Wenjing Li
Jun Li
a State Key Laboratory of North China Crop Improvement and Regulation, College of Life Sciences, Hebei Agricultural University, Baoding, Hebei 071001, China;
b College of Life Sciences, Capital Normal University, Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing 100048, China
Funds: We apologize to those whose work was not cited because of space constraints. This work was financially supported by the National Natural Science Foundation of China (32000454), Provincial Natural Science Foundation of Hebei for Excellent Young Scholar (C2020204062), Program for Young Talents of Hebei Education Department (BJ2021025), and Starting Grant from Hebei Agricultural University (YJ201958).
Received Date: 2021-03-25
Accepted Date:2021-05-06
Rev Recd Date:2021-04-22
Publish Date:2021-08-20
Abstract
Abstract
The ability to precisely inactivate or modify genes in model organisms helps us understand the mysteries of life. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), a revolutionary technology that could generate targeted mutants, has facilitated notable advances in plant science. Genome editing with CRISPR/Cas9 has gained great popularity and enabled several technical breakthroughs. Herein, we briefly introduce the CRISPR/Cas9, with a focus on the latest breakthroughs in precise genome editing (e.g., base editing and prime editing), and we summarize various platforms that developed to increase the editing efficiency, expand the targeting scope, and improve the specificity of base editing in plants. In addition, we emphasize the recent applications of these technologies to plants. Finally, we predict that CRISPR/Cas9 and CRISPR/Cas9-based genome editing will continue to revolutionize plant science and provide technical support for sustainable agricultural development.Keywords: CRISPR/Cas9,
Base editing,
Prime editing,
Loss-of-function,
Gain-of-function,
DNA free
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