Zhuoxin Chen
Zhan Liu
Feng Wang
Xionglei He
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China
More InformationCorresponding author: E-mail address: chenzhx6@mail2.sysu.edu.cn (Zhuoxin Chen);E-mail address: hexiongl@mail.sysu.edu.cn (Xionglei He)
Publish Date:2020-02-25
Abstract
Abstract
There is a growing interest in developing experimental methods for tracking the developmental cell lineages of a complex organism. The recently developed CRISPR/Cas9-based barcoding method is, although highly promising, difficult to scale up because it relies on exogenous barcoding sequences that are engineered into the genome. In this study, we characterized 78 high-quality endogenous sites in the zebrafish genome that can be used as CRISPR/Cas9-based barcoding sites. The 78 sites are all highly expressed in most of the cell types according to single-cell RNA sequencing (scRNA-seq) data. Hence, the barcoding information of the 78 endogenous sites is recovered by the available scRNA-seq platforms, enabling simultaneous characterization of cell type and cell lineage information.Keywords: Zebrafish,
CRISPR/Cas9,
Cell lineage,
Development,
Single-cell RNA sequencing
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http://www.jgenetgenomics.org/article/exportPdf?id=762f36c9-9c41-4786-8b1b-cd263301634c&language=en
