Li Ran¹,
Qiu Xinghua^1,,,
Yang Qiaoyun¹,
Li Keqiu²,
Li Guang^2,,
1. State Key Joint Laboratory for Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering and Center for Environment and Health, Peking University, Beijing 100871, P. R. China;
2. Basic Medical College, Tianjin Medical University, Tianjin 300070, P. R. China

作者简介: Li Ran,PhD,E-mail:ranli28@126.com.

通讯作者: Qiu Xinghua,xhqiu@pku.edu.cn ; Li Guang,lig@tijmu.edu.cn

基金项目: National Natural Science Foundation of China (21322705,41121004,21190051,and 21177091)

MiR-125a-5p is Upregulated in Plasma of Residents from An Electronic Waste Recycling Site

Li Ran¹,
Qiu Xinghua^1,,,
Yang Qiaoyun¹,
Li Keqiu²,
Li Guang^2,,
1. State Key Joint Laboratory for Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering and Center for Environment and Health, Peking University, Beijing 100871, P. R. China;
2. Basic Medical College, Tianjin Medical University, Tianjin 300070, P. R. China

Corresponding authors: Qiu Xinghua,xhqiu@pku.edu.cn ; Li Guang,lig@tijmu.edu.cn

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摘要:The mechanism of health effects caused by organohalogen pollutants,e.g.,toxins from electronic waste (e-waste),is poorly understood.We supposed that microRNAs (miRNAs),an important post-transcriptional regulator,could play a role in this process.In this study,fasting peripheral blood samples were collected from residents living at an e-waste site in northern China and a nearby reference population.Concentrations of e-waste related organohalogen pollutants in plasma from the exposure group were higher than the corresponding measurement in the reference group.Correspondingly,sixty miRNAs in plasma showed > 2-fold change between the two groups in microarray analysis.Among them,miR-125a-5p was confirmed to be upregulated by qRT-PCR and its validated targets were enriched in responses to xenobiotics and cancer related pathways.Furthermore,significant positive correlations were found between levels of miR-125a-5p in plasma and reactive oxygen species (ROS) in polymorphonuclear neutrophil leuK_ocytes (P<0.05).These evidences suggested oxidative stress might be an intermediate between e-waste related POPs exposure and alteration of plasma miRNA.
关键词: e-waste originated pollutants/
microRNA/
miR-125a-5p/
epigenetics/
oxidative stress

Abstract:The mechanism of health effects caused by organohalogen pollutants, e.g., toxins from electronic waste (e-waste), is poorly understood. We supposed that microRNAs (miRNAs), an important post-transcriptional regulator, could play a role in this process. In this study, fasting peripheral blood samples were collected from residents living at an e-waste site in northern China and a nearby reference population. Concentrations of e-waste related organohalogen pollutants in plasma from the exposure group were higher than the corresponding measurement in the reference group. Correspondingly, sixty miRNAs in plasma showed > 2-fold change between the two groups in microarray analysis. Among them, miR-125a-5p was confirmed to be upregulated by qRT-PCR and its validated targets were enriched in responses to xenobiotics and cancer related pathways. Furthermore, significant positive correlations were found between levels of miR-125a-5p in plasma and reactive oxygen species (ROS) in polymorphonuclear neutrophil leuK_ocytes (P<0.05). These evidences suggested oxidative stress might be an intermediate between e-waste related POPs exposure and alteration of plasma miRNA.
Key words:e-waste originated pollutants/
microRNA/
miR-125a-5p/
epigenetics/
oxidative stress.