AssociateInvestigator,NIBS,Beijing,ChinaPhone：010-80726688-8550
Fax：010-80728592
E-mail：yekeqiong@nibs.ac.cn
教育经历Education1995浙江大学生物学学士
B.S.,DepartmentofBiologicalScienceandTechnology,ZhejiangUniversity,Hangzhou,China.
2000中科院生物物理所博士
Ph.D.,MolecularBiology,InstituteofBiophysics,ChineseAcademyofSciences,Beijing,China(Dr.JinfengWang)
工作经历ProfessionalExperience2011-北京生命科学研究所高级研究员AssociateInvestigator,NationalInstituteofBiologicalSciences,Beijing.
2005-2011北京生命科学研究所研究员
AssistantInvestigator,NationalInstituteofBiologicalSciences,Beijing.
2001-2005斯隆癌症研究所博士后
PostdocwithDr.DinshawJ.Patel.MemorialSloan-KetteringCancerCenter,NewYork,USA.
研究概述：我们实验室主要研究蛋白质和核糖核酸之间的相互作用。核糖核酸除了在基因表达中作为信使,还行使许多其他的功能。在各种生物体中已经发现了很多非编码的核糖核酸，它们不翻译成蛋白质，却在分子结构，反应催化，基因调节中发挥重要作用。这些非编码核糖核酸通常和蛋白质形成复合物行使功能。实验室主要的研究领域包括核酸干扰，这是由长度约为21个核甘酸的微小核糖核酸介导的基因调控过程。我们实验室将研究这些微小核酸生物发生机理，工作原理和调控途径。我们还研究由核糖核酸引导的核糖核酸修饰系统，如催化核糖甲基化的C/DRNP和假尿嘧啶化的H/ACARNP。实验室主要使用X光晶体学，核磁共振等结构方法以及生化分析作为研究手段。ResearchDescription:OurlaboratorymainlystudiesinteractionsbetweenproteinandRNA.RNAsplaydiverserolesincellsinadditiontotheirrolesingeneexpression.Alargenumberofnon-codingRNAsthatarenevertranslatedintoproteinhavebeenidentifiedinvariousorganisms.Non-codingRNAsfunctionasstructural,catalyticandregulatorymoleculesandfrequentlyworkincollaborationwithproteinsbyformingRNAproteincomplexes(RNPs).OneofourmajorinterestisRNAsilencing(popularlyknownasRNAi),wheresmallRNAsofabout21nucleotidesinlengthguidegeneregulation.WorkonthistopicisconcernedwithunderstandingsmallRNAgeneration,functionandregulation.OurlabisalsointerestedinthestructureandfunctionofRNA-guidedRNAmodificationenzymes,namelyboxC/DandH/ACARNPsthatdirectribose2'-o-methylationandpseudouridylation,respectively.Structuralapproachessuchasx-raycrystallographyandNMR,combinedwithbiochemicaltechniques,areusedtostudytheseproblems.Publications：1.LimanZhang,JinzhongLinandKeqiongYe(2013)StructuralandfunctionalanalysisoftheU3snoRNAbindingproteinRrp9.RNAdoi:10.1261/rna.037580.112,Mar.18,20132.SanduoZheng#,JiaWang#,YingangFeng#,*,JinfengWangandKeqiongYe*(2012)SolutionstructureofMSL2CXCdomainrevealsanunusualZn3Cys9clusterandsimilaritytopre-SETdomainsofhistonelysinemethyltransferases.PLoSONE,7(9):e45437.doi:10.1371/journal.pone.00454373.XinxingYang,JinqiDuan,ShuangLi,PengWang,ShoucaiMa,KeqiongYe*andXinShengZhao*(2012)KineticandthermodynamiccharacterizationofthereactionpathwayofboxH/ACARNA-guidedpseudouridineformation.NucleicAcidsRes.40:10925-36.doi:10.1093/nar/gks8824.BingYang#,Yan-JieWu#,MingZhu#,Sheng-BoFan,JinzhongLin,KunZhang,ShuangLi,HaoChi,Yu-XinLi,Hai-FengChen,Shu-KunLuo,Yue-HeDing,Le-HengWang,ZhiqiHao,Li-YunXiu,SheChen,KeqiongYe,Si-MinHe*andMeng-QiuDong*(2012)Identificationofcross-linkedpeptidesfromcomplexsamples.NatureMethod.9:904-906.doi:10.1038/nmeth.20995.XingZhuandKeqiongYe(2012)CrystalstructureofCmr2suggestsanucleotidecyclase-relatedenzymeintypeIIICRISPR-Cassystems.FEBSLetters.10.1016/j.febslet.2012.02.036.6.WeiZhang,YangZhao,YanGuoandKeqiongYe(2012)Plantactin-bindingproteinSCAB1isdimericactincross-linkerwithatypicalpleckstrinhomologydomain.J.Biol.Chem.doi:10.1074/jbc.M112.341347.7.ShukunLuoandKeqiongYe(2012)Dimerization,butnotphosphothreoninebinding,isconservedbetweentheforkhead-associateddomainsofDrosophilaMU2andhumanMDC1.FEBSLetters.586:344-349.doi:10.1016/j.febslet.2012.01.023.8.JinpingLiu#,ShukunLuo#,HongchangZhao,JiLiao,JingLi,ChunyingYang,BoXu,DavidF.Stern,XingzhiXu*andKeqiongYe*(2012)Structuralmechanismofthephosphorylation-dependentdimerizationoftheMDC1forkhead-associateddomain.NucleicAcidsRes.doi:10.1093/nar/gkr1296(#equalcontribution,*correspondingauthor)9.ShuangLi,JingqiDuan,DandanLi,ShoucaiMaandKeqiongYe2011.StructureoftheShq1-Cbf5-Nop10-Gar1complexandimplicationsforH/ACARNPbiogenesisanddyskeratosiscongenita.EMBOJ.doi:10.1038/emboj.2011.42710.ShuangLi,JingqiDuan,DandanLi,BingYang,MengqiuDongandKeqiongYe2011.ReconstitutionandstructuralanalysisoftheyeastboxH/ACARNA-guidedpseudouridinesynthase.GenesDev25:2409-2421.doi:10.1101/gad.175299.11111.JinzhongLin,LimanZhang,ShaomeiLai,KeqiongYe.2011StructureandmolecularevolutionofCDGSHiron-sulfurdomains.PLoSONE,6(9):e24790.doi:10.1371/journal.pone.002479012.YangZhao,ShuangshuangZhao,TonglinMao,XiaoluQu,WanhongCao,LiZhang,WeiZhang,LiuHe,SidiLi,SulinRen,JinfengZhao,GuoliZhu,ShanjinHuang,KeqiongYe,MingYuan,andYanGuo.2011ThePlant-SpecificActinBindingProteinSCAB1StabilizesActinFilamentsandRegulatesStomatalMovementinArabidopsis.PlantCell.doi:10.1105/tpc.111.08654613.BihongGuo,JinzhongLinandKeqiongYe.2011.Structureoftheautocatalyticcysteineproteasedomainofpotyvirushelper-componentproteinase.J.Biol.Chem.286:21937-21943doi:10.1074/jbc.M111.23070613.LinJ.,LaiS.,JiaR.,XuA.,ZhangL.,LuJ.andYeK.2011Structuralbasisofsite-specificribosemethylationbyboxC/DRNAproteincomplexes.Nature469:559-56314.HuangL.,YinP.,ZhuX.,ZhangY.andYeK.2010CrystalstructureandcentromerebindingoftheplasmidsegregationproteinParBfrompCXC100.NucleicAcidsRes.doi:10.1093/nar/gkq91515.ShenM.,XuY.,JiaR.,ZhouX.andYeK.2010Size-independentandnon-cooperativerecognitionofdsRNAbytheRicestripevirusRNAsilencingsuppressorNS3.JMolBiol.404:665-67916.YeK.,JiaR.,LinJ.,JuM.,PengJ.,XuA.andZhangL.2009StructuralorganizationofboxC/DRNA-guidedRNAmethyltransferase.ProcNatlAcadSciUSA,106:13808-1381317.DuanJ.,LiL.,LuJ.,WangW.andYeK.2009.StructuralmechanismofsubstrateRNArecruitmentinH/ACARNA-guidedpseudouridinesynthase.MolCell.34:427-43918.LinJ,ZhouT,YeK,WangJ.CrystalstructureofhumanmitoNEETrevealsdistinctgroupsofironsulfurproteins.PNAS.2007104(37):14640-5.19.Ye,K.2007.H/ACAguideRNAs,proteinsandcomplexes.CurrentOpinioninStructuralBiology.2007,17:287–29220.Li,L.andYe,K.2006.CrystalstructureofanH/ACAboxribonucleoproteinparticle.Nature443:302-307.21.YeK.andPatelD.J..RNAsilencingsuppressorp21ofBeetYellowsVirusformsanRNA-bindingoctamericringstructure.Structure.2005;13,1375-1384.22.*MaJ.,*YeK.andPatelD.J..Structuralmechanismofoverhang-specificsmallinterferingRNArecognitionofthePAZdomain.Nature.2004;429:318-322.(*Equalcontribution)23.YeK.,MalininaL.andPatelD.J..RecognitionofsmallinterferingRNAbyaviralsuppressorofRNAsilencing.Nature.2003;426:874-878.24.YeK.,SerganovA.,HuW.,GarberM.andPatelD.J..Ribosome-associatedfactorYadoptsafoldresemblingadouble-strandedRNAbindingdomainscaffold.Eur.J.Biochem.2002;269:5182-5191.25.YeK.andWangJ..Self-associationreactionofdenaturedstaphylococcalnucleasefragmentscharacterizedbyheteronuclearNMR.J.Mol.Biol.2001;307:309-322.26.YeK.,JingG.andWangJ..Interactionsbetweensubdomainsinthepartiallyfoldedstateofstaphylococcalnuclease.Biochim.Biophys.Acta.2000;1479:123-134.
