Zhang Hecai,
Liu Tongyi,
Liu Yanfang,
Zhang Jie,
Wang Zhige,
Chen Guangwen^,
College of Life Sciences, Henan Normal University, Xinxiang 453007, China

作者简介: Zhang Hecai (1977-), PhD, Associate Professor in Henan Normal University. Main research interests are planarian molecular systematics and toxicology. E-mail:zhcai9339@163.com.

通讯作者: Chen Guangwen,chengw0183@sina.com

基金项目: This work was supported by the National Natural Science Foundation of China (31471965), the Natural Science Foundation of Henan Province (142300410457) and the National Student Innovation Training Project of Henan Normal University (201410476051)


中图分类号: X171.5

Definition of Planarian Mortality in an Acute Toxicity Test: A Case Study on Dugesia japonica Exposed to 1-Octyl-3-methylimidazolium Bromide

Zhang Hecai,
Liu Tongyi,
Liu Yanfang,
Zhang Jie,
Wang Zhige,
Chen Guangwen^,
College of Life Sciences, Henan Normal University, Xinxiang 453007, China

Corresponding author: Chen Guangwen,chengw0183@sina.com

CLC number: X171.5

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摘要:In order to define the mortality criteria of planarian objectively, a case study of Dugesia japonica exposed to 1-octyl-3-methylimidazolium bromide ([C₈mim]Br) was performed followed by a recovery culture. The results showed that defining planarian mortality in light of its body disintegration was appropriate. If the disintegrated tissue of a planarian was more than 1/4 of its body length, it would completely degenerate. However, a planarian would regenerate the lost tissue and return to normal after a few days' recovery culture if the disintegrated part was less than 1/4 of body length. Therefore, we propose to use body disintegration as the endpoint of planarian mortality, i.e., 1/4 body length degenerated is the critical threshold of mortality and survival of planarians when exposed to toxicants. This criterion could be adapted in the standardization of testing protocols and comparability of acute toxicity test or other toxicological research using planarian as the test animal.
关键词: 1-octyl-3-methylimidazolium bromide/
planarian/
acute toxicity/
mortality criterion/
definition

Abstract:In order to define the mortality criteria of planarian objectively, a case study of Dugesia japonica exposed to 1-octyl-3-methylimidazolium bromide ([C₈mim]Br) was performed followed by a recovery culture. The results showed that defining planarian mortality in light of its body disintegration was appropriate. If the disintegrated tissue of a planarian was more than 1/4 of its body length, it would completely degenerate. However, a planarian would regenerate the lost tissue and return to normal after a few days’ recovery culture if the disintegrated part was less than 1/4 of body length. Therefore, we propose to use body disintegration as the endpoint of planarian mortality, i.e., 1/4 body length degenerated is the critical threshold of mortality and survival of planarians when exposed to toxicants. This criterion could be adapted in the standardization of testing protocols and comparability of acute toxicity test or other toxicological research using planarian as the test animal.
Key words:1-octyl-3-methylimidazolium bromide/
planarian/
acute toxicity/
mortality criterion/
definition.