马萍^1,2,
李金泉²,
晏彪²,
刘旭东²,
廖文莉¹,
杨旭²,
武阳^1,2,,
1. 湖北科技学院基础医学院环境生物医学实验室, 咸宁 437100;
2. 华中师范大学生命科学学院环境生物医学实验室, 武汉 430079

作者简介: 马萍(1968-),女,教授,研究方向为环境毒理学,Email:mping68@126.com.

通讯作者: 武阳,wysj2007@126.com

基金项目: 湖北省自然科学基金面上项目(2014CFB284)
国家自然青年科学基金项目(21507026)
湖北科技学院博士启动基金项目(BK1412)


中图分类号: X171.5

Study on Oxidative Damage of Mouse Lung Cells Induced by Plasticizers Diisononyl Phthalate

Ma Ping^1,2,
Li Jinquan²,
Yan Biao²,
Liu Xudong²,
Liao Wenli¹,
Yang Xu²,
Wu Yang^1,2,,
1. Lab of Environmental Biomedicine, School of Basic Medicine, Hubei University of Science and Technology, Xianning 437100, China;
2. Lab of Environmental Biomedicine, College of Life Science, Huazhong Normal University, Wuhan 430079, China

Corresponding author: Wu Yang,wysj2007@126.com

CLC number: X171.5

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摘要:为研究增塑剂邻苯二甲酸二异壬酯(diisononyl phthalate,DINP)对小鼠肺组织的氧化损伤作用，以昆明小鼠为受试动物，随机分为5组，包括1个阴性对照组(生理盐水)和4个DINP染毒组(0.2、2、20和200 mg·kg^-1)，灌胃14 d。光镜下发现小鼠肺组织形态随染毒剂量的增加，小鼠肺细胞的病理损伤越严重。随着DINP染毒剂量的增加，肺组织匀浆活性氧(reactive oxygen species,ROS)、丙二醛(malondialdehyde,MDA)含量和肺组织细胞DNA-蛋白质交联(DNA-protein crosslink,DPC)系数逐渐上升，还原型谷胱甘肽(glutathione,GSH)含量逐渐降低，各指标呈一定的剂量-效应关系。染毒剂量为20 mg·kg^-1时，ROS和MDA含量差异有统计学意义(P<0.05，P<0.01)；染毒剂量为200 mg·kg^-1时，上述指标差异均有统计学意义(P<0.01)。结果表明，较高剂量(≥20 mg·kg^-1)的DINP能造成小鼠肺组织的氧化损伤和病理损伤。
关键词: 邻苯二甲酸二异壬酯/
小鼠/
活性氧/
还原型谷胱甘肽/
丙二醛/
DNA-蛋白质交联/
氧化损伤

Abstract:To evaluate oxidative damages of plasticizers diisononyl phthalate (DINP) on mouse lung tissue cell, Kunming mice were divided randomly into five groups and administered daily for two weeks. The groups included one saline group and four DINP groups (0.2, 2, 20 and 200 mg·kg^-1). Lung tissue sections were isolated and stained for pathological observations under the microscope. Meanwhile, contents of reactive oxygen species (ROS), glutathione (GSH) and malondialdehyde (MDA) in lung tissue homogenates, and DNA-protein crosslink (DPC) coefficients in the lung cell suspensions were measured. Results showed that lung tissue injury aggravated with the increase of DINP exposure concentration, with the contents of ROS, MDA and DPC coefficients increased in a dose-dependent manner, whereas GSH content decreased accordingly. In the 20 mg·kg^-1 exposure group, ROS and MDA contents were higher compared with the control group (P<0.05, P<0.01). In the 200 mg·kg^-1 exposure group, there were significant differences in levels of each biomarker compared with the control group (P<0.01). Data suggest that DINP at certain doses (≥ 20 mg·kg^-1) can induce oxidative damage and pathological damages in mice lung tissue cell.
Key words:diisononyl phthalate/
mice/
reactive oxygen species/
glutathione/
malondialdehyde/
DNA-protein crosslinks/
oxidative damage.