Ying Mao
Rong-Gang Xu
Ruibao Zhu
Ming Zhang
Jin Sun
Da Shen
Ping Peng
Ting Xie
Jian-Quan Ni
aPKU-THU Joint Center for Life Sciences, College of Life Sciences, School of Medicine, Tsinghua University, Beijing, 100084, China
bGene Regulatory Lab, School of Medicine, Tsinghua University, Beijing, 100084, China
cStowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO, 64110, USA
dTsingdao Advanced Research Institute, Tongji University, Qingdao, 266000, China
More InformationCorresponding author: E-mail address: tgx@stowers.org (Ting Xie);E-mail address: nijq@mail.tsinghua.edu.cn (Jian-Quan Ni)
Received Date: 2018-08-15
Accepted Date:2018-10-23
Rev Recd Date:2018-10-02
Available Online: 2019-01-30 Publish Date:2019-01-20
Abstract
Abstract
In the Drosophila ovary, escort cells (ECs) extrinsically control germline stem cell (GSC) maintenance and progeny differentiation. However, the underlying mechanisms remain poorly understood. In this study, we identified 173?EC genes for their roles in controlling GSC maintenance and progeny differentiation by using an in?vivo systematic RNAi approach. Of the identified genes, 10 and 163 are required in ECs to promote GSC maintenance and progeny differentiation, respectively. The genes required for progeny differentiation fall into different functional categories, including transcription, mRNA splicing, protein degradation, signal transduction and cytoskeleton regulation. In addition, the GSC progeny differentiation defects caused by defective ECs are often associated with BMP signaling elevation, indicating that preventing BMP signaling is a general functional feature of the differentiation niche. Lastly, exon junction complex (EJC) components, which are essential for mRNA splicing, are required in ECs to promote GSC progeny differentiation by maintaining ECs and preventing BMP signaling. Therefore, this study has identified the major regulators of the differentiation niche, which provides important insights into how stem cell progeny differentiation is extrinsically controlled.Keywords: Germline stem cells,
Escort cells
PDF全文下载地址:
http://www.jgenetgenomics.org/article/exportPdf?id=26bc50f4-f0ff-45e9-be94-1acd78fa3c0d&language=en
