韩笑笑^1,2,
赵建^1,2,
王震宇^1,2,
隋海君^1,2,
徐立娜^1,2,,
1. 中国海洋大学环境科学与工程学院, 青岛 266100;
2. 青岛海洋科学与技术国家实验室 海洋生态与环境科学功能实验室, 青岛 266071

作者简介: 韩笑笑(1991-),女,研究方向为污染物的环境地球化学,E-mail:x_smile901@163.com.

通讯作者: 徐立娜,xln1984@126.com

基金项目: 国家自然科学基金(41073067
41120134004)


中图分类号: X171.5

Effect of TiO₂ Engineered Nanoparticles at Different Aging Times on the Growth of Zea mays L. in Soil

Han Xiaoxiao^1,2,
Zhao Jian^1,2,
Wang Zhenyu^1,2,
Sui Haijun^1,2,
Xu Lina^1,2,,
1. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;
2. Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China

Corresponding author: Xu Lina,xln1984@126.com

CLC number: X171.5

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摘要:为探讨老化时间对TiO₂纳米颗粒(nanoparticles,NPs)生物有效性的影响，研究了不同老化时间的TiO₂ NPs (0~120 d)对玉米幼苗生长的影响、在玉米体内的吸收及其在植株不同部位的存在位点等。研究发现，不同浓度的TiO₂ NPs (1 000 mg·kg^-1和2 000 mg·kg^-1)加入到土壤中，对玉米幼苗干鲜重没有明显的影响，但老化时间小于60 d时，对玉米幼苗株高有一定的抑制效应，老化60 d之后，随着老化时间的继续延长，毒性逐渐降低，最后趋于稳定。老化60 d时，TiO₂ NPs处理的玉米幼苗根冠增大，玉米幼苗体内产生H₂O₂的累积。在TiO₂老化土壤中生长的玉米幼苗根系和地上部均有Ti的累积，1 000 mg·kg^-1的TiO₂ NPs在玉米幼苗根部的生物累积系数达到35.4%，在地上部为13.6%，在玉米植株体内的转运系数为0.38；通过TEM观察，TiO₂ NPs可以进入到玉米幼苗体内，并存在于根细胞的细胞质和叶绿体膜上，在叶片细胞的液泡和细胞核中也发现有TiO₂ NPs的存在。上述研究结果为客观评价TiO₂ NPs的生态风险提供了有用信息。
关键词: TiO₂ NPs/
玉米幼苗/
生长效应/
吸收转运

Abstract:To investigate the effect of aging time on the biological effectiveness of TiO₂ nanoparticles (NPs) in soil, the growth of maize seedlings, and the uptake and location of TiO₂ NPs in maize seedlings in the TiO₂ NPs treatment (1 000 mg·kg^-1 and 2 000 mg·kg^-1) with different aging times were studied. The results suggested that TiO₂ NPs at all the tested aging times (0~120 d) had no negative effects on the dry or fresh weight of maize seedlings in soil. For the height of maize seedling, significant decrease was observed when the aging time of TiO₂ NPs was less than 60 days. The inhibition on the height of maize seedling was decreased after aging over 60 d. After exposure to 60 d aged TiO₂ NPs in soil, the root cap of maize seedling was increased and H₂O₂ was accumulated. Ti was detected in the roots and shoots of maize after TiO₂ NPs exposure, indicating that TiO₂ NPs could be taken up in maize seedlings, and then be transported from root to shoot. After exposure to 1 000 mg·kg^-1 TiO₂ for 60 d, the bioaccumulation coefficient in maize seedling root was 35.4% and 13.6% in shoot. The transfer coefficient in maize seedlings was 0.38. As observed by TEM, TiO₂ NPs could exist in chloroplast membrane and nucleus of root cells, and vacuoles and nucleus of leaf cells.
Key words:TiO₂ nanoparticles/
maize seedlings/
growth effect/
transfer.