土壤杆菌-毕赤酵母耦合培养直接生产热凝胶低聚糖

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李菲菲¹，金树霞¹，朱莉²，詹晓北¹，赵玥¹，刘丽萍¹，高敏杰^1*

1. 江南大学生物工程学院工业生物技术教育部重点实验室，江苏无锡 214122 2. 无锡格莱克斯生物科技有限公司，江苏无锡 214125

收稿日期:2018-11-09修回日期:2018-12-26出版日期:2019-08-22发布日期:2019-08-15
通讯作者:高敏杰

基金资助:国家自然科学基金（极高细胞密度下乙醇历史积累抑制毕赤酵母表达外源蛋白的分子机制及解抑制策略研究）;江南大学自主科研计划-重点项目（高粘度、高密度发酵过程关键技术与装备）;江苏省政策引导类计划（产学研合作）-前瞻性联合研究项目(毕赤酵母高效吸附稀贵金属离子关键技术研发及产业化）

Direct production of curdlan oligosaccharides by coupled fermentation system of Agrobacterium sp.-Pichia pastoris

Feifei LI¹, Shuxia JIN¹, Li ZHU², Xiaobei ZHAN¹, Yue ZHAO¹, Liping LIU¹, Minjie GAO^1*

1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China 2. Wuxi Glyco Biotechology Co., Ltd., Wuxi, Jiangsu 214125, China

Received:2018-11-09Revised:2018-12-26Online:2019-08-22Published:2019-08-15

摘要/Abstract

摘要： 为提高热凝胶低聚糖生产效率，构建了土壤杆菌?毕赤酵母耦合培养体系，其中土壤杆菌代谢产物热凝胶可被毕赤酵母分泌的内切-?-1,3-葡聚糖酶利用直接生产热凝胶低聚糖。用基于不同启动子(AOX1, GAP, FLD)调控的毕赤酵母重组菌株分泌表达内切-β-1,3-葡聚糖酶BGN13.1a，验证其均能有效水解热凝胶得到热凝胶低聚糖。在此基础上，选取GAP启动子调控的毕赤酵母工程菌与土壤杆菌耦合培养，通过设计两种物种之间的共生关系实现稳定共培养并生产聚合度为17?22的热凝胶低聚糖，产量为4.278 g/L。

引用本文

李菲菲金树霞朱莉詹晓北赵玥刘丽萍高敏杰. 土壤杆菌-毕赤酵母耦合培养直接生产热凝胶低聚糖[J]. 过程工程学报, 2019, 19(4): 801-808.
Feifei LI Shuxia JIN Li ZHU Xiaobei ZHAN Yue ZHAO Liping LIU Minjie GAO. Direct production of curdlan oligosaccharides by coupled fermentation system of Agrobacterium sp.-Pichia pastoris[J]. Chin. J. Process Eng., 2019, 19(4): 801-808.

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