptosis, ROS generation, SOD-3 expression and DNA damage of C. elegans were measured. This research helps to provide a swift, applicable and economical evaluation method for individual scale toxicology evaluation in trace environmental water sample.
Key words:Caenorhabditis elegans/
trace environmental water sample/
toxicology evaluation/
assessment system.